Chemistry:Alcian yellow

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Alcian yellow
Alcian yellow.svg
Names
IUPAC name
{(E)-1,2-Diazenediylbis[4,1-phenylene(6-methyl-1,3-benzothiazole-2,5-diyl)methylenesulfanediyl]}bis[(dimethylamino)-N,N-dimethylmethaniminium]
Other names
Ingrain Yellow 1
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
Properties
C40H46N8S4
Molar mass 767.11 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references
Tracking categories (test):

Alcian yellow is an azo dye with the formula (CH3[(CH3N)2CS]2C6H2SNCC6H4)2N2. Its structure consists of a largely conjugated array of benzene, benzothiazole and isothiouronium units.

Histological Use

Alcian yellow is used in histology in conjunction with toluidine blue to help visualize H. pylori.[1] The resulting structures are stained as follows:[2]

– Mucin: yellow

– H. pylori: blue

– Other cellular components/background: shades of blue

See also

References

  1. Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining Method for Helicobacter Pylori in Gastric Biopsies". Journal of Histotechnology 19 (2): 131–132. doi:10.1179/his.1996.19.2.131. ISSN 0147-8885. 
  2. "Helico*Stat Rapid Helicobacter Stain Kit Procedure". https://www.americanmastertech.com/instructions/kthst.pdf.