Biology:Cajal body
Cajal bodies (CBs) also coiled bodies, are spherical nuclear bodies of 0.3–1.0 µm in diameter found in the nucleus of proliferative cells like embryonic cells and tumor cells, or metabolically active cells like neurons. CBs are membrane-less organelles and largely consist of proteins and RNA. They were first reported by Santiago Ramón y Cajal in 1903, who called them nucleolar accessory bodies due to their association with the nucleoli in neuronal cells.[1] They were rediscovered with the use of the electron microscope (EM) and named coiled bodies, according to their appearance as coiled threads on EM images, and later renamed after their discoverer.[2] Research on CBs was accelerated after discovery and cloning of the marker protein p80/Coilin.[3] CBs have been implicated in RNA-related metabolic processes such as the biogenesis, maturation and recycling of snRNPs, histone mRNA processing and telomere maintenance. CBs assemble RNA which is used by telomerase to add nucleotides to the ends of telomeres.[4]
History
CBs were initially discovered by neurobiologist Santiago Ramón y Cajal in 1903 as small argyrophilic (readily stained by silver salts, literally "silver loving") spots in the nuclei of silver-stained neuronal cells. Because of their close association with nucleoli, he named them nucleolar accessory bodies. Later on, they were forgotten and rediscovered multiple times independently which led to a state where scientists from different research fields used different names for the same structure. Names used for CBs included "sphere organelles", "Binnenkörper", "nucleolar bodies" or "coiled bodies". The name coiled bodies comes from observation of electron microscopists Monneron and Bernhard. They described bodies as aggregates composed of coiled threads with thickness of 400–600 Å. When using higher magnification, they appear as tiny, 50 Å thick fibrils irregularly twisted along the axis of the threads. The bodies were even predicted to consist of ribonucleoproteins since treatment of cells with protease and RNase together, but not alone, caused dramatic changes to the structure of CBs.[5]
Localization
Cajal bodies are only found in nuclei of plant, yeast, and animal cells.[6] The cells in which Cajal bodies are most apparent usually demonstrate high levels of transcriptional activity, and are often dividing rapidly.[7]
Cell cycle
They are about 0.1–2.0 micrometres and are found one to five per nucleus. The number varies in different types of cells and over the cell cycle. Maximum number is reached in mid G1 phase and towards G2 they become larger and their number decreases. CBs disassemble during the M phase and reappear again later in G1 phase. Cajal bodies are possibly sites of assembly or modification of the transcription machinery of the nucleus.[8]
Functions
CBs are bound to the nucleolus by coilin proteins. P80-coilin is a specific marker for coiled bodies,[9] and demonstrates these bodies tend to be associated with the nucleolus when cells are not dividing. CBs are associated with telomerase assembly and recruitment via a CAB-RNA sequence common in both CB RNAs (scaRNAs) and the RNA component of telomerase (TERC). TCAB1 recognizes the CAB sequence in both and recruits telomerase to the CBs.
CBs contain high concentrations of splicing small nuclear ribonucleoproteins (snRNPs), possibly indicating that they function to modify RNA after it has been transcribed from DNA.[10] Experimental evidence indicates that CBs contribute to the biogenesis of enzyme telomerase, and assist in subsequent transport of telomerase to telomeres.[11][12]
References
- ↑ Cajal SR (1903). "Un sencillo metodo de coloracion selectiva del reticulo protoplasmico y sus efectos en los diversos organos nerviosos de vertebrados e invertebrados". Trab Lab Investig Biol Univ Madr 2: 129–221.
- ↑ "Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes". Molecular Biology of the Cell 10 (12): 4385–4402. December 1999. doi:10.1091/mbc.10.12.4385. PMID 10588665.
- ↑ "Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin". The Journal of Experimental Medicine 173 (6): 1407–1419. June 1991. doi:10.1084/jem.173.6.1407. PMID 2033369.
- ↑ "Processive and distributive extension of human telomeres by telomerase under homeostatic and nonequilibrium conditions". Molecular Cell 42 (3): 297–307. May 2011. doi:10.1016/j.molcel.2011.03.020. PMID 21549308.
- ↑ "Fine structural organization of the interphase nucleus in some mammalian cells". Journal of Ultrastructure Research 27 (3): 266–288. May 1969. doi:10.1016/S0022-5320(69)80017-1. PMID 5813971.
- ↑ "Cajal bodies: a long history of discovery". Annual Review of Cell and Developmental Biology 21 (1): 105–131. 2005. doi:10.1146/annurev.cellbio.20.010403.103738. PMID 16212489.
- ↑ "Cajal bodies and coilin--moving towards function". The Journal of Cell Biology 159 (1): 17–21. October 2002. doi:10.1083/jcb.200206111. PMID 12379800.
- ↑ "Chromosome territories, nuclear architecture and gene regulation in mammalian cells". Nature Reviews. Genetics 2 (4): 292–301. April 2001. doi:10.1038/35066075. PMID 11283701.
- ↑ "Association between the nucleolus and the coiled body". Journal of Structural Biology 104 (1–3): 120–127. 1990. doi:10.1016/1047-8477(90)90066-L. PMID 2088441.
- ↑ "The Cajal body and histone locus body". Cold Spring Harbor Perspectives in Biology 2 (7): a000653. July 2010. doi:10.1101/cshperspect.a000653. PMID 20504965.
- ↑ "Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres". Molecular Biology of the Cell 17 (2): 944–954. February 2006. doi:10.1091/mbc.E05-09-0904. PMID 16319170.
- ↑ "Cell cycle-regulated trafficking of human telomerase to telomeres". Molecular Biology of the Cell 17 (2): 955–965. February 2006. doi:10.1091/mbc.E05-09-0903. PMID 16339074.
 |  |
