Chemistry:GelRed
From HandWiki
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| Preferred IUPAC name
5,5′-(6,22-Dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis(3,8-diamino-6-phenylphenanthridin-5-ium) diiodide | |
| Other names | |
| Identifiers | |
3D model (JSmol)
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PubChem CID
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| Properties | |
| C60H72I2N8O5 | |
| Molar mass | 1239.07 g/mol |
| Hazards | |
| Safety data sheet | 10,000X in water, Biotium Inc. |
| NFPA 704 (fire diamond) | |
| Flash point | > 100 °C (212 °F; 373 K) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
GelRed is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelRed structurally consists of two ethidium subunits that are bridged by a linear oxygenated spacer.[1][2]
GelRed is a fluorophore, and its optical properties are essentially identical to those of ethidium bromide. When exposed to ultraviolet light, it fluoresces with an orange color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelRed is sold as a solution in anhydrous DMSO or ultrapurified water.[3] GelRed is unable to cross cell membranes.[4]
See also
Short description: DNA gel stain for molecular genetics
References
- ↑ 1.0 1.1 1.2 & Leung, Wai-Yee"Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology" US patent application 2010323453
- ↑ GelRed & GelGreen, Biotium Inc., August 21, 2012, https://biotium.com/wp-content/uploads/2015/12/GelRed-GelGreen-PAGE-Gel-Stains-flyer.pdf, retrieved December 4, 2012
- ↑ 3.0 3.1 3.2 GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr, Biotium Inc., https://biotium.com/technology/gelred-gelgreen-nucleic-acid-gel-stains/, retrieved December 4, 2012
- ↑ Carson, Susan; Miller, Heather B.; Witherow, D. Scott (2012-01-01). "Lab Session 3 - PCR Amplification of egfp and Completion of Vector Preparation" (in en). Molecular Biology Techniques (3rd ed.). Academic Press. pp. 21–29. doi:10.1016/B978-0-12-385544-2.00003-X. ISBN 978-0-12-385544-2. https://www.sciencedirect.com/science/article/pii/B978012385544200003X.
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