Biology:Nicking enzyme
A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved.[1][2] They can be used for strand-displacement amplification,[3] Nicking Enzyme Amplification Reaction, exonucleotyic degradation, the creation of small gaps,[4] or nick translation.[5] The latter process has been successfully used to incorporate both radioactively labelled nucleotides and fluorescent nucleotides allowing specific regions on a double stranded DNA to be studied.[5][6] Over 200 nicking enzymes have been studied, and 13 of these are available commercially[7] and are routinely used for research and in commercial products.
References
- ↑ Ando T (July 1969). "Isolation and characterization of enzymes with nicking action from phage T4-infected Escherichia coli". J. Biochem. 66 (1): 1–10. doi:10.1093/oxfordjournals.jbchem.a129107. PMID 4309718.
- ↑ "Characterization of the specific DNA nicking activity of restriction endonuclease N.BstNBI". Biol. Chem. 381 (11): 1123–5. November 2000. doi:10.1515/BC.2000.137. PMID 11154070.
- ↑ "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system". Proc. Natl. Acad. Sci. U.S.A. 89 (1): 392–6. Jan 1992. doi:10.1073/pnas.89.1.392. PMID 1309614. Bibcode: 1992PNAS...89..392W.
- ↑ "Simple and rapid preparation of gapped plasmid DNA for incorporation of oligomers containing specific DNA lesions". Mol. Biotechnol. 19 (2): 133–40. October 2001. doi:10.1385/MB:19:2:133. PMID 11725483.
- ↑ 5.0 5.1 "Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I". J. Mol. Biol. 113 (1): 237–51. June 1977. doi:10.1016/0022-2836(77)90052-3. PMID 881736.
- ↑ "A single-molecule barcoding system using nanoslits for DNA analysis". Proc. Natl. Acad. Sci. U.S.A. 104 (8): 2673–8. 2007. doi:10.1073/pnas.0611151104. PMID 17296933. Bibcode: 2007PNAS..104.2673J.
- ↑ "REBASE Enzymes". Encyclopedia of restriction and nicking enzymes. http://rebase.neb.com/cgi-bin/azlist?nick. Retrieved 2009-06-01.
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