Biology:Unique molecular identifier
From HandWiki
Unique molecular identifiers (UMIs), or molecular barcodes (MBC) are short sequences or molecular "tags" added to DNA fragments in some next generation sequencing library preparation protocols to identify the input DNA molecule. These tags are added before PCR amplification, and can be used to reduce errors and quantitative bias introduced by the amplification. Applications include analysis of unique cDNAs to avoid PCR biases in iCLIP,[1] variant calling in ctDNA, gene expression in single-cell RNA-seq (scRNA-seq)[2][3] and haplotyping via linked reads[clarification needed].
See also
References
- ↑ König, Julian; Zarnack, Kathi; Rot, Gregor; Curk, Tomaz; Kayikci, Melis; Zupan, Blaz; Turner, Daniel J.; Luscombe, Nicholas M. et al. (July 2010). "iCLIP reveals the function of hnRNP particles in splicing at individual nucleotide resolution". Nature Structural & Molecular Biology 17 (7): 909–915. doi:10.1038/nsmb.1838. ISSN 1545-9985. PMID 20601959.
- ↑ "Counting absolute numbers of molecules using unique molecular identifiers". Nat. Methods 9 (1): 72–4. 2012. doi:10.1038/nmeth.1778. PMID 22101854.
- ↑ "Quantitative single-cell RNA-seq with unique molecular identifiers". Nat. Methods 11 (2): 163–6. 2014. doi:10.1038/nmeth.2772. PMID 24363023.
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