Chemistry:GelGreen
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| Preferred IUPAC name
10,10′-(6,22-Dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis[3,6-bis(dimethylamino)acridin-10-ium] diiodide | |
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3D model (JSmol)
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PubChem CID
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| Properties | |
| C56H80I2N8O5 | |
| Molar mass | 1198.43 g/mol |
| Hazards | |
| Safety data sheet | 10,000X in water, Biotium Inc. |
| NFPA 704 (fire diamond) | |
| Flash point | > 100 °C (212 °F; 373 K) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
GelGreen is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelGreen consists of two acridine orange subunits that are bridged by a linear oxygenated spacer.[1][2]
Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ultraviolet light, it will fluoresce with a greenish color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelGreen is sold as a solution in either DMSO or water.[3]
Short description: DNA gel stain for molecular genetics
See also
- Ethidium bromide
- GelRed
- SYBR Green I
- Agarose gel electrophoresis and gel electrophoresis of nucleic acids
- Acridine orange
References
- ↑ 1.0 1.1 1.2 & Leung, Wai-Yee"Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology" US patent application 2010323453
- ↑ GelRed & GelGreen, Biotium Inc., August 21, 2012, https://biotium.com/wp-content/uploads/2015/12/GelRed-GelGreen-PAGE-Gel-Stains-flyer.pdf, retrieved December 4, 2012
- ↑ 3.0 3.1 3.2 GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr, Biotium Inc., https://biotium.com/technology/gelred-gelgreen-nucleic-acid-gel-stains/, retrieved December 4, 2012
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