Chemistry:Dische test
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Short description: Chemical test
The Dische test, or Dische reaction, is used to distinguish DNA from RNA. It was invented by Zacharias Dische. It is a type of nitrate test.
Method
Dische's diphenylamine reagent consists of diphenylamine, glacial acetic acid, sulfuric acid, and ethanol.[1]
When heated with DNA, it turns blue in the presence of DNA. A more intense blue color indicates a greater concentration of DNA.
Mechanism
The acid converts deoxyribose to a molecule that binds with diphenylamine to form a blue substance. The reagent does not interact with RNA, so can be used to distinguish DNA from RNA.[2][3]
See also
References
- ↑ Patterson, J; Mura, C (4 February 2013). "Rapid colorimetric assays to qualitatively distinguish RNA and DNA in biomolecular samples.". Journal of Visualized Experiments (72): e50225. doi:10.3791/50225. PMID 23407542.
- ↑ "Nucleic Acids: DNA extraction and Dische's Diphenylamine test (Activity) | Biology OER". https://openlab.citytech.cuny.edu/bio-oer/chemistry/biologically-important-macromolecules/nucleic-acids/nucleic-acids-dna-extraction-and-disches-diphenylamine-test-activity/.
- ↑ "DNA-Dische Diphenylamine Determination". https://biocyclopedia.com/index/biotechnology_methods/molecular_biology/dna_dische_diphenylamine_determination.php.
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