Chemistry:Black Hole Quencher 1
From HandWiki
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| Names | |
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| IUPAC name
2-[N-(2-hydroxyethyl)-4-[[2-methoxy-5-methyl-4-[(4-methyl-2-nitrophenyl)diazenyl]phenyl]diazenyl]anilino]ethanol
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| Other names
BHQ1; BHQ-1
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| Identifiers | |
3D model (JSmol)
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| ChemSpider | |
PubChem CID
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| Properties | |
| C25H28N6O5 | |
| Molar mass | 492.536 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Tracking categories (test):
Black Hole Quencher 1 (BHQ1) is an example of dark quencher, which is used to quench green and yellow dyes, such as 6-carboxyfluorescein (6-FAM), tetrachlorofluorescein (TET), and hexachlorofluorescein (HEX). The role of quenchers is to absorb energy from a fluorophore and to re-emit the energy in the form of either heat (dark quenchers) or visible light (fluorescent quenchers). The absorption range of BHQ1 is from 480 to 580 nm with maximum absorption at 534 nm.[1][2][3][4]
See also
References
- ↑ Didenko, V. V. (November 2001). "DNA probes using fluorescence resonance energy transfer (FRET): designs and applications". BioTechniques 31 (5): 1106–1116, 1118, 1120–1121. doi:10.2144/01315rv02. ISSN 0736-6205. PMID 11730017.
- ↑ Livak, K. J.; Flood, S. J.; Marmaro, J.; Giusti, W.; Deetz, K. (June 1995). "Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization". PCR Methods and Applications 4 (6): 357–362. doi:10.1101/gr.4.6.357. ISSN 1054-9803. PMID 7580930.
- ↑ Yeung, Anthony T.; Holloway, Brian P.; Adams, Pamela Scott; Shipley, Gregory L. (February 2004). "Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR". BioTechniques 36 (2): 266–270, 272, 274–275. doi:10.2144/04362RR01. ISSN 0736-6205. PMID 14989091.
- ↑ Tyagi, Sanjay; Kramer, Fred Russell (March 1996). "Molecular Beacons: Probes that Fluoresce upon Hybridization" (in en). Nature Biotechnology 14 (3): 303–308. doi:10.1038/nbt0396-303. ISSN 1546-1696. PMID 9630890. https://www.nature.com/articles/nbt0396-303. Retrieved 30 August 2022.
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