Physics:Endcapping
From HandWiki
In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns.[1] Endcapping technology prevents the tailing of a polar compound's peak and shows very high durability even with an alkaline mobile phase because of the strong film covering the stationary phase surface.
Endcapped columns show decreased retention for hydrogen bond acceptors, such as ionized bases, and increased retention for protonated bases.[2]
Carbon nano tubes (CNT) also can be used in end capping.
References
- ↑ "Comparison of the acidity of residual silanol groups in several liquid chromatography columns". Journal of Chromatography 986 (1): 33–44. 31 January 2003. doi:10.1016/S0021-9673(02)01899-X. PMID 12585320.
- ↑ "Column selectivity in reversed-phase liquid chromatography: VI. Columns with embedded or end-capping polar groups". Journal of Chromatography 1026 (1–2): 91–100. 13 February 2004. doi:10.1016/j.chroma.2003.11.041. PMID 14763736.
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