Attenuated total reflectance

Short description: Infrared spectroscopy sampling technique

Attenuated total reflection (ATR) is a sampling technique used in conjunction with infrared spectroscopy which enables samples to be examined directly in the solid or liquid state without further preparation.

Light undergoes multiple internal reflections in the crystal of high refractive index, shown in yellow. The sample is in contact with the crystal.

An ATR accessory for IR spectroscopy.

ATR uses a property of total internal reflection resulting in an evanescent wave. A beam of infrared light is passed through the ATR crystal in such a way that it reflects at least once off the internal surface in contact with the sample. This reflection forms the evanescent wave which extends into the sample. The penetration depth into the sample is typically between 0.5 and 2 micrometres, with the exact value determined by the wavelength of light, the angle of incidence and the indices of refraction for the ATR crystal and the medium being probed. The number of reflections may be varied by varying the angle of incidence. The beam is then collected by a detector as it exits the crystal. Most modern infrared spectrometers can be converted to characterise samples via ATR by mounting the ATR accessory in the spectrometer's sample compartment. The accessibility, rapid sample turnaround and ease of use of ATR with Fourier transform infrared spectroscopy (FTIR) has led to substantial use by the scientific community.

Typical materials for ATR crystals include germanium, KRS-5 and zinc selenide, while silicon is ideal for use in the Far-IR region of the electromagnetic spectrum. The excellent mechanical properties of diamond make it an ideal material for ATR, particularly when studying very hard solids, although the broad diamond phonon band between 2600 and 1900 cm⁻¹ significantly decreases signal to noise in this region. The shape of the crystal depends on the type of spectrometer and nature of the sample. With dispersive spectrometers, the crystal is a rectangular slab with chamfered edges, seen in cross-section in the illustrations. Other geometries use prisms, half-spheres, or thin sheets.

Applications

Recently, ATR-IR has been applied to microfluidic flows of aqueous solutions by engineering microreactors with built-in apertures for the ATR crystal, allowing the flow within microchannels to pass across the crystal surface for characterisation, or in dedicated flow cells. Due to the ATR geometry and the resulting evanescent wave, it is possible with this technique to study transport phenomena and sorption kinetics through thin films. The ability to passively characterise samples, with no sample preparation has also led to the use of ATR-FTIR in studying trace evidence in forensic science.

ATR-FTIR is also used as a tool in pharmacological research to investigate protein/pharmaceutical interactions in detail. Water-soluble proteins to be investigated require Polyhistidine-tags, allowing the macromolecule to be anchored to a lipid bilayer, which is attached to a Germanium crystal or other suitable optical media. Internal reflection with and without applied pharmaceutical or ligand will produce difference spectra to study conformational changes of the proteins upon binding.

See also

• Surface plasmon resonance

Bibliography

• Harrick, N.J. (1967). Internal Reflection Spectroscopy. John Wiley & Sons Inc. p. 342. ISBN 978-0-470-35250-2. 
• "Fourier Transform Infrared Spectroscopy (FT-IR)". Northwestern University Atomic and Nanoscale Characterization Experimental Center. http://www.nuance.northwestern.edu/keck-ii/keck-ii-instruments/ftir-spectroscopy/index.html.