Chemistry:Gallocyanin stain
The gallocyanin stain, also known as the gallocyanin-chromalum stain, is a stain of the oxazine group for total nucleic acids. It is prepared from gallocyanin and is an ideal method for numerous slides that need to be stained serially, equivalently, and reproducible.[1]
Structures containing basophilic compounds take on a bluish color.[1]
History
It has been known since the early work of Einarson (1932) that the gallocyanin dye worked well for nucleotide constituents.[2] Gersch and colleagues at Chicago are often credited with the earliest efforts of using gallocyanin for staining.
Sandritter demonstrated that a stoichiometric relationship occurs between intensity of staining and quantity of nucleic acid present.[3]
Function
Its method of binding and specificity are still not completely known. However, it is thought that gallocyanin-Cr(H2O)4 selectively binds to nucleic acid phosphate groups, particularly within a pH range of 1.5-1.75.[2]
References
- ↑ 1.0 1.1 C.K. Shah (1 January 1991). Modern Dictionary Cyto And Histo Chemistry. Discovery Publishing House. pp. 33–. ISBN 978-81-7141-151-1. https://books.google.com/books?id=IUPGQva4TLMC&pg=PA33. Retrieved 1 January 2013.
- ↑ 2.0 2.1 International review of cytology. 44.1976. Academic Press. 1976. pp. 150–. ISBN 978-0-12-364344-5. https://books.google.com/books?id=7KAql81UfAkC&pg=PA150. Retrieved 1 January 2013.
- ↑ Gérard Morel (1995). Visualization of Nucleic Acids. CRC Press. pp. 9–. ISBN 978-0-8493-4781-8. https://books.google.com/books?id=AsqPg940ImIC&pg=PA9. Retrieved 1 January 2013.
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