Biology:Osteoblast

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Short description: Cells secreting extracellular matrix
Osteoblast
Bone hypercalcemia - cropped - very high mag.jpg
Osteoblasts (purple) rimming a bony spicule (pink - on diagonal of image). In this routinely fixed and decalcified (bone mineral removed) tissue, the osteoblasts have retracted and are separated from each other and from their underlying matrix. In living bone, the cells are linked by tight junctions and gap junctions, and integrated with underlying osteocytes and matrix H&E stain.
Bone forming cells - Osteoblasts 3 -- Smart-Servier.png
Illustration showing a single osteoblast
Details
LocationBone
FunctionFormation of bone tissue
Identifiers
Greekosteoblast
Anatomical terms of microanatomy

Osteoblasts (from the Greek combining forms for "bone", ὀστέο-, osteo- and βλαστάνω, blastanō "germinate") are cells with a single nucleus that synthesize bone. However, in the process of bone formation, osteoblasts function in groups of connected cells. Individual cells cannot make bone. A group of organized osteoblasts together with the bone made by a unit of cells is usually called the osteon.

Osteoblasts are specialized, terminally differentiated products of mesenchymal stem cells.[1] They synthesize dense, crosslinked collagen and specialized proteins in much smaller quantities, including osteocalcin and osteopontin, which compose the organic matrix of bone.

In organized groups of disconnected cells, osteoblasts produce hydroxyapatite, the bone mineral, that is deposited in a highly regulated manner, into the organic matrix forming a strong and dense mineralized tissue, the mineralized matrix. The mineralized skeleton is the main support for the bodies of air breathing vertebrates. It is also an important store of minerals for physiological homeostasis including both acid-base balance and calcium or phosphate maintenance.[2][3]

Bone structure

The skeleton is a large organ that is formed and degraded throughout life in the air-breathing vertebrates. The skeleton, often referred to as the skeletal system, is important both as a supporting structure and for maintenance of calcium, phosphate, and acid-base status in the whole organism.[4] The functional part of bone, the bone matrix, is entirely extracellular. The bone matrix consists of protein and mineral. The protein forms the organic matrix. It is synthesized and then the mineral is added. The vast majority of the organic matrix is collagen, which provides tensile strength. The matrix is mineralized by deposition of hydroxyapatite (alternative name, hydroxylapatite). This mineral is hard, and provides compressive strength. Thus, the collagen and mineral together are a composite material with excellent tensile and compressive strength, which can bend under a strain and recover its shape without damage. This is called elastic deformation. Forces that exceed the capacity of bone to behave elastically may cause failure, typically bone fractures.[citation needed]

Bone remodeling

Bone is a dynamic tissue that is constantly being reshaped by osteoblasts, which produce and secrete matrix proteins and transport mineral into the matrix, and osteoclasts, which break down the tissues.

Osteoblasts

Osteoblasts are the major cellular component of bone. Osteoblasts arise from mesenchymal stem cells (MSC). MSC give rise to osteoblasts, adipocytes, and myocytes among other cell types. Osteoblast quantity is understood to be inversely proportional to that of marrow adipocytes which comprise marrow adipose tissue (MAT). Osteoblasts are found in large numbers in the periosteum, the thin connective tissue layer on the outside surface of bones, and in the endosteum.

Normally, almost all of the bone matrix, in the air breathing vertebrates, is mineralized by the osteoblasts. Before the organic matrix is mineralized, it is called the osteoid. Osteoblasts buried in the matrix are called osteocytes. During bone formation, the surface layer of osteoblasts consists of cuboidal cells, called active osteoblasts. When the bone-forming unit is not actively synthesizing bone, the surface osteoblasts are flattened and are called inactive osteoblasts. Osteocytes remain alive and are connected by cell processes to a surface layer of osteoblasts. Osteocytes have important functions in skeletal maintenance.

Osteoclasts

Osteoclasts are multinucleated cells that derive from hematopoietic progenitors in the bone marrow which also give rise to monocytes in peripheral blood.[5] Osteoclasts break down bone tissue, and along with osteoblasts and osteocytes form the structural components of bone. In the hollow within bones are many other cell types of the bone marrow. Components that are essential for osteoblast bone formation include mesenchymal stem cells (osteoblast precursor) and blood vessels that supply oxygen and nutrients for bone formation. Bone is a highly vascular tissue, and active formation of blood vessel cells, also from mesenchymal stem cells, is essential to support the metabolic activity of bone. The balance of bone formation and bone resorption tends to be negative with age, particularly in post-menopausal women,[6] often leading to a loss of bone serious enough to cause fractures, which is called osteoporosis.

Osteogenesis

Bone is formed by one of two processes: endochondral ossification or intramembranous ossification. Endochondral ossification is the process of forming bone from cartilage and this is the usual method. This form of bone development is the more complex form: it follows the formation of a first skeleton of cartilage made by chondrocytes, which is then removed and replaced by bone, made by osteoblasts. Intramembranous ossification is the direct ossification of mesenchyme as happens during the formation of the membrane bones of the skull and others.[7]

During osteoblast differentiation, the developing progenitor cells express the regulatory transcription factor Cbfa1/Runx2. A second required transcription factor is Sp7 transcription factor.[8] Osteochondroprogenitor cells differentiate under the influence of growth factors, although isolated mesenchymal stem cells in tissue culture may also form osteoblasts under permissive conditions that include vitamin C and substrates for alkaline phosphatase, a key enzyme that provides high concentrations of phosphate at the mineral deposition site.[1]

Bone morphogenetic proteins

Key growth factors in endochondral skeletal differentiation include bone morphogenetic proteins (BMPs) that determine to a major extent where chondrocyte differentiation occurs and where spaces are left between bones. The system of cartilage replacement by bone has a complex regulatory system. BMP2 also regulates early skeletal patterning. Transforming growth factor beta (TGF-β), is part of a superfamily of proteins that include BMPs, which possess common signaling elements in the TGF beta signaling pathway. TGF-β is particularly important in cartilage differentiation, which generally precedes bone formation for endochondral ossification. An additional family of essential regulatory factors is the fibroblast growth factors (FGFs) that determine where skeletal elements occur in relation to the skin

Steroid and protein hormones

Many other regulatory systems are involved in the transition of cartilage to bone and in bone maintenance. A particularly important bone-targeted hormonal regulator is parathyroid hormone (PTH). Parathyroid hormone is a protein made by the parathyroid gland under the control of serum calcium activity.[3] PTH also has important systemic functions, including to keep serum calcium concentrations nearly constant regardless of calcium intake. Increasing dietary calcium results in minor increases in blood calcium. However, this is not a significant mechanism supporting osteoblast bone formation, except in the condition of low dietary calcium; further, abnormally high dietary calcium raises the risk of serious health consequences not directly related to bone mass including heart attack and stroke.[9] Intermittent PTH stimulation increases osteoblast activity, although PTH is bifunctional and mediates bone matrix degradation at higher concentrations.

The skeleton is also modified for reproduction and in response to nutritional and other hormone stresses; it responds to steroids, including estrogen and glucocorticoids, which are important in reproduction and energy metabolism regulation. Bone turnover involves major expenditures of energy for synthesis and degradation, involving many additional signals including pituitary hormones. Two of these are adrenocorticotropic hormone (ACTH)[10] and follicle stimulating hormone.[11] The physiological role for responses to these, and several other glycoprotein hormones, is not fully understood, although it is likely that ACTH is bifunctional, like PTH, supporting bone formation with periodic spikes of ACTH, but causing bone destruction in large concentrations. In mice, mutations that reduce the efficiency of ACTH-induced glucocorticoid production in the adrenals cause the skeleton to become dense (osteosclerotic bone).[12][13]

Organization and ultrastructure

In well-preserved bone studied at high magnification via electron microscopy, individual osteoblasts are shown to be connected by tight junctions, which prevent extracellular fluid passage and thus create a bone compartment separate from the general extracellular fluid.[14] The osteoblasts are also connected by gap junctions, small pores that connect osteoblasts, allowing the cells in one cohort to function as a unit.[15] The gap junctions also connect deeper layers of cells to the surface layer (osteocytes when surrounded by bone). This was demonstrated directly by injecting low molecular weight fluorescent dyes into osteoblasts and showing that the dye diffused to surrounding and deeper cells in the bone-forming unit.[16] Bone is composed of many of these units, which are separated by impermeable zones with no cellular connections, called cement lines.

Collagen and accessory proteins

Almost all of the organic (non-mineral) component of bone is dense collagen type I,[17] which forms dense crosslinked ropes that give bone its tensile strength. By mechanisms still unclear, osteoblasts secrete layers of oriented collagen, with the layers parallel to the long axis of the bone alternating with layers at right angles to the long axis of the bone every few micrometers. Defects in collagen type I cause the commonest inherited disorder of bone, called osteogenesis imperfecta.[18]

Minor, but important, amounts of small proteins, including osteocalcin and osteopontin, are secreted in bone's organic matrix.[19] Osteocalcin is not expressed at significant concentrations except in bone, and thus osteocalcin is a specific marker for bone matrix synthesis.[20] These proteins link organic and mineral component of bone matrix.[21] The proteins are necessary for maximal matrix strength due to their intermediate localization between mineral and collagen.

However, in mice where expression of osteocalcin or osteopontin was eliminated by targeted disruption of the respective genes (knockout mice), accumulation of mineral was not notably affected, indicating that organization of matrix is not significantly related to mineral transport.[22][23]

Bone versus cartilage

The primitive skeleton is cartilage, a solid avascular (without blood vessels) tissue in which individual cartilage-matrix secreting cells, or chondrocytes, occur. Chondrocytes do not have intercellular connections and are not coordinated in units. Cartilage is composed of a network of collagen type II held in tension by water-absorbing proteins, hydrophilic proteoglycans.[24] This is the adult skeleton in cartilaginous fishes such as sharks. It develops as the initial skeleton in more advanced classes of animals.

In air-breathing vertebrates, cartilage is replaced by cellular bone. A transitional tissue is mineralized cartilage. Cartilage mineralizes by massive expression of phosphate-producing enzymes, which cause high local concentrations of calcium and phosphate that precipitate.[24] This mineralized cartilage is not dense or strong. In the air breathing vertebrates it is used as a scaffold for formation of cellular bone made by osteoblasts, and then it is removed by osteoclasts, which specialize in degrading mineralized tissue.

Osteoblasts produce an advanced type of bone matrix consisting of dense, irregular crystals of hydroxyapatite, packed around the collagen ropes.[25] This is a strong composite material that allows the skeleton to be shaped mainly as hollow tubes. Reducing the long bones to tubes reduces weight while maintaining strength.

Mineralization of bone

The mechanisms of mineralization are not fully understood. Fluorescent, low-molecular weight compounds such as tetracycline or calcein bind strongly to bone mineral, when administered for short periods. They then accumulate in narrow bands in the new bone.[26] These bands run across the contiguous group of bone-forming osteoblasts. They occur at a narrow (sub-micrometer) mineralization front. Most bone surfaces express no new bone formation, no tetracycline uptake and no mineral formation. This strongly suggests that facilitated or active transport, coordinated across the bone-forming group, is involved in bone formation, and that only cell-mediated mineral formation occurs. That is, dietary calcium does not create mineral by mass action.

The mechanism of mineral formation in bone is clearly distinct from the phylogenetically older process by which cartilage is mineralized: tetracycline does not label mineralized cartilage at narrow bands or in specific sites, but diffusely, in keeping with a passive mineralization mechanism.[25]

Osteoblasts separate bone from the extracellular fluid by tight junctions [14] by regulated transport. Unlike in cartilage, phosphate and calcium cannot move in or out by passive diffusion, because the tight osteoblast junctions isolate the bone formation space. Calcium is transported across osteoblasts by facilitated transport (that is, by passive transporters, which do not pump calcium against a gradient).[25] In contrast, phosphate is actively produced by a combination of secretion of phosphate-containing compounds, including ATP, and by phosphatases that cleave phosphate to create a high phosphate concentration at the mineralization front. Alkaline phosphatase is a membrane-anchored protein that is a characteristic marker expressed in large amounts at the apical (secretory) face of active osteoblasts.

Major features of the bone-forming complex, the osteon, composed of osteoblasts and osteocytes.

At least one more regulated transport process is involved. The stoichiometry of bone mineral basically is that of hydroxyapatite precipitating from phosphate, calcium, and water at a slightly alkaline pH:[27]

6 HPO2−
4 + 2 H
2O + 10 Ca2+ ⇌ Ca
10(PO
4)
6(OH)
2 + 8 H+

In a closed system as mineral precipitates, acid accumulates, rapidly lowering the pH and stopping further precipitation. Cartilage presents no barrier to diffusion and acid therefore diffuses away, allowing precipitation to continue. In the osteon, where matrix is separated from extracellular fluid by tight junctions, this cannot occur. In the controlled, sealed compartment, removing H+ drives precipitation under a wide variety of extracellular conditions, as long as calcium and phosphate are available in the matrix compartment.[28] The mechanism by which acid transits the barrier layer remains uncertain. Osteoblasts have capacity for Na+/H+ exchange via the redundant Na/H exchangers, NHE1 and NHE6.[29] This H+ exchange is a major element in acid removal, although the mechanism by which H+ is transported from the matrix space into the barrier osteoblast is not known.

In bone removal, a reverse transport mechanism uses acid delivered to the mineralized matrix to drive hydroxyapatite into solution.[30]

Osteocyte feedback

Feedback from physical activity maintains bone mass, while feedback from osteocytes limits the size of the bone-forming unit.[31][32][33] An important additional mechanism is secretion by osteocytes, buried in the matrix, of sclerostin, a protein that inhibits a pathway that maintains osteoblast activity. Thus, when the osteon reaches a limiting size, it deactivates bone synthesis.[34]

Morphology and histological staining

Hematoxylin and eosin staining (H&E) shows that the cytoplasm of active osteoblasts is slightly basophilic due to the substantial presence of rough endoplasmic reticulum. The active osteoblast produces substantial collagen type I. About 10% of the bone matrix is collagen with the balance mineral.[27] The osteoblast's nucleus is spherical and large. An active osteoblast is characterized morphologically by a prominent Golgi apparatus that appears histologically as a clear zone adjacent to the nucleus. The products of the cell are mostly for transport into the osteoid, the non-mineralized matrix. Active osteoblasts can be labeled by antibodies to Type-I collagen, or using naphthol phosphate and the diazonium dye fast blue to demonstrate alkaline phosphatase enzyme activity directly.

  • Osteoblast (Wright Giemsa stain, 100x)

  • Light micrograph of decalcified cancellous bone displaying osteoblasts actively synthesizing osteoid, containing two osteocytes.

  • Light micrograph of undecalcified tissue displaying osteoblasts actively synthesizing osteoid (center).

  • Light micrograph of undecalcified tissue displaying osteoblasts actively synthesizing rudimentary bone tissue (center).

  • Osteoblasts lining bone (H&E stain).

Isolation of Osteoblasts

  1. The first isolation technique by microdissection method was originally described by Fell et al.[35] using chick limb bones which were separated into periosteum and remaining parts. She obtained cells which possessed osteogenic characteristics from cultured tissue using chick limb bones which were separated into periosteum and remaining parts. She obtained cells which possessed osteogenic characteristics from cultured tissue.
  2. Enzymatic digestion is one of the most advanced techniques for isolating bone cell populations and obtaining osteoblasts. Peck et al. (1964)[36] described the original method that is now often used by many researchers.
  3. In 1974 Jones et al.[37] found that osteoblasts moved laterally in vivo and in vitro under different experimental conditions and escribed the migration method in detail. The osteoblasts were, however, contaminated by cells migrating from the vascular openings, which might include endothelial cells and fibroblasts.

See also

  • List of human cell types derived from the germ layers
  • List of distinct cell types in the adult human body

References

  1. ↑ 1.0 1.1 "Multilineage potential of adult human mesenchymal stem cells". Science 284 (5411): 143–7. April 1999. doi:10.1126/science.284.5411.143. PMID 10102814. Bibcode: 1999Sci...284..143P. 
  2. ↑ "Regulation of bone cell function by acid-base balance". Proc Nutr Soc 62 (2): 511–20. 2003. doi:10.1079/pns2003268. PMID 14506899. 
  3. ↑ 3.0 3.1 "The developmental basis of skeletal cell differentiation and the molecular basis of major skeletal defects". Biol Rev Camb Philos Soc 83 (4): 401–15. November 2008. doi:10.1111/j.1469-185X.2008.00048.x. PMID 18710437. 
  4. ↑ "Balanced regulation of proliferation, growth, differentiation, and degradation in skeletal cells". Ann. N. Y. Acad. Sci. 1116 (1): 165–73. November 2007. doi:10.1196/annals.1402.029. PMID 17646258. Bibcode: 2007NYASA1116..165B. 
  5. ↑ Loutit, J.F.; Nisbet, N.W. (January 1982). "The Origin of Osteoclasts". Immunobiology 161 (3–4): 193–203. doi:10.1016/S0171-2985(82)80074-0. PMID 7047369. 
  6. ↑ "Reproductive hormones and bone". Curr Osteoporos Rep 8 (2): 60–7. June 2010. doi:10.1007/s11914-010-0014-3. PMID 20425612. 
  7. ↑ Larsen, William J. (2001). Human embryology (3. ed.). Philadelphia, Pa.: Churchill Livingstone. pp. 355–357. ISBN 0-443-06583-7. https://archive.org/details/humanembryology0003lars. 
  8. ↑ "Transcriptional control of skeletogenesis". Annu Rev Genom Hum Genet 9: 183–96. 2008. doi:10.1146/annurev.genom.9.081307.164437. PMID 18767962. 
  9. ↑ "Cardiovascular complications of calcium supplements". J. Cell. Biochem. 116 (4): 494–501. April 2015. doi:10.1002/jcb.25028. PMID 25491763. 
  10. ↑ "ACTH protects against glucocorticoid-induced osteonecrosis of bone". Proc. Natl. Acad. Sci. U.S.A. 107 (19): 8782–7. May 2010. doi:10.1073/pnas.0912176107. PMID 20421485. Bibcode: 2010PNAS..107.8782Z. 
  11. ↑ "FSH directly regulates bone mass". Cell 125 (2): 247–60. April 2006. doi:10.1016/j.cell.2006.01.051. PMID 16630814. 
  12. ↑ "Absence of HDL cholesteryl ester uptake in mice via SR-BI impairs an adequate adrenal glucocorticoid-mediated stress response to fasting". J. Lipid Res. 49 (4): 738–45. April 2008. doi:10.1194/jlr.M700475-JLR200. PMID 18204096. 
  13. ↑ "The atherogenic Scarb1 null mouse model shows a high bone mass phenotype". Am. J. Physiol. Endocrinol. Metab. 306 (1): E48–57. January 2014. doi:10.1152/ajpendo.00421.2013. PMID 24253048. 
  14. ↑ 14.0 14.1 "Junctions between early developing osteoblasts of rat calvaria as revealed by freeze-fracture and ultrathin section electron microscopy". Arch. Histol. Cytol. 58 (3): 285–92. August 1995. doi:10.1679/aohc.58.285. PMID 8527235. 
  15. ↑ "Morphological evidence of gap junctions between bone cells". Calcif. Tissue Int. 33 (5): 509–12. 1981. doi:10.1007/BF02409482. PMID 6797704. 
  16. ↑ "Functional gap junctions between osteocytic and osteoblastic cells". J. Bone Miner. Res. 15 (2): 209–17. February 2000. doi:10.1359/jbmr.2000.15.2.209. PMID 10703922. 
  17. ↑ "Transitions in collagen types during matrix-induced cartilage, bone, and bone marrow formation". Proc. Natl. Acad. Sci. U.S.A. 74 (12): 5589–92. December 1977. doi:10.1073/pnas.74.12.5589. PMID 271986. Bibcode: 1977PNAS...74.5589R. 
  18. ↑ "Mutations in collagen genes: causes of rare and some common diseases in humans". FASEB J. 5 (7): 2052–60. April 1991. doi:10.1096/fasebj.5.7.2010058. PMID 2010058. 
  19. ↑ "Osteoblast and chondroblast differentiation". Bone 17 (2 Suppl): 77S–83S. August 1995. doi:10.1016/8756-3282(95)00183-E. PMID 8579903. 
  20. ↑ "[Osteocalcin (or bone gla-protein), a new biological marker for studying bone pathology]" (in fr). Presse Med 15 (14): 643–6. April 1986. PMID 2939433. 
  21. ↑ "Why does bone matrix contain non-collagenous proteins? The possible roles of osteocalcin, osteonectin, osteopontin and bone sialoprotein in bone mineralisation and resorption". Cell Biol. Int. 18 (6): 617–28. June 1994. doi:10.1006/cbir.1994.1088. PMID 8075622. 
  22. ↑ "Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin". Bone 23 (3): 187–96. September 1998. doi:10.1016/s8756-3282(98)00092-1. PMID 9737340. 
  23. ↑ "Osteopontin deficiency increases bone fragility but preserves bone mass". Bone 46 (6): 1564–73. June 2010. doi:10.1016/j.bone.2010.02.014. PMID 20171304. 
  24. ↑ 24.0 24.1 "Mechanisms balancing skeletal matrix synthesis and degradation". Biochem. J. 364 (Pt 2): 329–41. June 2002. doi:10.1042/BJ20020165. PMID 12023876. 
  25. ↑ 25.0 25.1 25.2 "Calcium and bone disease". BioFactors 37 (3): 159–67. 2011. doi:10.1002/biof.143. PMID 21674636. 
  26. ↑ "Tetracycline-based histological analysis of bone remodeling". Calcif Tissue Res 3 (1): 211–37. 1969. doi:10.1007/BF02058664. PMID 4894738. 
  27. ↑ 27.0 27.1 The Chemical Dynamics of Bone Mineral. University of Chicago Press. 1958-01-01. ISBN 0-226-57512-8. https://archive.org/details/in.ernet.dli.2015.550358. [page needed]
  28. ↑ "Concerning pH gradients between the extracellular compartment and fluids bathing the bone mineral surface and their relation to calcium ion distribution". J. Clin. Invest. 41 (5): 1163–8. May 1962. doi:10.1172/JCI104569. PMID 14498063. 
  29. ↑ "High capacity Na+/H+ exchange activity in mineralizing osteoblasts". J. Cell. Physiol. 226 (6): 1702–12. June 2011. doi:10.1002/jcp.22501. PMID 21413028. 
  30. ↑ "Osteoclastic bone resorption by a polarized vacuolar proton pump". Science 245 (4920): 855–7. August 1989. doi:10.1126/science.2528207. PMID 2528207. Bibcode: 1989Sci...245..855B. 
  31. ↑ "Osteocyte and bone structure". Curr Osteoporos Rep 1 (1): 5–10. June 2003. doi:10.1007/s11914-003-0002-y. PMID 16036059. 
  32. ↑ Dance, Amber (23 February 2022). "Fun facts about bones: More than just scaffolding". Knowable Magazine. doi:10.1146/knowable-022222-1. https://knowablemagazine.org/article/health-disease/2022/fun-facts-about-bones-more-just-scaffolding. Retrieved 8 March 2022. 
  33. ↑ Robling, Alexander G.; Bonewald, Lynda F. (10 February 2020). "The Osteocyte: New Insights". Annual Review of Physiology 82 (1): 485–506. doi:10.1146/annurev-physiol-021119-034332. ISSN 0066-4278. PMID 32040934. PMC 8274561. https://doi.org/10.1146/annurev-physiol-021119-034332. Retrieved 8 March 2022. 
  34. ↑ Baron, Roland; Rawadi, Georges; Roman‐Roman, Sergio (2006). "WNT Signaling: A Key Regulator of Bone Mass". Current Topics in Developmental Biology. 76. pp. 103–127. doi:10.1016/S0070-2153(06)76004-5. ISBN 978-0-12-153176-8. 
  35. ↑ Fell, HB (January 1932). "The Osteogenic Capacity in vitro of Periosteum and Endosteum Isolated from the Limb Skeleton of Fowl Embryos and Young Chicks.". Journal of Anatomy 66 (Pt 2): 157–180.11. PMID 17104365. 
  36. ↑ Peck, W. A.; Birge, S. J.; Fedak, S. A. (11 December 1964). "Bone Cells: Biochemical and Biological Studies after Enzymatic Isolation". Science 146 (3650): 1476–1477. doi:10.1126/science.146.3650.1476. PMID 14208576. Bibcode: 1964Sci...146.1476P. 
  37. ↑ Jones, S.J.; Boyde, A. (December 1977). "Some morphological observations on osteoclasts". Cell and Tissue Research 185 (3): 387–97. doi:10.1007/bf00220298. PMID 597853. 

Further reading

  • William F. Neuman and Margaret W. Neuman. (1958). The Chemical Dynamics of Bone Mineral. Chicago: The University of Chicago Press. ISBN:0-226-57512-8.
  • Netter, Frank H. (1987). Musculoskeletal system: anatomy, physiology, and metabolic disorders. Summit, New Jersey: Ciba-Geigy Corporation ISBN:0-914168-88-6.

External links



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