Physics:EM Data Bank
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| Content | |
| Description | Unified Data Resource for CryoEM. |
| Contact | |
| Research centre | European Bioinformatics Institute (UK site) AND Rutgers University (USA site) |
| Laboratory | PDBe & RCSB PDB |
| Primary citation | Lawson & al. (2011)[1] |
| Release date | 2002 |
| Access | |
| Website | ebi |
The EM Data Bank or Electron Microscopy Data Bank (EMDB) collects 3D EM maps and associated experimental data determined using electron microscopy of biological specimens. It was established in 2002 at the MSD/PDBe group of the European Bioinformatics Institute (EBI), where the European site of the EMDataBank.org consortium is located.[2] (As of 2015), the resource contained over 2,600 entries with a mean resolution of 15Å.[3]
Deposition of data was originally via the EMdep deposition interface, but since 2016 deposition of data has been incorporated into the wwPDB OneDep interface.[4]
Under the NIH Unified Data Resource for CryoEM, the Research Collaboration for Structural Biology (RCSB) also acts as a deposition, data processing and distribution center for EMDB data, while the National Center for Macromolecular Imaging (NCMI) is a collaborative partner in providing services and tools concerning the EMDB.
EM Data Bank also provides the EMsearch search tool and data can also be queried at RCSB, EMBL-EBI and PDBj.
EMDB is an archive for three-dimensional density maps of all types of biological assemblies, including ribosomes, chaperones, polymerases, multifunctional enzymes and viruses. Viper EMDB at Scripps is a separate database for three-dimensional EM maps of viruses.
To compare and assess methods for the new generation of higher resolution (better than 5Å) structures, the EMDB has hosted the first CryoEM Map Challenge and CryoEM Model Challenge, reported in a special issue of the Journal of Structural Biology.[5]
See also
- Cryo-Electron Microscopy
- Colocalization Benchmark Source
References
- ↑ Lawson, Catherine L; Baker, Matthew L; Best, Christoph et al. (January 2011). "EMDataBank.org: unified data resource for CryoEM". Nucleic Acids Research 39 (suppl 1): D456-464. doi:10.1093/nar/gkq880. PMID 20935055.
- ↑ EMDB. "Electron Microscopy Data Bank" (in en). https://www.ebi.ac.uk/emdb/about.
- ↑ Esquivel-Rodríguez, J; Xiong, Y; Han, X et al. (30 May 2015). "Navigating 3D electron microscopy maps with EM-SURFER". BMC Bioinform. 16 (181): 181. doi:10.1186/s12859-015-0580-6. PMID 26025554.
- ↑ Young, JY; Westbrook, JD; Feng, Z; Sala, R; Peisach, E; Oldfield, TJ; Sen, S; Gutmanas, A et al. (2017). "OneDep: Unified wwPDB System for Deposition, Biocuration, and Validation of Macromolecular Structures in the PDB Archive". Structure 25 (3): 536–545. doi:10.1016/j.str.2017.01.004. PMID 28190782.
- ↑ Lawson, Catherine; Chiu, Wah, eds (2018). "The CryoEM Structure Map and Model Challenges". Journal of Structural Biology 204 (1). ISSN 1047-8477. https://journals.elsevier.com/journal-of-structural-biology/thematic-collections/the-cryoem-structure-map-and-model-challenges.
- Tagari, Mohamed; Newman, Richard; Chagoyen, Monica; Carazo, Jose-Maria; Henrick, Kim (2002). "New electron microscopy database and deposition system". Trends in Biochemical Sciences 27 (11): 589. doi:10.1016/s0968-0004(02)02176-x. PMID 12417136.
- Fuller, Stephen D. (2003). "Depositing electron microscopy maps". Structure 11 (1): 11–12. doi:10.1016/s0969-2126(02)00942-5. PMID 12517335.
- Henrick, K; Newman, R; Tagari, M; Chagoyenb, M (2003). "EMDep: a web-based system for the deposition and validation of high-resolution electron microscopy macromolecular structural information". Journal of Structural Biology 144 (1–2): 228–237. doi:10.1016/j.jsb.2003.09.009. PMID 14643225.
- Heymann, J. Bernard; Chagoyen, Mónica; Belnap, David M. (2005). "Common conventions for interchange and archiving of three-dimensional electron microscopy information in structural biology". Journal of Structural Biology 151 (2): 196–207. doi:10.1016/j.jsb.2005.06.001. PMID 16043364.
- Tagari, M.; Tate, J.; Swaminathan, G. J. et al. (2006). "E-MSD: improving data deposition and structure quality". Nucleic Acids Research 34 (Database issue): 287–290. doi:10.1093/nar/gkj163. PMID 16381867.
External links
- Software tools for molecular microscopy
- EM Data Bank
- US and UK Search pages
- Japan Search page (EM Navigator in PDBj)
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