Biology:STX8
From HandWiki
Short description: Protein-coding gene in the species Homo sapiens
 Generic protein structure example |
Syntaxin-8 is a protein that in humans is encoded by the STX8 gene.[1][2][3] Syntaxin 8 directly interacts with HECTd3 and has similar subcellular localization.[4] The protein has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These function as the machinery for the homotypic fusion of late endosomes.[5]
Model organisms
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | Normal |
| Body weight | Normal |
| Anxiety | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Hot plate | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Body temperature | Normal |
| Eye morphology | Normal |
| Clinical chemistry | Normal |
| Haematology | Normal |
| Peripheral blood lymphocytes | Normal |
| Micronucleus test | Normal |
| Heart weight | Normal |
| Skin Histopathology | Normal |
| Brain histopathology | Normal |
| Eye Histopathology | Normal |
| Salmonella infection | Normal[6] |
| Citrobacter infection | Normal[7] |
| All tests and analysis from[8][9] |
Model organisms have been used in the study of STX8 function. A conditional knockout mouse line, called Stx8tm2a(EUCOMM)Wtsi[10][11] was generated as part of the International Knockout Mouse Consortium program—a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists—at the Wellcome Trust Sanger Institute.[12][13][14] Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[8][15] Twenty four tests were carried out on homozygous mutant adult mice, however no significant abnormalities were observed.[8]
Interactions
STX8 has been shown to interact with Vesicle-associated membrane protein 8,[16] VTI1B[17][18] and STX7.[16]
References
- ↑ "Three novel proteins of the syntaxin/SNAP-25 family". The Journal of Biological Chemistry 273 (51): 34171–9. Dec 1998. doi:10.1074/jbc.273.51.34171. PMID 9852078.
- ↑ "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)". Biochemical and Biophysical Research Communications 257 (2): 577–83. Apr 1999. doi:10.1006/bbrc.1999.0503. PMID 10198254.
- ↑ "Entrez Gene: STX8 syntaxin 8". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9482.
- ↑ "Interaction between syntaxin 8 and HECTd3, a HECT domain ligase". Cellular and Molecular Neurobiology 29 (1): 115–21. Feb 2009. doi:10.1007/s10571-008-9303-0. PMID 18821010.
- ↑ "Syntaxin 8 has two functionally distinct di-leucine-based motifs". Cellular & Molecular Biology Letters 13 (1): 144–54. October 2008. doi:10.2478/s11658-007-0043-9. PMID 17965969.
- ↑ "Salmonella infection data for Stx8". Wellcome Trust Sanger Institute. http://www.sanger.ac.uk/mouseportal/phenotyping/MAKV/salmonella-challenge/.
- ↑ "Citrobacter infection data for Stx8". Wellcome Trust Sanger Institute. http://www.sanger.ac.uk/mouseportal/phenotyping/MAKV/citrobacter-challenge/.
- ↑ 8.0 8.1 8.2 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
- ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ↑ "International Knockout Mouse Consortium". http://www.knockoutmouse.org/martsearch/search?query=Stx8.
- ↑ "Mouse Genome Informatics". http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4432376.
- ↑ "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. Jun 2011. doi:10.1038/nature10163. PMID 21677750.
- ↑ "Mouse library set to be knockout". Nature 474 (7351): 262–3. Jun 2011. doi:10.1038/474262a. PMID 21677718.
- ↑ "A mouse for all reasons". Cell 128 (1): 9–13. Jan 2007. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ "The mouse genetics toolkit: revealing function and mechanism". Genome Biology 12 (6): 224. 2011. doi:10.1186/gb-2011-12-6-224. PMID 21722353.
- ↑ 16.0 16.1 "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal 19 (23): 6453–64. Dec 2000. doi:10.1093/emboj/19.23.6453. PMID 11101518.
- ↑ "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. Oct 2005. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
- ↑ "Preferential association of syntaxin 8 with the early endosome". Journal of Cell Science 113 ( Pt 6) (6): 997–1008. Mar 2000. doi:10.1242/jcs.113.6.997. PMID 10683148.
Further reading
- "Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking". Molecular Biology of the Cell 10 (11): 3891–908. Nov 1999. doi:10.1091/mbc.10.11.3891. PMID 10564279.
- "Preferential association of syntaxin 8 with the early endosome". Journal of Cell Science 113 ( Pt 6) (6): 997–1008. Mar 2000. doi:10.1242/jcs.113.6.997. PMID 10683148.
- "Identification of CIP-1-associated regulator of cyclin B (CARB), a novel p21-binding protein acting in the G2 phase of the cell cycle". The Journal of Biological Chemistry 275 (30): 23181–6. Jul 2000. doi:10.1074/jbc.M001772200. PMID 10781590.
- "SNAREs contribute to the specificity of membrane fusion". Neuron 26 (2): 457–64. May 2000. doi:10.1016/S0896-6273(00)81177-0. PMID 10839363.
- "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal 19 (23): 6453–64. Dec 2000. doi:10.1093/emboj/19.23.6453. PMID 11101518.
- "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". The Journal of Biological Chemistry 276 (23): 19820–7. Jun 2001. doi:10.1074/jbc.M010838200. PMID 11278762.
- "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nature Structural Biology 9 (2): 107–11. Feb 2002. doi:10.1038/nsb746. PMID 11786915.
- "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum". The Biochemical Journal 368 (Pt 1): 29–39. Nov 2002. doi:10.1042/BJ20020845. PMID 12175335.
- "GS15 forms a SNARE complex with syntaxin 5, GS28, and Ykt6 and is implicated in traffic in the early cisternae of the Golgi apparatus". Molecular Biology of the Cell 13 (10): 3493–507. Oct 2002. doi:10.1091/mbc.E02-01-0004. PMID 12388752.
- "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochemical and Biophysical Research Communications 311 (3): 577–82. Nov 2003. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
- "Elevated expression of a regulator of the G2/M phase of the cell cycle, neuronal CIP-1-associated regulator of cyclin B, in Alzheimer's disease". Journal of Neuroscience Research 75 (5): 698–703. Mar 2004. doi:10.1002/jnr.20028. PMID 14991845.
- "Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity". Journal of Cell Science 117 (Pt 10): 1923–35. Apr 2004. doi:10.1242/jcs.01070. PMID 15039462.
- "High-throughput mapping of a dynamic signaling network in mammalian cells". Science 307 (5715): 1621–5. Mar 2005. doi:10.1126/science.1105776. PMID 15761153. Bibcode: 2005Sci...307.1621B.
- "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. Oct 2005. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
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