Biography:Lucas Andrew Staehelin

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Short description: Swiss-American cell biologist


Lucas Andrew Staehelin
Born
Sydney, Australia
DiedSeptember 28, 2022(2022-09-28) (aged 83)
CitizenshipSwitzerland-America
Known forCell biology, photosynthesis, electron microscopy
TitleProfessor emeritus
Academic background
Alma materSwiss Federal Institute of Technology
Academic work
InstitutionsUniversity of Colorado Boulder

Lucas Andrew Staehelin (February 10, 1939 – September 28, 2022) was a retired Swiss-American cell biologist.[1] He was professor emeritus at the University of Colorado Boulder.[2]

He developed cryofixation methods and pioneered their use for preserving cellular structures for electron microscope studies.[3][4] Application of these methods to the analysis of plant, animal and bacterial cells brought insights into the nanoscale architecture and functional organization of membranous organelles and cytoskeletal systems.[5][6][7][8] Staehelin taught undergraduate and graduate courses in cellular and molecular biology at the University of Colorado Boulder.

Early life and education

Staehelin was born in Sydney, Australia on February 10, 1939.[citation needed] In 1948 he moved to Switzerland where he attended the Gymnasium in Bern. He studied natural sciences as an undergraduate at the Swiss Federal Institute of Technology, Zürich from 1959 to 1963, where he earned his doctorate in plant cell biology in 1966, studying in the laboratory of Kurt Mühlethaler.[9] From 1966 to 1969 he worked as a scientist at the Physics and Engineering Laboratory of the Department of Scientific and Industrial Research in New Zealand. He was a postdoctoral fellow from 1969 to 1970 at Harvard University.[citation needed][10]

Career

Staehelin became an assistant professor in the Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, in 1970, was promoted to associate professor in 1973, to full professor in 1978, and to professor emeritus in 2006. He held visiting professorships at Albert-Ludwigs University (1978), Swiss Federal Institute of Technology (1984, 1992), and University of Melbourne (1998).

He has authored and co-authored more than 300 scientific publications (H-Index 78).[11][12]

Research

Staehelin's research focused on using cryofixation methods to produce improved images of cellular structures by means of freeze-fracture, thin section and immunolabeling electron microscopy, and by electron tomography techniques.[4] His studies of photosynthetic membranes led to the characterization of differences in structure and function of grana and stroma thylakoid membranes,[13] of chlorosomes,[14] and the attachment of plastoglobules to thylakoids.[15] Vitrification of plant cells using propane-jet freezing led to the discovery that cellulose fibrils were synthesized by plasma membrane-associated rosette particle complexes,[16] and the demonstration that vesicle-mediated secretion and membrane recycling in turgid cells involves characteristic, transient membrane configurations.[17] High pressure freezing of intact plant tissues led to new models of plant cytokinesis, first as seen in thin section images[18][19][7] and then by electron tomography. These studies led to the discovery of the cell plate assembly matrix within which the assembly of the cell plate from vesicles occurs by previously unknown mechanisms.[20][21] Electron tomography analysis enabled his group to produce nanoscale models of endoplasmic reticulum, Golgi apparatus and trans Golgi network membranes[22][23][24][25][4] The Golgi matrix/scaffold was shown to originate on COPII vesicles and to capture passing Golgi to mediate ER-Golgi vesicle transfer as postulated by the stop-pluck-and-go hypothesis of Golgi trafficking.[26]

Awards

  • Recipient of the Alexander von Humboldt Research Award (1977)[27]
  • Member of the German National Academy of Sciences Leopoldina (1994)[28]
  • Fellow of the American Association for the Advancement of Science (2005)[29]
  • Recipient of the Haselkorn Scholar Award, University of Chicago (2006)[30]
  • First named Fellow of the American Society of Plant Physiologists (2007)[30]

References

  1. ↑ "L. Andrew Staehelin, CU Boulder molecular biologist, dies at 83" (in en). 2022-10-05. https://www.colorado.edu/asmagazine/2022/10/05/l-andrew-staehelin-cu-boulder-molecular-biologist-dies-83. 
  2. ↑ "Andrew Staehelin" (in en). 2016-09-22. https://www.colorado.edu/mcdb/andrew-staehelin. 
  3. ↑ Gilkey, John C.; Staehelin, L. Andrew (1986). "Advances in ultrarapid freezing for the preservation of cellular ultrastructure" (in en). Journal of Electron Microscopy Technique 3 (2): 177–210. doi:10.1002/jemt.1060030206. ISSN 1553-0817. https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.1060030206. 
  4. ↑ 4.0 4.1 4.2 Cheung, Alice Y; Cosgrove, Daniel J; Hara-Nishimura, Ikuko; Jürgens, Gerd; Lloyd, Clive; Robinson, David G; Staehelin, L Andrew; Weijers, Dolf (2021-09-15). "A rich and bountiful harvest: key discoveries in plant cell biology". The Plant Cell 34: 53–71. doi:10.1093/plcell/koab234. ISSN 1040-4651. PMID 34524464. 
  5. ↑ Nevo, Reinat; Charuvi, Dana; Tsabari, Onie; Reich, Ziv (April 2012). "Composition, architecture and dynamics of the photosynthetic apparatus in higher plants: The photosynthetic apparatus in higher plants" (in en). The Plant Journal 70 (1): 157–176. doi:10.1111/j.1365-313X.2011.04876.x. PMID 22449050. 
  6. ↑ Kirchhoff, Helmut (2019-03-08). "Chloroplast ultrastructure in plants". New Phytologist 223 (2): 565–574. doi:10.1111/nph.15730. ISSN 0028-646X. PMID 30721547. 
  7. ↑ 7.0 7.1 Jürgens, Gerd; Park, Misoon; Richter, Sandra; Touihri, Sonja; Krause, Cornelia; El Kasmi, Farid; Mayer, Ulrike (2015-01-26). "Plant cytokinesis: a tale of membrane traffic and fusion". Biochemical Society Transactions 43 (1): 73–78. doi:10.1042/BST20140246. ISSN 0300-5127. PMID 25619248. 
  8. ↑ Ogbu, Chinemerem P.; Roy, Sourav; Vecchio, Alex J. (January 2022). "Disruption of Claudin-Made Tight Junction Barriers by Clostridium perfringens Enterotoxin: Insights from Structural Biology" (in en). Cells 11 (5): 903. doi:10.3390/cells11050903. ISSN 2073-4409. PMID 35269525. 
  9. ↑ "Chemistry Tree - L. Andrew Staehelin". https://academictree.org/chemistry/peopleinfo.php?pid=438084. 
  10. ↑ "L. Andrew Staehelin". https://www.researchgate.net/profile/L-Staehelin. 
  11. ↑ "L. Andrew Staehelin, University of Colorado Boulder, Department of Molecular, Cellular, and Developmental Biology". https://www.researchgate.net/profile/L-Staehelin. 
  12. ↑ "Web of Science". https://www.webofscience.com/wos/woscc/citation-report/446b4e85-5162-4327-87b0-b30674531ad7-20d1cbb7. 
  13. ↑ Staehelin, L. Andrew (2003-04-01). "Chloroplast structure: from chlorophyll granules to supra-molecular architecture of thylakoid membranes" (in en). Photosynthesis Research 76 (1): 185–96. doi:10.1023/A:1024994525586. ISSN 1573-5079. PMID 16228577. 
  14. ↑ Staehelin, L. Andrew; Golecki, Jochen R.; Drews, Gerhart (1980-04-01). "Supramolecular organization of chlorosomes (chlorobium vesicles) and of their membrane attachment sites in Chlorobium Limicola" (in en). Biochimica et Biophysica Acta (BBA) - Bioenergetics 589 (1): 30–45. doi:10.1016/0005-2728(80)90130-9. ISSN 0005-2728. PMID 7356977. 
  15. ↑ Rottet, Sarah; Besagni, Céline; Kessler, Felix (2015-09-01). "The role of plastoglobules in thylakoid lipid remodeling during plant development" (in en). Biochimica et Biophysica Acta (BBA) - Bioenergetics. SI: Chloroplast Biogenesis 1847 (9): 889–99. doi:10.1016/j.bbabio.2015.02.002. ISSN 0005-2728. PMID 25667966. 
  16. ↑ Giddings, T H Jr; Brower, D L; Staehelin, L A (1980-02-01). "Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls.". Journal of Cell Biology 84 (2): 327–339. doi:10.1083/jcb.84.2.327. ISSN 0021-9525. PMID 7189756. 
  17. ↑ Staehelin, L. A.; Chapman, R. L. (1987-05-01). "Secretion and membrane recycling in plant cells: novel intermediary structures visualized in ultrarapidly frozen sycamore and carrot suspension-culture cells" (in en). Planta 171 (1): 43–57. doi:10.1007/BF00395066. ISSN 1432-2048. PMID 24227269. 
  18. ↑ Samuels, A L; Giddings, T H Jr; Staehelin, L A (1995-09-15). "Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants.". Journal of Cell Biology 130 (6): 1345–1357. doi:10.1083/jcb.130.6.1345. ISSN 0021-9525. PMID 7559757. 
  19. ↑ Otegui, Marisa; Staehelin, L. Andrew (2000-06-01). "Syncytial-Type Cell Plates: A Novel Kind of Cell Plate Involved in Endosperm Cellularization of Arabidopsis". The Plant Cell 12 (6): 933–947. doi:10.1105/tpc.12.6.933. ISSN 1040-4651. PMID 10852938. 
  20. ↑ Otegui, M. S.; Mastronarde, D. N.; Kang, B. H.; Bednarek, S. Y.; Staehelin, L. A. (September 2001). "Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualized by high resolution electron tomography". The Plant Cell 13 (9): 2033–2051. doi:10.1105/tpc.13.9.2033. ISSN 1040-4651. PMID 11549762. 
  21. ↑ Otegui, Marisa S. (2011), Popper, Zoë A., ed., "Electron Tomography and Immunogold Labelling as Tools to Analyse de Novo Assembly of Plant Cell Walls" (in en), The Plant Cell Wall: Methods and Protocols, Methods in Molecular Biology (Totowa, NJ: Humana Press) 715: pp. 123–140, doi:10.1007/978-1-61779-008-9_9, ISBN 978-1-61779-008-9, PMID 21222081 
  22. ↑ ROBINSON, DAVID G. (2020-05-27). "Plant Golgi ultrastructure". Journal of Microscopy 280 (2): 111–121. doi:10.1111/jmi.12899. ISSN 0022-2720. PMID 32420623. 
  23. ↑ Staehelin, L. Andrew; Kang, Byung-Ho (2008-08-01). "Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography". Plant Physiology 147 (4): 1454–1468. doi:10.1104/pp.108.120618. ISSN 0032-0889. PMID 18678738. 
  24. ↑ Kang, Byung-Ho; Nielsen, Erik; Preuss, Mary Lai; Mastronarde, David; Staehelin, L. Andrew (2011). "Electron Tomography of RabA4b- and PI-4Kβ1-Labeled Trans Golgi Network Compartments in Arabidopsis" (in en). Traffic 12 (3): 313–329. doi:10.1111/j.1600-0854.2010.01146.x. ISSN 1600-0854. PMID 21134079. 
  25. ↑ Donohoe, Bryon S.; Kang, Byung-Ho; Gerl, Mathias J.; Gergely, Zachary R.; McMichael, Colleen M.; Bednarek, Sebastian Y.; Staehelin, L. Andrew (2013). "Cis-Golgi Cisternal Assembly and Biosynthetic Activation Occur Sequentially in Plants and Algae" (in en). Traffic 14 (5): 551–567. doi:10.1111/tra.12052. ISSN 1600-0854. PMID 23369235. 
  26. ↑ Nebenführ, Andreas; Gallagher, Larry A.; Dunahay, Terri G.; Frohlick, Jennifer A.; Mazurkiewicz, Anna M.; Meehl, Janet B.; Staehelin, L. Andrew (1999-12-01). "Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System1". Plant Physiology 121 (4): 1127–1141. doi:10.1104/pp.121.4.1127. ISSN 0032-0889. PMID 10594100. 
  27. ↑ "Singleview - Alexander von Humboldt-Stiftung". https://www.humboldt-foundation.de/en/connect/explore-the-humboldt-network/singleview?tx_rsmavhsolr_solrview%5BpPersonId%5D=1027209&cHash=f5f616ad6e075c4cf09b39f2fe3365f0. 
  28. ↑ "List of Members". https://www.leopoldina.org/en/members/list-of-members/list-of-members/member/Member/show/lucas-andrew-staehelin/. 
  29. ↑ "Historic Fellows" (in en). American Association for the Advancement of Science. https://www.aaas.org/fellows/historic. 
  30. ↑ 30.0 30.1 "CU-Boulder's Andrew Staehelin Named First Ever Fellow Of American Society Of Plant Biologists" (in en). 2007-07-25. https://www.colorado.edu/today/2007/07/25/cu-boulders-andrew-staehelin-named-first-ever-fellow-american-society-plant-biologists. 



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