Biology:SRG1 RNA
| SRG1 | |
|---|---|
 Conserved secondary structure of SRG1 RNA | |
| Identifiers | |
| Symbol | SRG1 |
| Rfam | RF01765 |
| Other data | |
| RNA type | Gene; Cis-reg; |
| Domain(s) | Saccharomyces |
| PDB structures | PDBe |
SRG1 RNA (SER3 regulatory gene 1) is a non-coding RNA which represses the expression of SER3 (YER081W).[1][2][3] SER3 is a gene which codes for a phosphoglycerate dehydrogenase involved in the biosynthesis of serine.[4] SRG1 represses SER3 expression via transcription interference, and in that respect is the first intergenic transcript of its kind.[1]
SRG1 was identified when chromatin immunoprecipitation (ChIP) assays showed that in Saccharomyces cerevisiae, even when SER3 was being repressed, TATA-binding protein and RNA Polymerase II were still bound to the SER3 DNA in such a way that should have caused active transcription.[1] Further analysis found a second TATA box 558 bp upstream of SER3. A bioinformatic scan identified this same TATA box element in related Saccharomyces.[5] SRG1 has been found to be cis-acting, it had no repressive effect when in trans.[1]
SRG1 RNA is unrelated to Senescence Related Gene 1 (SRG1), a protein-coding gene found in Arabidopsis thaliana,[6] and is also distinct from Stress Response Gene 1 (SRG1) found in Medicago sativa.[7][8]
References
- ↑ 1.0 1.1 1.2 1.3 "Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene". Nature 429 (6991): 571–574. June 2004. doi:10.1038/nature02538. PMID 15175754. Bibcode: 2004Natur.429..571M.
- ↑ "Entrez Gene SRG1". 2010-07-03. https://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=9164885.
- ↑ "Yeast Genome SRG1". https://www.yeastgenome.org/locus/S000029010.
- ↑ "Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae". The Journal of Biological Chemistry 278 (12): 10264–10272. March 2003. doi:10.1074/jbc.M211692200. PMID 12525494.
- ↑ "Finding functional features in Saccharomyces genomes by phylogenetic footprinting". Science 301 (5629): 71–76. July 2003. doi:10.1126/science.1084337. PMID 12775844. Bibcode: 2003Sci...301...71C.
- ↑ "Uniprot Knowledgebase - Protein SRG1". https://www.uniprot.org/uniprot/Q39224.
- ↑ "Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display". Plant Molecular Biology 33 (4): 737–743. March 1997. doi:10.1023/A:1005728420374. PMID 9132065. http://www.kluweronline.com/art.pdf?issn=0167-4412&volume=33&page=737.
- ↑ "GenBank Accs:U42752.1". 27 May 1997. https://www.ncbi.nlm.nih.gov/nuccore/1136332.
Further reading
- "Transcriptional interference in avian retroviruses—implications for the promoter insertion model of leukaemogenesis". Nature 307 (5948): 241–245. 1984. doi:10.1038/307241a0. PMID 6363938. Bibcode: 1984Natur.307..241C.
- "Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs". Cell 116 (4): 499–509. February 2004. doi:10.1016/S0092-8674(04)00127-8. PMID 14980218.
- "Multiple factors prevent transcriptional interference at the yeast ARO4-HIS7 locus". The Journal of Biological Chemistry 277 (24): 21440–21445. June 2002. doi:10.1074/jbc.M201841200. PMID 11937506.
- "Promoter occlusion: transcription through a promoter may inhibit its activity". Cell 29 (3): 939–944. July 1982. doi:10.1016/0092-8674(82)90456-1. PMID 6217898.
- "Genome-wide analysis of mRNA lengths in Saccharomyces cerevisiae". Genome Biology 5 (1): R2. 2003. doi:10.1186/gb-2003-5-1-r2. PMID 14709174.
External links
 |  |
