Medicine:Turk's solution
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Short description: WBC diluting fluid
In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial)[1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent), and stains the nuclei of the white blood cells, making them easier to see and count.[1]
Türk's solution is intended for use in determining total leukocyte count in a defined volume of blood. Erythrocytes are hemolyzed while leukocytes are stained for easy visualization.[1][2]
Composition of Türk's solution is as follows:
| Component | Volume |
|---|---|
| Crystal violet | 1 mL[1] |
| Glacial acetic acid | 1.5 mL[1] |
| Distilled water | 100 mL[1] |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Chandrasekar, M. (December 2011) (in en). Practical Physiology Book. JP Medical Ltd. p. 27. ISBN 978-93-5025-513-1. OCLC 760979182. https://books.google.com/books?id=KNpN_jvbmAIC&dq=total_leukocyte_count&pg=PA27. Retrieved 28 February 2022.
- ↑ "Türk's solution 109277". https://www.sigmaaldrich.com/catalog/product/mm/109277. Retrieved 23 April 2019.
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