Biology:POLI

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from Pol η. Pol ι, is a Y family polymerase that is involved in translesion synthesis. It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses Hoogsteen base pairing, during DNA synthesis, it will add adenine opposite to thymine in the syn conformation and can add both cytosine and thymine in the anti conformation across guanine, which it flips to the syn conformation.

Further reading

• "Polymerase iota - an odd sibling among Y family polymerases.". DNA Repair 86 (102753): 102753. 2020. doi:10.1016/j.dnarep.2019.102753. PMID 31805501. 
• "DNA polymerase iota and related rad30-like enzymes.". Philos. Trans. R. Soc. Lond. B Biol. Sci. 356 (1405): 53–60. 2001. doi:10.1098/rstb.2000.0748. PMID 11205331. 
• "Biochemical characterization of human DNA polymerase iota provides clues to its biological function.". Biochem. Soc. Trans. 29 (Pt 2): 183–7. 2001. doi:10.1042/BST0290183. PMID 11356150. 
• "Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.". Genomics 60 (1): 20–30. 1999. doi:10.1006/geno.1999.5906. PMID 10458907. https://zenodo.org/record/1229798. 
• "Mechanisms of accurate translesion synthesis by human DNA polymerase eta.". EMBO J. 19 (12): 3100–9. 2000. doi:10.1093/emboj/19.12.3100. PMID 10856253. 
• "poliota, a remarkably error-prone human DNA polymerase.". Genes Dev. 14 (13): 1642–50. 2000. doi:10.1101/gad.14.13.1642. PMID 10887158. 
• "Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota.". EMBO J. 19 (19): 5259–66. 2000. doi:10.1093/emboj/19.19.5259. PMID 11013228. 
• "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. 2001. doi:10.1101/gr.143000. PMID 11076863. 
• "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.". Genome Res. 11 (3): 422–35. 2001. doi:10.1101/gr.GR1547R. PMID 11230166. 
• "5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro.". Science 291 (5511): 2156–9. 2001. doi:10.1126/science.1058386. PMID 11251121. Bibcode: 2001Sci...291.2156B. https://zenodo.org/record/1230838. 
• "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.". EMBO Rep. 1 (3): 287–92. 2001. doi:10.1093/embo-reports/kvd058. PMID 11256614. 
• "Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template.". EMBO J. 20 (11): 2914–22. 2001. doi:10.1093/emboj/20.11.2914. PMID 11387224. 
• "Human DNA polymerase iota promiscuous mismatch extension.". J. Biol. Chem. 276 (33): 30615–22. 2001. doi:10.1074/jbc.M102694200. PMID 11402031. 
• "The Y-family of DNA polymerases.". Mol. Cell 8 (1): 7–8. 2001. doi:10.1016/S1097-2765(01)00278-7. PMID 11515498. 
• "Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines.". EMBO J. 20 (22): 6520–9. 2002. doi:10.1093/emboj/20.22.6520. PMID 11707422. 
• "Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota.". Nature 419 (6910): 944–7. 2002. doi:10.1038/nature01117. PMID 12410315. Bibcode: 2002Natur.419..944F. 
• "Localization of DNA polymerases eta and iota to the replication machinery is tightly co-ordinated in human cells.". EMBO J. 21 (22): 6246–56. 2003. doi:10.1093/emboj/cdf618. PMID 12426396. 
• "Translesion replication of benzo[apyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase iota."]. Nucleic Acids Res. 30 (23): 5284–92. 2002. doi:10.1093/nar/gkf643. PMID 12466554. 
• "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. 2003. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M.