Biology:PrimPol
 Generic protein structure example |
PrimPol is a protein encoded by the PRIMPOL gene in humans.[1][2][3] PrimPol is a eukaryotic protein with both DNA polymerase and DNA Primase activities involved in translesion DNA synthesis. It is the first eukaryotic protein to be identified with priming activity using deoxyribonucleotides.[2][3] It is also the first protein identified in the mitochondria to have translesion DNA synthesis activities.
Etymology
PrimPol was identified in a bioinformatic study and initially presumed to only have primase activity.[4] Subsequent in vitro and in vivo studies have shown it to have both primase and polymerase activities that both localise to the catalytic domain of PrimPol.[2][3][5] For that reason, this protein was assigned the name PrimPol.
Function
PrimPol is a DNA primase and DNA polymerase involved in DNA replication. Unlike the other known DNA polymerases, PrimPol can initiate replication without the need of an RNA primer and can extend from primers produced by PrimPol.[2][3] PrimPol preferentially initiates replication using deoxynucleotides, rather than ribonucleotides and will only extend from a nascent DNA chain using deoxynucleotides. PrimPol exhibits a 1000-fold bias towards Watson-Crick base pairing when extending DNA chains. PrimPol plays an as yet unidentified role in unperturbed replication, PrimPol depleted cells slow replication fork progression, proliferate slower and show an increased RPA foci.[2][3]
Translesion DNA Synthesis
PrimPol is predicted to play a role in translesion DNA synthesis. When the replication fork reaches a site of DNA damage it stalls, which can lead to lethal single stranded gaps and double strand breaks. PrimPol is one of a number of polymerases that can be recruited to replicate past sites of DNA damage. PrimPol localises to chromatin following UV irradiation.[2] PrimPol is able to bypass the highly distortive Pyrimidine dimers produced as a result of UV irradiation of DNA in vitro.[2][3] PrimPol requires its primase activity to bypass UV lesions in vivo without stalling.[5][6] Taken together these data suggest that PrimPol has two separate modes of action to bypass lesions, one in direct read-through of lesions in a classical translesion DNA synthesis manner and one in priming downstream of the lesion and the gap filled in postreplicatively.
In addition to UV lesions, PrimPol is capable of bypassing the 8-Oxoguanine bases that are produced in response to oxidative stress, this is of particular importance in the oxidative environment of the mitochondria.[3] The replicative DNA polymerase identified in the mitochondria, pol γ, deals with these lesions poorly. Furthermore, PrimPol is capable of bypassing an AP site in approximately 80% of cases.[3]
Structure
PrimPol is formed of two protein domains, a catalytic primase-polymerase domain and a zinc finger domain.[2][3] The primase and polymerase catalytic functions of PrimPol localise to the primase-polymerase domain but primase activity of PrimPol requires the zinc finger domain.[5][6]
Subcellular Localization
PrimPol has been found to be mainly located in the cytosol (47%), with large fractions also found in the mitochondria (34%), and nuclear compartments (19%).[3] The mitochondrial fraction of PrimPol is found to be in the matrix of the mitochondria, as opposed to the either the membrane or intermembrane space.
PrimPol mutations
A mutation in the PRIMPOL gene has been correlated with myopia.[7][8] This tyrosine to aspartate (Y89D) mutation has been shown to produce a poorly processive variant of the PrimPol protein, and this Y89D variant impedes replication forks in vivo.[8]
References
- ↑ "Entrez Gene: Entrez Gene: PRIMPOL primase and polymerase (DNA-directed)". https://www.ncbi.nlm.nih.gov/gene/201973.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 "PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA replication". Molecular Cell 52 (4): 566–73. Nov 2014. doi:10.1016/j.molcel.2013.10.035. PMID 24267451.
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 "PrimPol, an archaic primase/polymerase operating in human cells". Molecular Cell 52 (4): 541–53. Nov 2014. doi:10.1016/j.molcel.2013.09.025. PMID 24207056.
- ↑ "Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members.". Nucleic Acids Research 33 (12): 3875–96. Jul 2005. doi:10.1093/nar/gki702. PMID 16027112.
- ↑ 5.0 5.1 5.2 "Molecular dissection of the domain architecture and catalytic activities of human PrimPol". Nucleic Acids Research 42 (9): 5830–45. Mar 2014. doi:10.1093/nar/gku214. PMID 24682820.
- ↑ 6.0 6.1 "Repriming of DNA synthesis at stalled replication forks by human PrimPol". Nature Structural & Molecular Biology 20 (12): 1383–9. Nov 2013. doi:10.1038/nsmb.2719. PMID 24240614.
- ↑ "Exome sequencing reveals CCDC111 mutation associated with high myopia". Human Genetics 132 (8): 913–21. Apr 2013. doi:10.1007/s00439-013-1303-6. PMID 23579484.
- ↑ 8.0 8.1 "Human PrimPol mutation associated with high myopia has a DNA replication defect". Nucleic Acids Research 42 (19): 12102–11. Sep 2014. doi:10.1093/nar/gku879. PMID 25262353.
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