Chemistry:SYTOX
From HandWiki
SYTOX (also known as SYTOX Green) is a high-affinity nucleic acid stain developed by biotechnology company Molecular Probes. Because the stain only penetrates cells with compromised plasma membranes, it can be used to investigate antibacterial mechanism of action[1] and confirm loss of bacterial viability.[2][3] There have, however, been studies which confirm the use of SYTOX dyes for live cell imaging of bacteria.[4]
References
- ↑ "Glochidioboside Kills Pathogenic Bacteria by Membrane Perturbation". Current Microbiology 71 (1): 1–7. July 2015. doi:10.1007/s00284-015-0807-9. PMID 25820208.
- ↑ "Use of SYTOX green dye in the flow cytometric analysis of bacterial phagocytosis". Cytometry 48 (2): 93–96. June 2002. doi:10.1002/cyto.10107. PMID 12116370.
- ↑ "Effectiveness of SYTOX Green stain for bacterial viability assessment". Applied and Environmental Microbiology 64 (7): 2697–2700. July 1998. doi:10.1128/AEM.64.7.2697-2700.1998. PMID 9647851.
- ↑ "Nonperturbative imaging of nucleoid morphology in live bacterial cells during an antimicrobial peptide attack". Applied and Environmental Microbiology 80 (16): 4977–4986. August 2014. doi:10.1128/AEM.00989-14. PMID 24907320.
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