Chemistry:Methylene blue

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Short description: Blue dye also used as a medication
Methylene blue
Methylene blue-2d-skeletal.svg
Molecular structure
Reflections in a flask of Methylene Blue.jpg
A volumetric flask of a methylene blue solution
Clinical data
Trade namesUrelene blue, Provayblue, Proveblue, others[1][2]
Other namesCI 52015, basic blue 9[3]
AHFS/Drugs.comMonograph
License data
Pregnancy
category
  • AU: D
Routes of
administration		By mouth, intravenous
ATC code
Legal status
Legal status
Pharmacokinetic data
Elimination half-life5 to 24 hours[5]
Identifiers
CAS Number
PubChem CID
DrugBank
ChemSpider
UNII
KEGG
ChEBI
ChEMBL
Chemical and physical data
FormulaC16H18ClN3S
Molar mass319.85 g·mol−1
3D model (JSmol)
  (verify)

Methylthioninium chloride, commonly called methylene blue, is a salt used as a dye and as a medication. As a medication, it is mainly used to treat methemoglobinemia by chemically reducing the ferric iron in hemoglobin to ferrous iron.[5][2] Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy.[2] It has previously been used for treating cyanide poisoning and urinary tract infections, but this use is no longer recommended.[5]

Methylene blue is typically given by injection into a vein.[5] Common side effects include headache and vomiting. While use during pregnancy may harm the baby, not using it in methemoglobinemia is likely more dangerous.[5][2]

Methylene blue was first prepared in 1876, by Heinrich Caro.[8] It is on the World Health Organization's List of Essential Medicines.[9]

Uses

Methemoglobinemia

Methylene blue is employed as a medication for the treatment of methemoglobinemia, which can arise from ingestion of certain pharmaceuticals, toxins, or broad beans in those susceptible.[10] Normally, through the NADH- or NADPH-dependent methemoglobin reductase enzymes, methemoglobin is reduced back to hemoglobin. When large amounts of methemoglobin occur secondary to toxins, methemoglobin reductases are overwhelmed. Methylene blue, when injected intravenously as an antidote, is itself first reduced to leucomethylene blue, which then reduces the heme group from methemoglobin to hemoglobin. Methylene blue can reduce the half life of methemoglobin from hours to minutes.[11] At high doses, however, methylene blue actually induces methemoglobinemia, reversing this pathway.[11]

Methylphen

Cyanide poisoning

Since its reduction potential is similar to that of oxygen and can be reduced by components of the electron transport chain, large doses of methylene blue are sometimes used as an antidote to potassium cyanide poisoning, a method first successfully tested in 1933 by Dr. Matilda Moldenhauer Brooks in San Francisco,[12] although first demonstrated by Bo Sahlin of Lund University, in 1926.[12][13]

Dye or stain

Human cheek cells stained with methylene blue
Methylene blue crystals

Methylene blue is used in endoscopic polypectomy as an adjunct to saline or epinephrine, and is used for injection into the submucosa around the polyp to be removed. This allows the submucosal tissue plane to be identified after the polyp is removed, which is useful in determining if more tissue needs to be removed, or if there has been a high risk for perforation. Methylene blue is also used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions. Intravenously injected methylene blue is readily released into the urine and thus can be used to test the urinary tract for leaks or fistulas.[citation needed]

In surgeries such as sentinel lymph node dissections, methylene blue can be used to visually trace the lymphatic drainage of tested tissues. Similarly, methylene blue is added to bone cement in orthopedic operations to provide easy discrimination between native bone and cement. Additionally, methylene blue accelerates the hardening of bone cement, increasing the speed at which bone cement can be effectively applied. Methylene blue is used as an aid to visualisation/orientation in a number of medical devices, including a Surgical sealant film, TissuePatch. In fistulas and pilonidal sinuses it is used to identify the tract for complete excision.[citation needed] It can also be used during gastrointestinal surgeries (such as bowel resection or gastric bypass) to test for leaks.[citation needed]

It is sometimes used in cytopathology, in mixtures including Wright-Giemsa and Diff-Quik. It confers a blue color to both nuclei and cytoplasm, and makes the nuclei more visible.[14] When methylene blue is "polychromed" (oxidized in solution or "ripened" by fungal metabolism,[15] as originally noted in the thesis of Dr. D. L. Romanowsky in the 1890s), it gets serially demethylated and forms all the tri-, di-, mono- and non-methyl intermediates, which are Azure B, Azure A, Azure C, and thionine, respectively.[16] This is the basis of the basophilic part of the spectrum of Romanowski-Giemsa effect. If only synthetic Azure B and Eosin Y is used, it may serve as a standardized Giemsa stain; but, without methylene blue, the normal neutrophilic granules tend to overstain and look like toxic granules. On the other hand, if methylene blue is used it might help to give the normal look of neutrophil granules and may also enhance the staining of nucleoli and polychromatophilic RBCs (reticulocytes).[17]

A traditional application of methylene blue is the intravital or supravital staining of nerve fibers, an effect first described by Paul Ehrlich in 1887.[18] A dilute solution of the dye is either injected into tissue or applied to small freshly removed pieces. The selective blue coloration develops with exposure to air (oxygen) and can be fixed by immersion of the stained specimen in an aqueous solution of ammonium molybdate. Vital methylene blue was formerly much used for examining the innervation of muscle, skin and internal organs.[19][20][21] The mechanism of selective dye uptake is incompletely understood; vital staining of nerve fibers in skin is prevented by ouabain, a drug that inhibits the Na/K-ATPase of cell membranes.[22]

Placebo

Methylene blue has been used as a placebo; physicians would tell their patients to expect their urine to change color and view this as a sign that their condition had improved.[23] This same side effect makes methylene blue difficult to use in traditional placebo-controlled clinical studies, including those testing for its efficacy as a treatment.[24]

Isobutyl nitrite toxicity

Isobutyl nitrite is one of the compounds used as poppers, an inhalant drug that induces a brief euphoria.

Isobutyl nitrite is known to cause methemoglobinemia.[25] Severe methemoglobinemia may be treated with methylene blue.[26]

Gross pathology of a normal brain and a brain of a patient treated with methylene blue before death.

Ifosfamide toxicity

Another use of methylene blue is to treat ifosfamide neurotoxicity. Methylene blue was first reported for treatment and prophylaxis of ifosfamide neuropsychiatric toxicity in 1994. A toxic metabolite of ifosfamide, chloroacetaldehyde (CAA), disrupts the mitochondrial respiratory chain, leading to an accumulation of nicotinamide adenine dinucleotide hydrogen (NADH). Methylene blue acts as an alternative electron acceptor, and reverses the NADH inhibition of hepatic gluconeogenesis while also inhibiting the transformation of chloroethylamine into chloroacetaldehyde, and inhibits multiple amine oxidase activities, preventing the formation of CAA.[27] The dosing of methylene blue for treatment of ifosfamide neurotoxicity varies, depending upon its use simultaneously as an adjuvant in ifosfamide infusion, versus its use to reverse psychiatric symptoms that manifest after completion of an ifosfamide infusion. Reports suggest that methylene blue up to six doses a day have resulted in improvement of symptoms within 10 minutes to several days.[28] Alternatively, it has been suggested that intravenous methylene blue every six hours for prophylaxis during ifosfamide treatment in people with history of ifosfamide neuropsychiatric toxicity.[29] Prophylactic administration of methylene blue the day before initiation of ifosfamide, and three times daily during ifosfamide chemotherapy has been recommended to lower the occurrence of ifosfamide neurotoxicity.[30]

Shock

It has also been used in septic shock and anaphylaxis.[31][32]

Methylene blue consistently increases blood pressure in people with vasoplegic syndrome (redistributive shock), but has not been shown to improve delivery of oxygen to tissues or to decrease mortality.[33][34][35][36]

Methylene blue has been used in calcium channel blocker toxicity as a rescue therapy for distributive shock unresponsive to first line agents. Evidence for its use in this circumstance is very poor and limited to a handful of case reports.[37][38][39][40][41][42][43][44][45][excessive citations]

Side effects

Cardiovascular[46][47] Central Nervous System[46][47] Dermatologic[46][47] Gastrointestinal[46][47] Genito-urinary[46][47] Hematologic[46][47]
• Hypertension
• Precordial pain 		• Dizziness
• Mental confusion
• Headache
• Fever 		• Staining of skin
• Injection site necrosis (SC) 		• Fecal discoloration
• Nausea
• Vomiting
• Abdominal pain 		• Discoloration of urine (doses over 80 µg)
• Bladder irritation 		• Anemia

Methylene blue is a monoamine oxidase inhibitor (MAOI),[48] and if infused intravenously at doses exceeding 5 mg/kg, may precipitate serious serotonin toxicity, serotonin syndrome, if combined with any selective serotonin reuptake inhibitors (SSRIs) or other serotonin reuptake inhibitor (e.g., duloxetine, sibutramine, venlafaxine, clomipramine, imipramine).[49]

It causes hemolytic anemia in carriers of the G6PD (favism) enzymatic deficiency.[50]

Chemistry

Methylene blue is a formal derivative of phenothiazine. It is a dark green powder that yields a blue solution in water. The hydrated form has 3 molecules of water per unit of methylene blue.

Preparation

This compound is prepared by oxidation of 4-aminodimethylaniline in the presence of sodium thiosulfate to give the quinonediiminothiosulfonic acid, reaction with dimethylaniline, oxidation to the indamine, and cyclization to give the thiazine:[51]

Synthesis of methylene blue.png

A green electrochemical procedure, using only dimethyl-4-phenylenediamine and sulfide ions has been proposed.[52]

Light absorption properties

Absorption spectrum of methylene blue, in terms of the molar extinction coefficient (base 10 logarithm). In this dataset a peak absorbance of 1.7 (i.e. 98% of transmitted light absorbed) was observed with 665 nm light passing through 1 cm of 10 micromolar methylene blue solution.

The maximum absorption of light is near 670 nm. The specifics of absorption depend on a number of factors, including protonation, adsorption to other materials, and metachromasy - the formation of dimers and higher-order aggregates depending on concentration and other interactions:[53]

Species Absorption peak Extinction coefficient (dm3/mole·cm)
MB+ (solution) 664 95000
MBH2+ (solution) 741 76000
(MB+)2 (solution) 605 132000
(MB+)3 (solution) 580 110000
MB+ (adsorbed on clay) 673 116000
MBH2+ (adsorbed on clay) 763 86000
(MB+)2 (adsorbed on clay) 596 80000
(MB+)3 (adsorbed on clay) 570 114000

Other uses

Redox indicator

Methylene blue is widely used as a redox indicator in analytical chemistry.[54][55][56] Solutions of this substance are blue when in an oxidizing environment, but will turn colorless if exposed to a reducing agent.[57][55] The redox properties can be seen in a classical demonstration of chemical kinetics in general chemistry, the "blue bottle" experiment. Typically, a solution is made of glucose (dextrose), methylene blue, and sodium hydroxide. Upon shaking the bottle, oxygen oxidizes methylene blue, and the solution turns blue. The dextrose will gradually reduce the methylene blue to its colorless, reduced form. Hence, when the dissolved dextrose is entirely consumed, the solution will turn blue again. The redox midpoint potential E0' is +0.01 V.[58]

Peroxide generator

Methylene blue is also a photosensitizer used to create singlet oxygen when exposed to both oxygen and light. It is used in this regard to make organic peroxides by a Diels-Alder reaction which is spin forbidden with normal atmospheric triplet oxygen.[citation needed]

Sulfide analysis

The formation of methylene blue after the reaction of hydrogen sulfide with dimethyl-p-phenylenediamine and iron(III) at pH 0.4 – 0.7 is used to determine by photometric measurements sulfide concentration in the range 0.020 to 1.50 mg/L (20 ppb to 1.5 ppm).[59] The test is very sensitive and the blue coloration developing upon contact of the reagents with dissolved H2S is stable for 60 min. Ready-to-use kits such as the Spectroquant sulfide test[60] facilitate routine analyses. The methylene blue sulfide test is a convenient method often used in soil microbiology to quickly detect in water the metabolic activity of sulfate reducing bacteria (SRB). It must be noted that in this colorimetric test, methylene blue is a product formed by the reaction and not a reagent added to the system.[59]

The addition of a strong reducing agent, such as ascorbic acid, to a sulfide-containing solution is sometimes used to prevent sulfide oxidation from atmospheric oxygen. Although it is certainly a sound precaution for the determination of sulfide with an ion selective electrode, it might however hamper the development of the blue color if the freshly formed methylene blue is also reduced, as described here above in the paragraph on redox indicator.[55]

Test for milk freshness

Methylene blue is a dye behaving as a redox indicator that is commonly used in the food industry to test the freshness of milk and dairy products.[5][61] A few drops of methylene blue solution added to a sample of milk should remain blue (oxidized form in the presence of enough dissolved O2), otherwise (discoloration caused by the reduction of methylene blue into its colorless reduced form) the dissolved O2 concentration in the milk sample is low indicating that the milk is not fresh (already abiotically oxidized by O2 whose concentration in solution decreases) or could be contaminated by bacteria also consuming the atmospheric O2 dissolved in the milk.[61] In other words, aerobic conditions should prevail in fresh milk and methylene blue is simply used as an indicator of the dissolved oxygen remaining in the milk.[57]

Water testing

Further information: Chemistry:MBAS assay

The adsorption of methylene blue serves as an indicator defining the adsorptive capacity of granular activated carbon in water filters. Adsorption of methylene blue is very similar to adsorption of pesticides from water, this quality makes methylene blue serve as a good predictor for filtration qualities of carbon. It is as well a quick method of comparing different batches of activated carbon of the same quality. A color reaction in an acidified, aqueous methylene blue solution containing chloroform can detect anionic surfactants in a water sample. Such a test is known as an MBAS assay (methylene blue active substances assay).

The MBAS assay cannot distinguish between specific surfactants, however. Some examples of anionic surfactants are carboxylates, phosphates, sulfates, and sulfonates.[citation needed]

Methylene blue value of fine aggregate

The methylene blue value is defined as the number of milliliter's standard methylene value solution decolorized 0.1 g of activated carbon (dry basis).[62] Methylene blue value reflects the amount of clay minerals in aggregate samples.[63] In materials science, methylene blue solution is successively added to fine aggregate which is being agitated in water. The presence of free dye solution can be checked with stain test on a filter paper.[64]

Biological staining

In biology, methylene blue is used as a dye for a number of different staining procedures, such as Wright's stain and Jenner's stain. Since it is a temporary staining technique, methylene blue can also be used to examine RNA or DNA under the microscope or in a gel: as an example, a solution of methylene blue can be used to stain RNA on hybridization membranes in northern blotting to verify the amount of nucleic acid present. While methylene blue is not as sensitive as ethidium bromide, it is less toxic and it does not intercalate in nucleic acid chains, thus avoiding interference with nucleic acid retention on hybridization membranes or with the hybridization process itself.[citation needed]

It can also be used as an indicator to determine whether eukaryotic cells such as yeast are alive or dead. The methylene blue is reduced in viable cells, leaving them unstained. However dead cells are unable to reduce the oxidized methylene blue and the cells are stained blue. Methylene blue can interfere with the respiration of the yeast as it picks up hydrogen ions made during the process.[citation needed]

Aquaculture

Methylene blue is used in aquaculture and by tropical fish hobbyists as a treatment for fungal infections. It can also be effective in treating fish infected with ich although a combination of malachite green and formaldehyde is far more effective against the parasitic protozoa Ichthyophthirius multifiliis. It is usually used to protect newly laid fish eggs from being infected by fungus or bacteria. This is useful when the hobbyist wants to artificially hatch the fish eggs. Methylene blue is also very effective when used as part of a "medicated fish bath" for treatment of ammonia, nitrite, and cyanide poisoning as well as for topical and internal treatment of injured or sick fish as a "first response".[65]

History

Methylene blue has been described as "the first fully synthetic drug used in medicine." Methylene blue was first prepared in 1876 by German chemist Heinrich Caro.[66]

Its use in the treatment of malaria was pioneered by Paul Guttmann and Paul Ehrlich in 1891. During this period before the first World War, researchers like Ehrlich believed that drugs and dyes worked in the same way, by preferentially staining pathogens and possibly harming them. Changing the cell membrane of pathogens is in fact how various drugs work, so the theory was partially correct although far from complete. Methylene blue continued to be used in the second World War, where it was not well liked by soldiers, who observed, "Even at the loo, we see, we pee, navy blue." Antimalarial use of the drug has recently been revived.[67] It was discovered to be an antidote to carbon monoxide poisoning and cyanide poisoning in 1933 by Matilda Brooks.[68]

The blue urine was used to monitor psychiatric patients' compliance with medication regimes. This led to interest - from the 1890s to the present day - in the drug's antidepressant and other psychotropic effects. It became the lead compound in research leading to the discovery of chlorpromazine.[69]

Names

The International Nonproprietary Name (INN) of methylene blue is methylthioninium chloride.[70][71]

Research

Malaria

Methylene blue was identified by Paul Ehrlich about 1891 as a possible treatment for malaria.[72] It disappeared as an anti-malarial during the Pacific War in the tropics, since American and Allied soldiers disliked its two prominent, but reversible side effects: turning the urine blue or green, and the sclera (the whites of the eyes) blue. Interest in its use as an anti-malarial has recently been revived,[69] especially due to its low price. Several clinical trials are in progress, trying to find a suitable drug combination. According to studies on children in Africa, it appears to have efficacy against malaria, but the attempts to combine methylene blue with chloroquine were disappointing.[73]

Alzheimer's

A Phase 3 clinical trial of LMTM (TauRx0237 or LMT-X), a derivative of methylene blue, failed to show any benefit against cognitive or functional decline in people with mild to moderate Alzheimer's disease. Disease progression for both the drug and the placebo were practically identical.[74][75]

Bipolar disorder

Methylene blue has been studied as an adjunctive medication in the treatment of bipolar disorder.[76]

Infectious diseases

It has been studied in AIDS-related Kaposi's sarcoma,[77] West Nile virus,[78] and to inactivate Staphylococcus aureus,[79] and HIV-1.[80] Phenothiazine dyes and light have been known to have virucidal properties for over 70 years.[81]

References

  1. ↑ Tarascon Pocket Pharmacopoeia 2015 Deluxe Lab-Coat Edition. Jones & Bartlett Learning. 2015. p. 471. ISBN 9781284057560. 
  2. ↑ 2.0 2.1 2.2 2.3 British national formulary : BNF 69 (69 ed.). British Medical Association. 2015. p. 34. ISBN 9780857111562. 
  3. ↑ H. J. Conn's Biological stains (9th ed.). Baltimore: Williams & Wilkins. 1977. pp. 692p. 
  4. ↑ "Provayblue- methylene blue injection". 29 June 2022. https://dailymed.nlm.nih.gov/dailymed/drugInfo.cfm?setid=4f6848e5-35ed-4046-b13c-3032b5ba3232. 
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 "Methylene Blue". The American Society of Health-System Pharmacists. https://www.drugs.com/monograph/methylene-blue.html. 
  6. ↑ "Lumeblue EPAR". 19 June 2020. https://www.ema.europa.eu/en/medicines/human/EPAR/lumeblue. 
  7. ↑ "Lumeblue Product information". https://ec.europa.eu/health/documents/community-register/html/h1470.htm. 
  8. ↑ (in en) New Strategies Combating Bacterial Infection. John Wiley & Sons. 2008. p. 91. ISBN 9783527622948. https://books.google.com/books?id=Hrdzorisyp8C&pg=PA91. 
  9. ↑ World Health Organization model list of essential medicines: 21st list 2019. Geneva: World Health Organization. 2019. WHO/MVP/EMP/IAU/2019.06. License: CC BY-NC-SA 3.0 IGO. 
  10. ↑ "Hemolytic Anemia: Hereditary and Acquired". Manual of Clinical Hematology (3rd ed.). Philadelphia: Lippincott Williams & Wilkins. 2002. p. 102. ISBN 978-0-7817-2980-2. https://books.google.com/books?id=NzMKMPzbxZwC&pg=PA102. 
  11. ↑ 11.0 11.1 Brent J. (2005). Critical care toxicology: diagnosis and management of the critically poisoned patient.. Elsevier Health Sciences. 
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  15. ↑ "Chapter 19: On Chemical Reactions and Staining Mechanisms". Special Stains and H & E. Education Guide (Second ed.). Carpinteria, California: Dako North America, Inc.. 2010. p. 172. http://www.dako.com/08066_12may10_webchapter19.pdf. "What is Giemsa's stain and how does it color blood cells, bacteria and chromosomes?" 
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  26. ↑ "Methylene blue: a treatment for severe methaemoglobinaemia secondary to misuse of amyl nitrite". Emergency Medicine Journal 19 (3): 270–271. May 2002. doi:10.1136/emj.19.3.270. PMID 11971852. 
  27. ↑ "Ifosfamide neuropsychiatric toxicity in patients with cancer". Psycho-Oncology 16 (10): 956–960. October 2007. doi:10.1002/pon.1161. PMID 17278152. 
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  29. ↑ "[Ifosfamide induced encephalopathy: 15 observations]" (in fr). Archives de Pédiatrie 13 (2): 140–145. February 2006. doi:10.1016/j.arcped.2005.10.021. PMID 16364615. 
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  32. ↑ "Methylene blue for the treatment of septic shock". Pharmacotherapy 30 (7): 702–715. July 2010. doi:10.1592/phco.30.7.702. PMID 20575634. 
  33. ↑ "Methylene Blue: Magic Bullet for Vasoplegia?". Anesthesia and Analgesia 122 (1): 194–201. January 2016. doi:10.1213/ANE.0000000000001045. PMID 26678471. 
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  39. ↑ "Methylene blue reverses recalcitrant shock in β-blocker and calcium channel blocker overdose". BMJ Case Reports 2013: bcr2012007402. January 2013. doi:10.1136/bcr-2012-007402. PMID 23334490. 
  40. ↑ ""Feeling the Blues": A Case of Calcium Channel Blocker Overdose Managed With Methylene Blue". Cureus 13 (10): e19114. October 2021. doi:10.7759/cureus.19114. PMID 34868762. 
  41. ↑ "Hemodynamic improvement using methylene blue after calcium channel blocker overdose". World Journal of Emergency Medicine 10 (1): 55–58. 2019. doi:10.5847/wjem.j.1920-8642.2019.01.009. PMID 30598720. 
  42. ↑ "A multifaceted approach to calcium channel blocker overdose: a case report and literature review". Clinical Case Reports 3 (7): 566–569. July 2015. doi:10.1002/ccr3.300. PMID 26273444. 
  43. ↑ "A Case of Severe, Refractory Hypotension After Amlodipine Overdose". Cardiovascular Toxicology 18 (2): 192–197. April 2018. doi:10.1007/s12012-017-9419-x. PMID 28688059. 
  44. ↑ "Methylene blue in the treatment of refractory shock from an amlodipine overdose". Annals of Emergency Medicine 58 (6): 565–567. December 2011. doi:10.1016/j.annemergmed.2011.02.025. PMID 21546119. 
  45. ↑ "Improvement in Hemodynamics After Methylene Blue Administration in Drug-Induced Vasodilatory Shock: A Case Report". Journal of Medical Toxicology 11 (4): 460–463. December 2015. doi:10.1007/s13181-015-0500-1. PMID 26310944. 
  46. ↑ 46.0 46.1 46.2 46.3 46.4 46.5 "Adult toxicology in critical care: Part II: specific poisonings". Chest 123 (3): 897–922. March 2003. doi:10.1378/chest.123.3.897. PMID 12628894. https://journal.chestnet.org/article/S0012-3692(16)34761-4/fulltext. 
  47. ↑ 47.0 47.1 47.2 47.3 47.4 47.5 "Studies of the efficacy and potential hazards of methylene blue therapy in aniline-induced methaemoglobinaemia". British Journal of Haematology 54 (1): 29–41. May 1983. doi:10.1111/j.1365-2141.1983.tb02064.x. PMID 6849836. https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2141.1983.tb02064.x. 
  48. ↑ "Methylene blue and serotonin toxicity: inhibition of monoamine oxidase A (MAO A) confirms a theoretical prediction". British Journal of Pharmacology 152 (6): 946–951. November 2007. doi:10.1038/sj.bjp.0707430. PMID 17721552. 
  49. ↑ "Methylene blue implicated in potentially fatal serotonin toxicity". Anaesthesia 61 (10): 1013–1014. October 2006. doi:10.1111/j.1365-2044.2006.04808.x. PMID 16978328. 
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  51. ↑ Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. 2012. doi:10.1002/14356007.a03_213.pub3. ISBN 978-3527306732. 
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