Medicine:Turk's solution

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Türk's solution in a laboratory bottle
Short description: WBC diluting fluid

In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial)[1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent), and stains the nuclei of the white blood cells, making them easier to see and count.[1]

Türk's solution is intended for use in determining total leukocyte count in a defined volume of blood. Erythrocytes are hemolyzed while leukocytes are stained for easy visualization.[1][2]

Composition of Türk's solution is as follows:

Component Volume
Crystal violet 1 mL[1]
Glacial acetic acid 1.5 mL[1]
Distilled water 100 mL[1]

References