Biology:Isopenicillin N epimerase

From HandWiki
isopenicillin N epimerase
Identifiers
EC number5.1.1.17
CAS number88201-43-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, an isopenicillin N epimerase (EC 5.1.1.17) is an enzyme that catalyzes the chemical reaction

isopenicillin N [math]\displaystyle{ \rightleftharpoons }[/math] penicillin N

Hence, this enzyme has one substrate, isopenicillin N, and one product, penicillin N.

This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on amino acids and derivatives. The systematic name of this enzyme class is penicillin N 5-amino-5-carboxypentanoyl-epimerase. This enzyme participates in penicillin and cephalosporin biosynthesis.

References

  • "Purification and properties of isopenicillin N epimerase from Streptomyces clavuligerus". Biochim. Biophys. Acta 999 (1): 78–85. 1989. doi:10.1016/0167-4838(89)90033-2. PMID 2804141. 
  • "Purification and characterization of the isopenicillin-N epimerase from Nocardia lactamdurans". J. Gen. Microbiol. 136 (4): 663–671. 1990. doi:10.1099/00221287-136-4-663. 
  • "Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway". Proceedings of the Royal Society B 248 (1323): 283–9. 1992. doi:10.1098/rspb.1992.0073. PMID 1354366. 
  • "Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C Function, evolution, refolding, and enzyme engineering". Ann. N.Y. Acad. Sci. 672 (1 Enzyme Engine): 396–408. 1992. doi:10.1111/j.1749-6632.1992.tb32705.x.