Biology:CHKB (gene)

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

Choline kinase beta (CK), also known as Ethanolamine kinase (EK), Choline kinase-like protein , choline/ethanolamine kinase beta (CKEKB), or Choline/ethanolamine kinase is a protein encoded by the CHKB gene. This gene is found on chromosome 22 in humans. The encoded protein plays a key role in phospholipid biosynthesis. Choline kinase (CK) and ethanolamine kinase (EK) catalyzes the first step in phosphatidylethanolamine biosynthesis. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus.

Structure

The CHKB gene is located on the q arm of chromosome 22 at position 13.3 and it spans 4,041 base pairs. The CHKB gene produces a 7 kDa protein composed of 60 amino acids. The structure of the protein has been found to be a homodimer, and forms a heterodimer with the CHKA protein. It has also been found to contain multiple highly conserved domains, such as a Brenner phosphotransferase consensus sequence essential in catalytic function.

Function

The CHKB gene encodes for a key protein in phospholipid biosynthesis. The choline kinase (CK) and ethanolamine kinase (EK) proteins, which are coded by the CHKB gene, catalyze the phosphorylation of choline/ethanolamine in vitro to phosphocholine/phosphoethanolamine. The catalysis is controlled by ATP in the presence of magnesium and ADP, and commits choline to the enzymatic pathway for biosynthesis of phosphatidylcholine. This is the first step in the biosynthesis of phosphocholine/phosphoethanolamine in all animal cells, and is done by the Kennedy pathway. The highly purified choline kinases from mammalian sources and their recombinant gene products have been shown to have ethanolamine kinase activity as well, indicating that both activities reside on the same protein. However, it has been shown that the protein has higher activity with ethanolamine and may not significantly contribute to in vivo phosphatidylcholine biosynthesis. The choline kinase-like protein encoded by CHKB belongs to the choline/ethanolamine kinase family; however, its exact function is not known. At least two transcript variants encoding two different isoforms have been found for this gene, and one of the transcripts is bicistronic.

Clinical significance

Mutations in CHKB have been found to result in mitochondrial deficiencies and associated disorders. Knockdown of the gene has been known to result in decreased choline kinase and phosphatidylcholine activity. This impairment in activity may lead to a modified composition of the phospholipid composition in the mitochondrial membrane resulting in major disorders in the function and structure of the mitochondria. Major disorders include as Megaconial Congenital Muscular Dystrophy (MDCMC), and Narcolepsy.

Megaconial Congenital Muscular Dystrophy (MDCMC)

CHKB mutations have been majorly associated with Megaconial Congenital Muscular Dystrophy (MDCMC). Megaconial Congenital Muscular Dystrophy (MDCMC) is an autosomal recessive congenital muscular dystrophy characterized by muscle biopsy results displaying an enlarged mitochondria which are common in the periphery of the fibers but scarce around the center.

Common clinical manifestations of MDCMC include:

• early-onset hypotonia
• muscle wasting
• mildly elevated serum creatine kinase (CK) levels
• severe intellectual disability without brain structural abnormalities
• Gower's sign
• mental retardation
• fatal cardiomyopathy

Symptoms such as neurogenic atrophy, enlarged mitochondria in the periphery of the fibers, and complex I deficiency were shown in a Spanish patient with a homozygous mutation of c.810T>A. Another patient with a homozygous mutation (p.E292X) in the CHKB gene exhibited rhythmic jerkings of arms, which were characterized as muscle spasms. Finally, a patient with a homozygous c.810T>A showed signs of Gower's sign, hypotonia, and proximal muscle weakness.

Narcolepsy

Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.

Interactions

CHKB has been shown to have Protein-protein interactions with the following.

• ALB
• Human serum albumin

External links

• Human CHKB genome location and CHKB gene details page in the UCSC Genome Browser.
• Human CKB genome location and CKB gene details page in the UCSC Genome Browser.

Further reading

• "Structure and function of choline kinase isoforms in mammalian cells". Prog. Lipid Res. 43 (3): 266–81. 2004. doi:10.1016/j.plipres.2003.12.001. PMID 15003397. 
• "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. 2004. doi:10.1101/gr.2596504. PMID 15489334. 
• "A genome annotation-driven approach to cloning the human ORFeome". Genome Biol. 5 (10): R84. 2005. doi:10.1186/gb-2004-5-10-r84. PMID 15461802. 
• "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. 2004. doi:10.1038/ng1285. PMID 14702039. 
• "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. 2003. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M. 
• "Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping". DNA Res. 8 (1): 1–9. 2001. doi:10.1093/dnares/8.1.1. PMID 11258795. 
• "Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I". J. Biol. Chem. 275 (41): 31739–46. 2000. doi:10.1074/jbc.M006322200. PMID 10918069. 
• "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics 60 (3): 295–308. 1999. doi:10.1006/geno.1999.5927. PMID 10493829. 
• "Complementary DNA sequence for a 42 kDa rat kidney choline/ethanolamine kinase". Biochim. Biophys. Acta 1390 (1): 1–7. 1998. doi:10.1016/s0005-2760(97)00177-x. PMID 9487136. 
• "Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I". FEBS Lett. 409 (3): 401–6. 1997. doi:10.1016/S0014-5793(97)00561-9. PMID 9224698. 

This article incorporates text from the United States National Library of Medicine, which is in the public domain.