Biology:RprA RNA
| RprA RNA | |
|---|---|
 Predicted secondary structure and sequence conservation of RprA | |
| Identifiers | |
| Symbol | RprA |
| Rfam | RF00034 |
| Other data | |
| RNA type | Gene; sRNA |
| Domain(s) | Bacteria |
| SO | 0000387 |
| PDB structures | PDBe |
The RprA RNA gene encodes a 106 nucleotide regulatory non-coding RNA. Translational regulation of the stationary phase sigma factor RpoS is mediated by the formation of a double-stranded RNA stem-loop structure in the upstream region of the rpoS messenger RNA, occluding the translation initiation site.[1][2]
Clones carrying rprA (RpoS regulator RNA A) increased the translation of RpoS. As with DsrA, RprA is predicted to form three stem-loops. Thus, at least two small RNAs, DsrA and RprA, participate in the positive regulation of RpoS translation. RprA also appears to bind to the RpoS leader.[3] RprA is non-essential.[4] Wasserman et al. demonstrated that this RNA is bound by the Hfq protein.[5] Binding to Hfq alters the conformation of RprA.[6] In the presence of Hfq the stability of RprA is influenced by the osmolarity of the cell, this is dependent on the endoribonuclease RNase E.[7]
It has been shown the RprA regulates the protein coding genes, called csgD, this protein encodes a stationary phase-induced biofilm regulator and ydaM, which encodes a diguanylate cyclase involved in activating csgD transcription. These two target genes are repressed by RprA which results in regulation of biofilm formation.[8]
References
- ↑ "Effect of Hfq on RprA-rpoS mRNA pairing: Hfq-RNA binding and the influence of the 5′ rpoS mRNA leader region.". Biochemistry 47 (43): 11184–11195. 2008. doi:10.1021/bi800479p. PMID 18826256.
- ↑ "Limited Role for the DsrA and RprA Regulatory RNAs in rpoS Regulation in Salmonella enterica". J Bacteriol 188 (14): 5077–5088. 2006. doi:10.1128/JB.00206-06. PMID 16816180.
- ↑ Majdalani, N; Hernandez D; Gottesman S (2002). "Regulation and mode of action of the second small RNA activator of RpoS translation, RprA". Mol Microbiol 46 (3): 813–826. doi:10.1046/j.1365-2958.2002.03203.x. PMID 12410838.
- ↑ Majdalani, N; Chen S; Murrow J; St John K; Gottesman S (2001). "Regulation of RpoS by a novel small RNA: the characterization of RprA". Mol Microbiol 39 (5): 1382–1394. doi:10.1111/j.1365-2958.2001.02329.x. PMID 11251852.
- ↑ "Identification of novel small RNAs using comparative genomics and microarrays". Genes Dev. 15 (13): 1637–1651. 2001. doi:10.1101/gad.901001. PMID 11445539.
- ↑ "Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS.". RNA 19 (8): 1089–1104. August 2013. doi:10.1261/rna.034595.112. PMID 23804244.
- ↑ "Turn-over of the small non-coding RNA RprA in E. coli is influenced by osmolarity". Mol Genet Genomics 284 (4): 307–318. 2010. doi:10.1007/s00438-010-0568-x. PMID 20717695.
- ↑ "Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli.". Mol Microbiol 84 (1): 51–65. 2012. doi:10.1111/j.1365-2958.2012.08002.x. PMID 22356413.
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