Biology:Saos-2 cells
Saos-2 ("sarcoma osteogenic") is a cell line derived from the primary osteosarcoma of an 11-year-old Caucasian girl in 1973 by Fogh et al.[1] The cell line is commonly used in bone cancer research as a model for testing novel therapies.[2] In 1987 Rodan et al. determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules."[3]
Besides their worldwide availability, some of the advantages for using Saos-2 cell line are that they have well-documented characterization data, the possibility to obtain large amounts of cells in short time, and the fact that Saos-2 cells can be fully differentiated in a manner that the osteoblastic cells naturally do.[4] The latter point is described particularly as "the ability of Saos-2 cells to deposit a mineralization-competent extracellular matrix",[5] which makes these cells a valuable model for studying events associated with the late osteoblastic-osteocyte differentiation stage in human cells. [6] [7]
References
- ↑ "One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice". J Natl Cancer Inst 59 (1): 221–226. 1977. doi:10.1093/jnci/59.1.221. PMID 327080. https://academic.oup.com/jnci/article-abstract/59/1/221/888247?redirectedFrom=PDF.
- ↑ "Saos-2". ATCC. https://www.atcc.org/Products/All/HTB-85.aspx.
- ↑ "Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties". Cancer Res. 47 (18): 4961–6. 1987. PMID 3040234.
- ↑ "Phenotypic instability of Saos-2 cells in long-term culture". Biochem. Biophys. Res. Commun. 333 (1): 216–22. 2005. doi:10.1016/j.bbrc.2005.05.097. PMID 15939397.
- ↑ "Matrix deposition by a calcifying human osteogenic sarcoma cell line (SAOS- 2)". Bone 16 (4): 415–26. 1995. doi:10.1016/8756-3282(95)90186-8. PMID 7605701.
- ↑ "The isolation and culture of cells from explants of human trabecular bone". Calcif. Tissue Int. 56 (Suppl 1): S8-10. 1995. doi:10.1007/BF03354640. PMID 7719993.
- ↑ "Saos2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes". Calcif. Tissue Int. 95 (2): 183–193. 2014. doi:10.1007/s00223-014-9879-y. PMID 24916279.
External links
- Information on Saos-2 in the DSMZ catalog
- Information on Saos-2 in the ATCC catalog
- Cellosaurus entry for Saos-2
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