Biology:Smart ligand

From HandWiki
Short description: Sub-type of substance that forms a complex with a biomolecule

Smart ligands are affinity ligands selected with pre-defined equilibrium (Kd), kinetic (koff, kon) and thermodynamic (ΔH, ΔS) parameters of biomolecular interaction.

Ligands with desired parameters can be selected from large combinatorial libraries of biopolymers using instrumental separation techniques with well-described kinetic behaviour, such as kinetic capillary electrophoresis (KCE), surface plasmon resonance (SPR), microscale thermophoresis (MST),[1][2] etc. Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed.

Smart ligands can find a set of unique applications in biomedical research, drug discovery and proteomic studies. For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide dynamic range of measured concentrations.

References

  1. ↑ "Optical Thermophoresis quantifies Buffer dependence of Aptamer Binding". Angew. Chem. Int. Ed. 49 (12): 2238–41. Feb 2010. doi:10.1002/anie.200903998. PMID 20186894. 
  2. ↑ "Protein-binding assays in biological liquids using microscale thermophoresis". Nature Communications 1 (7): 100. Oct 2010. doi:10.1038/ncomms1093. PMID 20981028. Bibcode: 2010NatCo...1..100W. 
  • "Selection of smart small-molecule ligands: the proof of principle". Anal. Chem. 81 (1): 490–4. January 2009. doi:10.1021/ac8023813. PMID 19055427. 
  • "Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM)". J. Am. Chem. Soc. 127 (32): 11224–5. August 2005. doi:10.1021/ja0530016. PMID 16089434. 
  • "Selection of smart aptamers by methods of kinetic capillary electrophoresis". Anal. Chem. 78 (9): 3171–8. May 2006. doi:10.1021/ac060144h. PMID 16643010. 
  • "Smart aptamers facilitate multi-probe affinity analysis of proteins with ultra-wide dynamic range of measured concentrations". J. Am. Chem. Soc. 129 (23): 7260–1. June 2007. doi:10.1021/ja072269p. PMID 17503828. 
  • Krylov SN (January 2007). "Kinetic CE: foundation for homogeneous kinetic affinity methods". Electrophoresis 28 (1–2): 69–88. doi:10.1002/elps.200600577. PMID 17245689. 



Retrieved from "https://handwiki.org/wiki/index.php?title=Biology:Smart_ligand&oldid=380725"