Biology:DIPK1B
DIPK1B or divergent protein kinase domain 1B is a protein that in humans is coded by the DIPK1B gene.[1] It is a member of the FAM69 family, which consists of three members across all vertebrates other than fish. Is is also known as FAM69B.[2]
Gene
The DIPK1B gene is found on human chromosome 9, in the 9q34.3 band.[2] The gene is located on the plus strand and spans 12,275 bases.[3] The gene contains five exons. It is a member of FAM69, a family of genes with three members, DIPK1A, DIPK1B, and DIPK1C.[4] This family is present in all vertebrates, with slight differences in fish, and orthologs of highly conserved structure are found in all metazoa.[4]
Transcripts
In humans there is only one known DIPK1B transcript. The mRNA transcript of DIPK1B is 2358 bases long, and contains all five exons of the DIPK1B gene.[5]
Protein
Human DIPK1B has only one isoform. The resultant protein is 431 amino acids long, has an isoelectric point of 9, and has a predicted molecular weight of 48.6 kDa.[1][8] DIPK1B contains two distinct domains, a transmembrane domain and a protein kinase domain.[1]
Expression
DIPK1B exhibits variable ubiquitous expression at moderate to high levels.[9] Expression in the brain, heart, and lung is notably higher than in other tissues. [2]
Regulation
DIPK1B is localized to the membrane of the endoplasmic reticulum.[4] It is expected to undergo a number of post-translational modifications, including o-linked glycosylation, phosphorylation, ubiquitination, and acetylation.[10]
Homology
| Genus and Species | Common Name | Taxonomic Group | Date of Divergence (MYA) | Accession Number | Sequence Length (aa) | Sequence Identity to Human Protein (%) | Sequence Similarity to Human Protein (%) |
|---|---|---|---|---|---|---|---|
| Homo sapiens | Humans | Primates | 0 | NP_689634 | 431 | 100 | 100 |
| Gorilla gorilla gorilla | Gorilla | Primates | 8.6 | XP_004048941.2 | 431 | 99.5 | 99.5 |
| Desmodus rotundus | Vampire bat | Mammals | 94 | XP_053775135 | 435 | 84.9 | 90.7 |
| Dromiciops gliroides | Monito del monte | Marsupials | 160 | XP_043845610.1 | 431 | 71.2 | 83.5 |
| Gopherus flavomarginatus | Bolson tortoise | Reptiles | 319 | XP_050782714.1 | 431 | 68.2 | 82.6 |
| Tiliqua scincoides | Common blue-tongued skink | Reptiles | 319 | XP_066466737.1 | 431 | 66.8 | 81.4 |
| Varanus komodoensis | Komodo dragon | Reptiles | 319 | XP_044309118.1 | 431 | 65.7 | 90 |
| Cyrtonyx montezumae | Montezuma quail | Birds | 319 | XP_065593300.1 | 431 | 67.5 | 82.6 |
| Gallus gallus | Chicken | Birds | 319 | XP_415423 | 431 | 67.3 | 82.4 |
| Taeniopygia guttata | Australian zebra finch | Birds | 319 | XP_002199526.4 | 431 | 66.1 | 81.7 |
| Geothlypis trichas | Common yellowthroat | Birds | 319 | KAL9827061.1 | 431 | 65.9 | 82.4 |
| Ambystoma mexicanum | Axolotl | Amphibians | 352 | XP_069465698.1 | 431 | 67.5 | 81 |
| Ascaphus truei | Tailed Frog | Amphibians | 352 | XP_075435024 | 431 | 66.1 | 82.6 |
| Xenopus laevis | African clawed frog | Amphibians | 352 | XP_018088073.1 | 431 | 62.9 | 80.3 |
| Protopterus annectens | West African Lungfish | Fish | 408 | XP_043914470 | 431 | 65.4 | 82.4 |
| Hypanus sabinus | Atlantic stingray | Fish | 462 | XP_059850265.1 | 431 | 61.5 | 80.3 |
| Callorhinchus milii | Australian ghostshark | Fish | 462 | XP_042193771.1 | 431 | 60.8 | 78.4 |
| Amblyraja radiata | Thorny skate | Fish | 462 | XP_032904599 | 431 | 58.9 | 79.1 |
| Magallana gigas | Pacific Oyster | Mollusks | 686 | XP_011453093.2 | 438 | 23.7 | 40 |
| Anopheles gambiae | Mosquito | Insects | 686 | XP_061498526 | 427 | 23 | 37.3 |
Function
DIPK1B has been shown to interact with SAT1 and SLC39A12.[11]
References
- ↑ 1.0 1.1 1.2 "NCBI Protein - divergent protein kinase domain 1B [Homo sapiens"]. https://www.ncbi.nlm.nih.gov/protein/NP_689634.2.
- ↑ 2.0 2.1 2.2 "NCBI Gene - DIPK1B divergent protein kinase domain 1B [Homo sapiens (human)"]. https://www.ncbi.nlm.nih.gov/gene/138311.
- ↑ "GeneCards- DIPK1B Gene". https://www.genecards.org/cgi-bin/carddisp.pl?gene=DIPK1B.
- ↑ 4.0 4.1 4.2 "Characterisation of the FAM69 family of cysteine-rich endoplasmic reticulum proteins". Biochemical and Biophysical Research Communications 406 (3): 471–477. March 2011. doi:10.1016/j.bbrc.2011.02.076. PMID 21334309. Bibcode: 2011BBRC..406..471T.
- ↑ "NCBI Nuccore - Homo sapiens divergent protein kinase domain 1B (DIPK1B), mRNA". 27 April 2025. https://www.ncbi.nlm.nih.gov/nuccore/NM_152421.4.
- ↑ "AlphaFold Protein Structure Database". https://alphafold.ebi.ac.uk/entry/AF-Q5VUD6-F1.
- ↑ "iCn3D: Web-based 3D Structure Viewer". https://www.ncbi.nlm.nih.gov/Structure/icn3d/full.html.
- ↑ "Expasy - Compute pI/Mw tool". https://web.expasy.org/compute_pi/.
- ↑ "GDS3113 / 137763". https://www.ncbi.nlm.nih.gov/geo/tools/profileGraph.cgi?ID=GDS3113:137763.
- ↑ "DIPK1B (human)". https://www.phosphosite.org/proteinAction.action?id=12672465&showAllSites=true.
- ↑ "IntAct Portal". https://www.ebi.ac.uk/intact/search?query=DIPK1B#interactions.
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