Chemistry:Y-27632
Y-27632 is a biochemical tool used in the study of the rho-associated protein kinase (ROCK) signaling pathways.[1] Y-27632 selectively inhibits p160ROCK, although it does inhibit other protein kinases such as PKCs at higher concentrations.[2]
It has been studied for its effects on corneal endothelial cells (CECs) [3] and cardiac stem cells (CSCs).[4]
The substance has been used as part of a chemical cocktail to turn old and senescent human cells back into young ones (as measured by transcriptomic age), without turning them all the way back into undifferentiated stem cells.[5]
A mixture of methylcellulose (1%), ethylene glycol (10%), dimethyl sulfoxide (10%), and Y-27632 (10 μM), termed MEDY, has been shown to be an effective cryopreservation reagent for brain tissue, enabling tissue to resume growth and function after freezing using liquid nitrogen.[6]
References
- ↑ Uehata, Masayoshi (1999). "Y-27632. Selective probe of ROCK/Rho-kinase". Jikken Igaku 17 (7): 850–855.
- ↑ Uehata, M; Ishizaki, T; Satoh, H; Ono, T; Kawahara, T; Morishita, T; Tamakawa, H; Yamagami, K et al. (1997). "Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension". Nature 389 (6654): 990–4. doi:10.1038/40187. PMID 9353125. Bibcode: 1997Natur.389..990U.
- ↑ Peh, Gary S. L.; Adnan, Khadijah; George, Benjamin L.; Ang, Heng-Pei; Seah, Xin-Yi; Tan, Donald T.; Mehta, Jodhbir S. (2015-03-16). "The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach". Scientific Reports 5. doi:10.1038/srep09167. ISSN 2045-2322. PMID 25823914. Bibcode: 2015NatSR...5.9167P.
- ↑ Kan, Lijuan; Smith, Aubrie; Chen, Miao; Ledford, Benjamin T.; Fan, Huimin; Liu, Zhongmin; He, Jia-Qiang (2015-12-08). "Rho-Associated Kinase Inhibitor (Y-27632) Attenuates Doxorubicin-Induced Apoptosis of Human Cardiac Stem Cells". PLOS ONE 10 (12). doi:10.1371/journal.pone.0144513. ISSN 1932-6203. PMID 26645568. Bibcode: 2015PLoSO..1044513K.
- ↑ Yang, Jae-Hyun; Petty, Christopher A.; Dixon-McDougall, Thomas; Lopez, Maria Vina; Tyshkovskiy, Alexander; Maybury-Lewis, Sun; Tian, Xiao; Ibrahim, Nabilah et al. (2023-07-12). "Chemically induced reprogramming to reverse cellular aging" (in en). Aging 15 (13): 5966–5989. doi:10.18632/aging.204896. ISSN 1945-4589. PMID 37437248. PMC 10373966. https://www.aging-us.com/article/204896/text.
- ↑ Xue, Weiwei; Li, Huijuan; Xu, Jinhong; Yu, Xiao; Liu, Linlin; Liu, Huihui; Zhao, Rui; Shao, Zhicheng (2024). "Effective cryopreservation of human brain tissue and neural organoids". Cell Reports Methods 4 (5). doi:10.1016/j.crmeth.2024.100777. PMID 38744289.
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