Biology:PfSPZ Vaccine

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Short description: Malaria vaccine

PfSPZ Vaccine is a metabolically active non-replicating whole sporozoite (SPZ) malaria vaccine being developed by Sanaria against Plasmodium falciparum (Pf) malaria. Clinical trials have been safe, extremely well tolerated and highly efficacious. The first generation PfSPZ product is attenuated by gamma irradiation; the second generation vaccines PfSPZ-CVac and PfSPZ LARC2 are, respectively, attenuated chemically and genetically. Multiple studies are ongoing with trials of the PfSPZ vaccines. All three products are produced using the same manufacturing process. These products are stored and distributed below -150 °C using liquid nitrogen (LN2) vapor phase (LNVP) freezers and cryoshippers.

History

In the first half of the 20th century there were first attempts to protect people from malaria.[citation needed] At the beginning Pasteur's approach of developing bacterial vaccines was used as a big hope in eradication of this fatal disease. But inactivated malaria sporozoites (by formalin) were ineffective in inducing the protection.[citation needed]

In 1948 inactivated merozoites with an adjuvant were used for preventing lethal malaria to kill a group of monkeys. But the strong toxicity of the adjuvant and inability to obtain sufficient count of parasites from human blood stopped further efforts in this way.[1]

In 1967 irradiated malaria sporozoites (extracted from salivary glands of infected mosquitos) induced immune response in mice without the need of the adjuvant and similar evidence obtained in human volunteer trials. The mice were exposed to irradiated mosquitos infected by malaria parasites. Mice and volunteers did not acquire malaria because mosquitos and the sporozoites were irradiated and their immune cells triggered response that could protect them from following infection.[2][3] Yet this approach was not further developed due to problems with obtaining sufficient number of sporozoites and with the harvesting of parasites.[citation needed]

Later, modern adjuvants and the possibility of preparing of single parasite proteins provided another way to create a malaria vaccine. RTS,S is a subunit vaccine based on coat protein of sporozoites of the Plasmodium falciparum. The RTS,S vaccine was endorsed by the World Health Organization in October 2021 for broad use in children, making it the first malaria vaccine to receive this recommendation.[4] (As of April 2022), 1 million children in Ghana, Kenya and Malawi have received at least one shot of the vaccine, with more doses being provided as the vaccine production ramps up.[5] RTS,S reduces hospital admissions from severe malaria by around 30%.[5]

PfSPZ development

In 2003 Sanaria ran trials in which falciparum sporozoites were manually dissected from salivary glands of mosquitos, irradiated and preserved before inoculation with one goal: to develop and commercialize a non-replicating, metabolically active PfSPZ vaccine.[6]

In human volunteer trials PfSPZ was applied subcutaneously (SC) or intradermally (ID) and such as it showed only modest immune response. When PfSPZ Vaccine was injected intravenously (IV) to nonhuman primates or mice it finally triggers CD8+ T-cells producing IFNγ. These T cells are believed to be the main immunologic mechanism to fight malaria in liver.

Two first clinical trials of IV administration of PfSPZ were conducted in 2013. Previous ID or IC clinical trials didn't trigger adequate immune response. A 2014 phase 1 trial with the PfSPZ Vaccine found that more than half of the participants were protected from malaria infection for over a year after the trial.[7][8]

In 2014 Sanaria promoted an Indiegogo campaign to develop a robot that could dissect salivary glands of mosquitos, to make preparation and further development of vaccine much faster and easier.[9] The crowdfunding campaign ended, after being backed by $45,024 of the $250,000 goal.[10]

The PfSPZ Vaccine candidate was granted fast track designation by the U.S. Food and Drug Administration in September 2016.[11]

A study published in 2017 reported complete protection after 10 weeks with three doses of PfSPZ-CVac.[12] In April 2019, a phase 3 trial in Bioko was announced, scheduled to start in early 2020.[13] Another study of the PfSPZ vaccine was published in December 2022, reporting vaccine efficacy at up to 48% at 6 months follow up, and up to 46% efficacy at 18 months.[14][15]

Mechanism

CD8+ T cells play a key role in killing Plasmodium developing in the liver. Mice or monkeys which received monoclonal antibody to the CD8 lost protection by this type of vaccine. Once the antibody application was stopped, the protection was returned.[16][17] Plasmodium is injected by infected mosquito into the bloodstream of the host in the form of sporozoites, which travel to the liver and invade liver cells, where sporozoites divide and produce tens of thousands merozoites per one cell. RTS,S is prepared to stop malaria in the phase after the injection. The PfSPZ vaccine is made of attenuated sporozites, which are active and travel to liver cells, where CD8+ T cells producing IFNγ are activated. Frequencies of PfSPZ-specific CD3+CD4+, CD3+CD8+, CD3+γδ T cells are dose-dependent. PfSPZ-specific CD3+CD8+ T cells were found in 7 of 12 protected subjects in a human volunteer trial.[18] These cells are required for protection in most individuals and are primarily situated in the liver because of the persistence of parasite antigens and retained as tissue memory cells.[19]

Distribution

PfSPZ vaccines are cryopreserved and stored in LNVP freezers[20] below -150 °C and distributed using dry vapor cryoshippers that also maintain temperature below -150 °C. Cryoshippers[21] are self-contained mobile storage units that have hold times of ~14 to 28 days or more depending on model and packaging and are highly suited for last-mile transportation, particularly in Africa. Cryoshippers are used extensively in the livestock breeding, CAR-T and cellular therapies industries. LNVP distribution uses a simple hub-and-spoke model [22] and cryoshippers stay at the immunization sites as temporary storage units that may be recharged with LN2. Advantages of the LNVP cold chain are a) independence from electricity, b) no requirement for fridges, freezers or refrigerated transport, c) no narrow temperature requirements, d) reduced chances for temperature deviations, e) no moving parts, and f) energy efficiency. LN2 is widely available, including in African countries, making LNVP distribution easier than the 2-8 °C and the dry ice and ultralow freezer-based cold chains of Ervebo (vs ebola)[23][24] and certain SARS-CoV-2[25] vaccines. Modeling LNVP distribution[26] also indicated costs would be no different per 3-dose regimen than the 2-8 °C cold chain for lyophilized vaccines.

References

  1. ↑ Freund, J; Thomson, K. J. (1948). "Immunization of monkeys against malaria by means of killed parasites with adjuvants". The American Journal of Tropical Medicine and Hygiene 28 (1): 1–22. doi:10.4269/ajtmh.1948.s1-28.1. PMID 18898694. 
  2. ↑ Nussenzweig, R. S.; Vanderberg, J; Most, H; Orton, C (1967). "Protective immunity produced by the injection of x-irradiated sporozoites of plasmodium berghei". Nature 216 (5111): 160–2. doi:10.1038/216160a0. PMID 6057225. Bibcode: 1967Natur.216..160N. 
  3. ↑ Rieckmann, K. H.; Carson, P. E.; Beaudoin, R. L.; Cassells, J. S.; Sell, K. W. (1974). "Letter: Sporozoite induced immunity in man against an Ethiopian strain of Plasmodium falciparum". Transactions of the Royal Society of Tropical Medicine and Hygiene 68 (3): 258–9. doi:10.1016/0035-9203(74)90129-1. PMID 4608063. 
  4. ↑ "A 'Historical Event': First Malaria Vaccine Approved by W.H.O.". The New York Times. 6 October 2021. https://www.nytimes.com/2021/10/06/health/malaria-vaccine-who.html. 
  5. ↑ 5.0 5.1 "First malaria vaccine hits 1 million dose milestone — although it has its shortcomings". 13 May 2022. https://www.npr.org/sections/goatsandsoda/2022/05/13/1098536246/first-malaria-vaccine-hits-1-million-dose-milestone-although-it-has-its-shortcom. 
  6. ↑ Luke, T. C.; Hoffman, S. L. (2003). "Rationale and plans for developing a non-replicating, metabolically active, radiation-attenuated Plasmodium falciparum sporozoite vaccine". The Journal of Experimental Biology 206 (Pt 21): 3803–8. doi:10.1242/jeb.00644. PMID 14506215. 
  7. ↑ "Protection against malaria at 1 year and immune correlates following PfSPZ vaccination". Nature Medicine 22 (6): 614–623. 2016. doi:10.1038/nm.4110. PMID 27158907. https://zenodo.org/record/1233457. 
  8. ↑ Feller, Stephen (2016-05-10). "Malaria vaccine shown to be safe, effective in phase 1 trial - More than half of volunteers in the small study did not contract malaria when exposed to mosquitoes more than a year after their last dose of the vaccine.". https://www.upi.com/Health_News/2016/05/10/Malaria-vaccine-shown-to-be-safe-effective-in-phase-1-trial/8691462892136/. 
  9. ↑ "Sanaria Inc. To Launch Crowdfunding Campaign For Sporobottm, A Mosquito-Dissecting Robot For Accelerating Manufacture Of Sanaria's Malaria Vaccine". Sanaria. 2014-04-30. https://sanaria.com/2014/04/30/sanaria-inc-to-launch-crowdfunding-campaign-for-sporobottm-a-mosquito-dissecting-robot-for-accelerating-manufacture-of-sanarias-malaria-vaccine/. 
  10. ↑ "Malaria Vaccine Robot - Robot vs. Mosquito Sanaria - SporoBot". https://www.indiegogo.com/projects/malaria-vaccine-robot-robot-vs-mosquito-sanaria-sporobot#/. 
  11. ↑ "SANARIA PfSPZ VACCINE AGAINST MALARIA RECEIVES FDA FAST TRACK DESIGNATION". Sanaria Inc.. 2016-09-22. http://www.sanaria.com/pdf/Fast%20Track%20Press%20Release%2022SEP2016.pdf. 
  12. ↑ "Nature report describes complete protection after 10 weeks with three doses of PfSPZ- CVac" (Press release). 2017-02-15.
  13. ↑ Butler, Declan (2019-04-16). "Promising malaria vaccine to be tested in first large field trial - The vaccine can confer up to 100% protection and will be tested in 2,100 people on the west African island of Bioko.". Nature. doi:10.1038/d41586-019-01232-4. PMID 32291409. https://www.nature.com/articles/d41586-019-01232-4. Retrieved 2020-08-25. 
  14. ↑ Payne, January (2022-12-26). "2022 News - A Three-Dose Malaria Vaccine Shows Safety, Efficacy in West African Adults". https://www.medschool.umaryland.edu/news/2022/A-Three-Dose-Malaria-Vaccine-Shows-Safety-Efficacy-in-West-African-Adults.html. 
  15. ↑ Sirima, Sodiomon B.; Ouédraogo, Alphonse; Tiono, Alfred B.; Kaboré, Jean M.; Bougouma, Edith C.; Ouattara, Maurice S.; Kargougou, Désiré; Diarra, Amidou et al. (2022-12-07). "A randomized controlled trial showing safety and efficacy of a whole sporozoite vaccine against endemic malaria" (in en). Science Translational Medicine 14 (674): eabj3776. doi:10.1126/scitranslmed.abj3776. ISSN 1946-6234. PMID 36475905. 
  16. ↑ Epstein, J. E.; Tewari, K; Lyke, K. E.; Sim, B. K.; Billingsley, P. F.; Laurens, M. B.; Gunasekera, A; Chakravarty, S et al. (2011). "Live attenuated malaria vaccine designed to protect through hepatic CD8⁺ T cell immunity". Science 334 (6055): 475–80. doi:10.1126/science.1211548. PMID 21903775. Bibcode: 2011Sci...334..475E. https://zenodo.org/record/1230918. 
  17. ↑ Rts, S Clinical Trials; Agnandji, S. T.; Lell, B; Fernandes, J. F.; Abossolo, B. P.; Methogo, B. G.; Kabwende, A. L.; Adegnika, A. A. et al. (2012). "A phase 3 trial of RTS,S/AS01 malaria vaccine in African infants". New England Journal of Medicine 367 (24): 2284–95. doi:10.1056/NEJMoa1208394. PMID 23136909. https://researchonline.lshtm.ac.uk/427472/1/nejmoa1208394.pdf. 
  18. ↑ Seder, R. A.; Chang, L. J.; Enama, M. E.; Zephir, K. L.; Sarwar, U. N.; Gordon, I. J.; Holman, L. A.; James, E. R. et al. (2013). "Protection against malaria by intravenous immunization with a nonreplicating sporozoite vaccine". Science 341 (6152): 1359–65. doi:10.1126/science.1241800. PMID 23929949. Bibcode: 2013Sci...341.1359S. https://zenodo.org/record/1230926. 
  19. ↑ Cockburn, I. A.; Chen, Y. C.; Overstreet, M. G.; Lees, J. R.; Van Rooijen, N; Farber, D. L.; Zavala, F (2010). "Prolonged antigen presentation is required for optimal CD8+ T cell responses against malaria liver stage parasites". PLOS Pathogens 6 (5): e1000877. doi:10.1371/journal.ppat.1000877. PMID 20463809. 
  20. ↑ "Liquid Nitrogen (LN2) Freezers - Isothermal V-Series" (in en-US). https://www.biolifesolutions.com/product-category/freezers/isothermal-v-series/. 
  21. ↑ "MVE Vapor Shipper Series" (in en-US). https://mvebio.com/aluminum-dewars/mve-vapor-shipper-series/. 
  22. ↑ James, Eric R (2021-03-11). "Disrupting vaccine logistics". International Health 13 (3): 211–214. doi:10.1093/inthealth/ihab010. ISSN 1876-3413. PMID 33709112. 
  23. ↑ Research, Center for Biologics Evaluation and (2022-09-19). "ERVEBO" (in en). FDA. https://www.fda.gov/vaccines-blood-biologics/ervebo. 
  24. ↑ Jusu, Morrison O.; Glauser, Geoffrey; Seward, Jane F.; Bawoh, Mohamed; Tempel, Judith; Friend, Michael; Littlefield, Daniel; Lahai, Michael et al. (2018-05-18). "Rapid Establishment of a Cold Chain Capacity of -60°C or Colder for the STRIVE Ebola Vaccine Trial During the Ebola Outbreak in Sierra Leone". The Journal of Infectious Diseases 217 (suppl_1): S48–S55. doi:10.1093/infdis/jix336. ISSN 1537-6613. PMID 29788339. 
  25. ↑ Research, Center for Biologics Evaluation and (2022-08-29). "COMIRNATY" (in en). FDA. https://www.fda.gov/vaccines-blood-biologics/comirnaty. 
  26. ↑ Garcia, Cristina Reyes; Manzi, Fatuma; Tediosi, Fabrizio; Hoffman, Stephen L.; James, Eric R. (2013-01-02). "Comparative cost models of a liquid nitrogen vapor phase (LNVP) cold chain-distributed cryopreserved malaria vaccine vs. a conventional vaccine". Vaccine 31 (2): 380–386. doi:10.1016/j.vaccine.2012.10.109. ISSN 1873-2518. PMID 23146676. 

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