Biology:Aptamer

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Short description: Oligonucleotide or peptide molecules that bind specific targets
Left: Unbound aptamer. Right: the aptamer bound to its target protein. The protein is in yellow. Parts of the aptamer that change shape when it binds its target are in blue, while the unchanging parts are in orange. The parts of the aptamer that contact the protein are highlighted in red.
Breast cancer cells incubated with aptamers that bind selectively to biomarkers on the cancer cells, but not to healthy cells. Aptamers are linked to Alexa Fluor 594, a molecule that glows red under UV light. This type of test allows a doctor or researcher to identify cancer cells in a tissue sample from a patient.

Aptamers are short sequences of artificial DNA, RNA, XNA, or peptide that bind a specific target molecule, or family of target molecules. They exhibit a range of affinities (KD in the pM to μM range),[1][2] with variable levels of off-target binding[3] and are sometimes classified as chemical antibodies. Aptamers and antibodies can be used in many of the same applications, but the nucleic acid-based structure of aptamers, which are mostly oligonucleotides, is very different from the amino acid-based structure of antibodies, which are proteins. This difference can make aptamers a better choice than antibodies for some purposes (see antibody replacement).

Aptamers are used in biological lab research and medical tests. If multiple aptamers are combined into a single assay, they can measure large numbers of different proteins in a sample. They can be used to identify molecular markers of disease, or can function as drugs, drug delivery systems and controlled drug release systems. They also find use in other molecular engineering tasks.

Most aptamers originate from SELEX, a family of test-tube experiments for finding useful aptamers in a massive pool of different DNA sequences. This process is much like natural selection, directed evolution or artificial selection. In SELEX, the researcher repeatedly selects for the best aptamers from a starting DNA library made of about a quadrillion different randomly generated pieces of DNA or RNA. After SELEX, the researcher might mutate or change the chemistry of the aptamers and do another selection, or might use rational design processes to engineer improvements. Non-SELEX methods for discovering aptamers also exist.

Researchers optimize aptamers to achieve a variety of beneficial features. The most important feature is specific and sensitive binding to the chosen target. When aptamers are exposed to bodily fluids, as in serum tests or aptamer therapeutics, it is often important for them to resist digestion by DNA- and RNA-destroying proteins. Therapeutic aptamers often must be modified to clear slowly from the body. Aptamers that change their shape dramatically when they bind their target are useful as molecular switches to turn a sensor on and off. Some aptamers are engineered to fit into a biosensor or in a test of a biological sample. It can be useful in some cases for the aptamer to accomplish a pre-defined level or speed of binding. As the yield of the synthesis used to produce known aptamers shrinks quickly for longer sequences,[4] researchers often truncate aptamers to the minimal binding sequence to reduce the production cost.

Etymology

The word "aptamer" is a neologism coined by Andrew Ellington and Jack Szostak in their first publication on the topic. They did not provide a precise definition, stating "We have termed these individual RNA sequences 'aptamers', from the Latin 'aptus', to fit."[5]

Classification

A typical aptamer is a synthetically generated ligand exploiting the combinatorial diversity of DNA, RNA, XNA, or peptide to achieve strong, specific binding for a particular target molecule or family of target molecules. Aptamers are occasionally classified as "chemical antibodies" or "antibody mimics".[6] However, most aptamers are small, with a molecular weight of 6-30 kDa, in contrast to the 150 kDa size of antibodies, and contain one binding site rather than the two matching antigen binding regions of a typical antibody.

History

Jack Szostak, Nobel laureate and one of the inventors of SELEX and aptamers.

Since its first application in 1967,[7] directed evolution methodologies have been used to develop biomolecules with new properties and functions. Early examples include the modification of the bacteriophage Qbeta replication system and the generation of ribozymes with modified cleavage activity.[8]

In 1990, two teams independently developed and published SELEX (Systematic Evolution of Ligands by EXponential enrichment) methods and generated RNA aptamers: the lab of Larry Gold, using the term SELEX for their process of selecting RNA ligands against T4 DNA polymerase[9] and the lab of Jack Szostak, selecting RNA ligands against various organic dyes.[5][10] Two years later, the Szostak lab and Gilead Sciences, acting independently of one another, used in vitro selection schemes to generate DNA aptamers for organic dyes[11] and human thrombin,[12] respectively. In 2001, SELEX was automated by J. Colin Cox in the Ellington lab, reducing the duration of a weeks-long selection experiment to just three days.[13][14][15]

In 2002, two groups led by Ronald Breaker and Evgeny Nudler published the first definitive evidence for a riboswitch, a nucleic acid-based genetic regulatory element, the existence of which had previously been suspected. Riboswitches possess similar molecular recognition properties to aptamers. This discovery added support to the RNA World hypothesis, a postulated stage in time in the origin of life on Earth.[16]

Properties

Structure

The complex and diverse secondary and tertiary structure of aptamers, as shown in this schematic of an aptamer's secondary structure, is what lets them bind their target strongly and specifically. Complementary base pairing is visible in the black lines connecting some G-C and A-T bases. This is a feature of nucleic acids that does not exist in the amino acids of antibodies. It helps aptamers form these unique structures. Hairpin regions (red), which rely on this base pairing, enhance the aptamer's stability at different temperatures. This image also shows examples of chemical modifications to the base aptamer. Two unnatural bases, which enhance durability, are in yellow. The biotin molecule binds with extreme strength to streptavidin, allowing the aptamer to be anchored to other molecules or to a surface in sensors and assays.

Most aptamers are based on a specific oligomer sequence of 20-100 bases and 3-20 kDa. Some have chemical modifications for functional enhancements or compatibility with larger engineered molecular systems. DNA, RNA, XNA, and peptide aptamer chemistries can each offer distinct profiles in terms of shelf stability, durability in serum or in vivo, specificity and sensitivity, cost, ease of generation, amplification, and characterization, and familiarity to users. Typically, DNA- and RNA-based aptamers exhibit low immunogenicity, are amplifiable via Polymerase Chain Reaction (PCR), and have complex secondary structure and tertiary structure.[17][18][19][20] DNA- and XNA-based aptamers exhibit superior shelf stability. XNA-based aptamers can introduce additional chemical diversity to increase binding affinity or greater durability in serum or in vivo.

As 22 genetically-encoded and over 500 naturally-occurring amino acids exist, peptide aptamers, as well as antibodies, have much greater potential combinatorial diversity per unit length relative to the 4 nucleic acids in DNA or RNA.[21] Chemical modifications of nucleic acid bases or backbones increase the chemical diversity of standard nucleic acid bases.[22]

Split aptamers are composed of two or more DNA strands that are pieces of a larger parent aptamer that has been broken in two by a molecular nick.[23] The ability of each component strand to bind targets will depend on the location of the nick, as well as the secondary structures of the daughter strands.[24] The presence of a target molecule supports the DNA fragments joining together. This can be used as the basis for biosensors.[25] Once assembled, the two separate DNA strands can be ligated into a single strand.

Unmodified aptamers are cleared rapidly from the bloodstream, with a half-life of seconds to hours. This is mainly due to nuclease degradation, which physically destroys the aptamers, as well as clearance by the kidneys, a result of the aptamer's low molecular weight and size. Several modifications, such as 2'-fluorine-substituted pyrimidines and polyethylene glycol (PEG) linkage, permit a serum half-life of days to weeks. PEGylation can add sufficient mass and size to prevent clearance by the kidneys in vivo. Unmodified aptamers can treat coagulation disorders. The problem of clearance and nuclease digestion is diminished when they are applied to the eye, where there is a lower concentration of nuclease and the rate of clearance is lower.[26] Rapid clearance from serum can also be useful in some applications, such as in vivo diagnostic imaging.[27]

In a study on aptamers[28] designed to bind with proteins associated with Ebola infection, a comparison was made among three aptamers isolated for their ability to bind the target protein EBOV sGP. Although these aptamers vary in both sequence and structure, they exhibit remarkably similar relative affinities for sGP from EBOV and SUDV, as well as EBOV GP1.2. Notably, these aptamers demonstrated a high degree of specificity for the GP gene products. One aptamer, in particular, proved effective as a recognition element in an electrochemical sensor, enabling the detection of sGP and GP1.2 in solution, as well as GP1.2 within a membrane context.The results of this research point to the intriguing possibility that certain regions on protein surfaces may possess aptatropic qualities. Identifying the key features of such sites, in conjunction with improved 3-D structural predictions for aptamers, holds the potential to enhance the accuracy of predicting aptamer interaction sites on proteins. This, in turn, may help identify aptamers with a heightened likelihood of binding proteins with high affinity, as well as shed light on protein mutations that could significantly impact aptamer binding.This comprehensive understanding of the structure-based interactions between aptamers and proteins is vital for refining the computational predictability of aptamer-protein binding. Moreover, it has the potential to eventually eliminate the need for the experimental SELEX protocol.

Targets

Aptamer targets can include small molecules and heavy metal ions, larger ligands such as proteins, and even whole cells.[29][30] These targets include lysozyme,[31] thrombin,[32][33] human immunodeficiency virus trans-acting responsive element (HIV TAR),[34] hemin,[35] interferon γ,[36] vascular endothelial growth factor (VEGF),[37][38] prostate specific antigen (PSA),[39][40] dopamine,[41] and the non-classical oncogene, heat shock factor 1 (HSF1).[42]

Aptamers have been generated against cancer cells,[43] prions,[44] bacteria,[45] and viruses. Viral targets of aptamers include influenza A and B viruses,[46] Respiratory syncytial virus (RSV),[46] SARS coronavirus (SARS-CoV)[46] and SARS-CoV-2.[47]

Aptamers may be particularly useful for environmental science proteomics.[48] Antibodies, like other proteins, are more difficult to sequence than nucleic acids. They are also costly to maintain and produce, and are at constant risk of contamination, as they are produced via cell culture or are harvested from animal serum. For this reason, researchers interested in little-studied proteins and species may find that companies will not produce, maintain, or adequately validate the quality of antibodies against their target of interest.[49] By contrast, aptamers are simple to sequence and cost nothing to maintain, as their exact structure can be stored digitally and synthesized on demand. This may make them more economically feasible as research tools for underfunded biological research subjects. Aptamers exist for plant compounds, such as theophylline (found in tea)[50] and abscisic acid (a plant immune hormone).[51] An aptamer against a-amanitin (the toxin that causes lethal Amanita poisoning) has been developed, an example of an aptamer against a mushroom target.[52]

Aptamer applications can be roughly grouped into sensing, therapeutic, reagent production, and engineering categories. Sensing applications are important in environmental, biomedical, epidemiological, biosecurity, and basic research applications, where aptamers act as probes in assays, imaging methods, diagnostic assays, and biosensors.[29][53][54][55][56][57] In therapeutic applications and precision medicine, aptamers can function as drugs,[58] as targeted drug delivery vehicles,[59] as controlled release mechanisms, and as reagents for drug discovery via high-throughput screening for small molecules[60] and proteins.[61][62] Aptamers have application for protein production monitoring, quality control, and purification.[63][64][65] They can function in molecular engineering applications as a way to modify proteins, such as enhancing DNA polymerase to make PCR more reliable.[66][67][68][69]

Because the affinity of the aptamer also affects its dynamic range and limit of detection, aptamers with a lower affinity may be desirable when assaying high concentrations of a target molecule.[70] Affinity chromatography also depends on the ability of the affinity reagent, such as an aptamer, to bind and release its target, and lower affinities may aid in the release of the target molecule.[71] Hence, specific applications determine the useful range for aptamer affinity.

Antibody replacement

Aptamers can replace antibodies in many biotechnology applications.[72][49] In laboratory research and clinical diagnostics, they can be used in aptamer-based versions of immunoassays including enzyme-linked immunosorbent assay (ELISA),[73] western blot,[74] immunohistochemistry (IHC),[75] and flow cytometry.[76] As therapeutics, they can function as agonists or antagonists of their ligand.[77] While antibodies are a familiar technology with a well-developed market, aptamers are a relatively new technology to most researchers, and aptamers have been generated against only a fraction of important research targets.[78] Unlike antibodies, unmodified aptamers are more susceptible to nuclease digestion in serum and renal clearance in vivo. Aptamers are much smaller in size and mass than antibodies, which could be a relevant factor in choosing which is best suited for a given application. When aptamers are available for a particular application, their advantages over antibodies include potentially lower immunogenicity, greater replicability and lower cost, a greater level of control due to the in vitro selection conditions, and capacity to be efficiently engineered for durability, specificity, and sensitivity.[79]

In addition, aptamers contribute to reduction of research animal use.[80] While antibodies often rely on animals for initial discovery, as well as for production in the case of polyclonal antibodies, both the selection and production of aptamers is typically animal-free. However, phage display methods allow for selection of antibodies in vitro, followed by production from a monoclonal cell line, avoiding the use of animals entirely.[81]

Controlled release of therapeutics

The ability of aptamers to reversibly bind molecules such as proteins has generated increasing interest in using them to facilitate controlled release of therapeutic biomolecules, such as growth factors. This can be accomplished by tuning the binding strength to passively release the growth factors,[82] along with active release via mechanisms such as hybridization of the aptamer with complementary oligonucleotides[83] or unfolding of the aptamer due to cellular traction forces.[84]

AptaBiD

AptaBiD (Aptamer-Facilitated Biomarker Discovery) is an aptamer-based method for biomarker discovery.[85]

Peptide Aptamers

While most aptamers are based on DNA, RNA, or XNA, peptide aptamers[86] are artificial proteins selected or engineered to bind specific target molecules.

Structure

Peptide aptamers consist of one or more peptide loops of variable sequence displayed by a protein scaffold. Derivatives known as tadpoles, in which peptide aptamer "heads" are covalently linked to unique sequence double-stranded DNA "tails", allow quantification of scarce target molecules in mixtures by PCR (using, for example, the quantitative real-time polymerase chain reaction) of their DNA tails.[87] The peptides that form the aptamer variable regions are synthesized as part of the same polypeptide chain as the scaffold and are constrained at their N and C termini by linkage to it. This double structural constraint decreases the diversity of the 3D structures that the variable regions can adopt,[88] and this reduction in structural diversity lowers the entropic cost of molecular binding when interaction with the target causes the variable regions to adopt a uniform structure.

Selection

The most common peptide aptamer selection system is the yeast two-hybrid system. Peptide aptamers can also be selected from combinatorial peptide libraries constructed by phage display and other surface display technologies such as mRNA display, ribosome display, bacterial display and yeast display. These experimental procedures are also known as biopanning. All the peptides panned from combinatorial peptide libraries have been stored in the MimoDB database.[89][90]

Applications

Libraries of peptide aptamers have been used as "mutagens", in studies in which an investigator introduces a library that expresses different peptide aptamers into a cell population, selects for a desired phenotype, and identifies those aptamers that cause the phenotype. The investigator then uses those aptamers as baits, for example in yeast two-hybrid screens to identify the cellular proteins targeted by those aptamers. Such experiments identify particular proteins bound by the aptamers, and protein interactions that the aptamers disrupt, to cause the phenotype.[91][92] In addition, peptide aptamers derivatized with appropriate functional moieties can cause specific post-translational modification of their target proteins, or change the subcellular localization of the targets.[93]

This assay tests the ability of two different types of aptamers (V and I) to detect their respective protein targets (VEGF and IFN-y). The labels Apt1, Apt2, Apt3, and Apt4 are in decreasing order of binding strength (i.e. Apt1 is the strongest aptamer). The DD, AD, DA, and AA letters mean that they have different combinations of unnatural base pairs. This causes their difference in binding strengths. The "-" columns have no protein, and the "+" columns do have protein. Aptamer with protein (+) and without protein (-) is loaded into wells in a gel and moves down the column lanes. If target is present, they bind and travel more slowly, due to the charge on the aptamer and the mass of the protein. Otherwise, the unbound aptamer moves quickly to the end of the lane. The difference in position between the "+" and "-" bands is the "mobility shift." This allows the researcher to detect the presence or absence of the protein. The darker band in the leftmost V and I lanes show that stronger aptamer-target binding makes it easier to detect the target at a given amount of target protein in the sample. The bottom image includes denaturing urea in the gel that disrupts aptamer-target binding in the weaker I aptamers, showing that the aptamer-protein binding is indeed what caused the mobility shift.

Industry and Research Community

Commercial products and companies based on aptamers include the drug Macugen (pegaptanib)[94] and the clinical diagnostic company SomaLogic.[95] The International Society on Aptamers (INSOAP), a professional society for the aptamer research community, publishes a journal devoted to the topic, Aptamers. Apta-index[96] is a current database cataloging and simplifying the ordering process for over 700 aptamers.

See also


References

  1. ↑ Rhodes, Andrew; Smithers, Nick; Chapman, Trevor; Parsons, Sarah; Rees, Stephen (2001-10-05). "The generation and characterisation of antagonist RNA aptamers to MCP-1" (in en). FEBS Letters 506 (2): 85–90. doi:10.1016/S0014-5793(01)02895-2. ISSN 0014-5793. PMID 11591377. https://www.sciencedirect.com/science/article/pii/S0014579301028952. 
  2. ↑ Stoltenburg, Regina; Nikolaus, Nadia; Strehlitz, Beate (2012-12-30). "Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics" (in en). Journal of Analytical Methods in Chemistry 2012: e415697. doi:10.1155/2012/415697. ISSN 2090-8865. PMID 23326761. 
  3. ↑ "Aptamers, antibody scFv, and antibody Fab' fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements". Biosensors & Bioelectronics 85: 32–45. November 2016. doi:10.1016/j.bios.2016.04.091. PMID 27155114. 
  4. ↑ "DNA Oligonucleotide Synthesis". Millipore Sigma. https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/dna-oligonucleotide-synthesis. 
  5. ↑ 5.0 5.1 "In vitro selection of RNA molecules that bind specific ligands". Nature 346 (6287): 818–822. August 1990. doi:10.1038/346818a0. PMID 1697402. Bibcode: 1990Natur.346..818E. 
  6. ↑ "Aptamers: A promising chemical antibody for cancer therapy". Oncotarget 7 (12): 13446–13463. March 2016. doi:10.18632/oncotarget.7178. PMID 26863567. 
  7. ↑ "An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule". Proceedings of the National Academy of Sciences of the United States of America 58 (1): 217–224. July 1967. doi:10.1073/pnas.58.1.217. PMID 5231602. Bibcode: 1967PNAS...58..217M. 
  8. ↑ "Amplification, mutation and selection of catalytic RNA". Gene 82 (1): 83–87. October 1989. doi:10.1016/0378-1119(89)90033-4. PMID 2684778. 
  9. ↑ "Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase". Science 249 (4968): 505–510. August 1990. doi:10.1126/science.2200121. PMID 2200121. Bibcode: 1990Sci...249..505T. 
  10. ↑ "SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands". Biomolecular Engineering 24 (4): 381–403. October 2007. doi:10.1016/j.bioeng.2007.06.001. PMID 17627883. 
  11. ↑ "Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures". Nature 355 (6363): 850–852. February 1992. doi:10.1038/355850a0. PMID 1538766. Bibcode: 1992Natur.355..850E. 
  12. ↑ "Selection of single-stranded DNA molecules that bind and inhibit human thrombin". Nature 355 (6360): 564–566. February 1992. doi:10.1038/355564a0. PMID 1741036. Bibcode: 1992Natur.355..564B. 
  13. ↑ "Automated selection of anti-protein aptamers". Bioorganic & Medicinal Chemistry 9 (10): 2525–2531. October 2001. doi:10.1016/s0968-0896(01)00028-1. PMID 11557339. 
  14. ↑ "Automated acquisition of aptamer sequences". Combinatorial Chemistry & High Throughput Screening 5 (4): 289–299. June 2002. doi:10.2174/1386207023330291. PMID 12052180. 
  15. ↑ "Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer". Nucleic Acids Research 30 (20): 108e–108. October 2002. doi:10.1093/nar/gnf107. PMID 12384610. 
  16. ↑ "Riboswitches and the RNA world". Cold Spring Harbor Perspectives in Biology 4 (2): a003566. February 2012. doi:10.1101/cshperspect.a003566. PMID 21106649. 
  17. ↑ "Selection of Anti-gluten DNA Aptamers in a Deep Eutectic Solvent". Angewandte Chemie 57 (39): 12850–12854. September 2018. doi:10.1002/ange.201804860. PMID 30070419. Bibcode: 2018AngCh.13013032S. 
  18. ↑ "Defining the secondary structural requirements of a cocaine-binding aptamer by a thermodynamic and mutation study". Biophysical Chemistry 153 (1): 9–16. December 2010. doi:10.1016/j.bpc.2010.09.009. PMID 21035241. 
  19. ↑ "2.8 A crystal structure of the malachite green aptamer". Journal of Molecular Biology 301 (1): 117–128. August 2000. doi:10.1006/jmbi.2000.3951. PMID 10926496. 
  20. ↑ "Structural Investigations of RNA and DNA aptamers in Solution". Journal of Cellular Biochemistry 59: 13–81. 1995. doi:10.1002/jcb.240590703. 
  21. ↑ "Nucleic acid and peptide aptamers: fundamentals and bioanalytical aspects". Angewandte Chemie 51 (6): 1316–1332. February 2012. doi:10.1002/anie.201006630. PMID 22213382. 
  22. ↑ "In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies". RNA Biology 13 (12): 1232–1245. December 2016. doi:10.1080/15476286.2016.1236173. PMID 27715478. 
  23. ↑ "Split aptamers and their applications in sandwich aptasensors". TrAC Trends in Analytical Chemistry 80: 581–593. 2016. doi:10.1016/j.trac.2016.04.006. https://www.sciencedirect.com/science/article/pii/S0165993615301989. 
  24. ↑ "General approach for engineering small-molecule-binding DNA split aptamers". Analytical Chemistry 85 (20): 9916–9923. October 2013. doi:10.1021/ac402500n. PMID 24033257. 
  25. ↑ "Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors". Nucleic Acids Research 48 (7): 3400–3422. April 2020. doi:10.1093/nar/gkaa132. PMID 32112111. 
  26. ↑ "Fit for the Eye: Aptamers in Ocular Disorders". Nucleic Acid Therapeutics 26 (3): 127–146. June 2016. doi:10.1089/nat.2015.0573. PMID 26757406. 
  27. ↑ "Current progress of aptamer-based molecular imaging". Journal of Nuclear Medicine 55 (3): 353–356. March 2014. doi:10.2967/jnumed.113.126144. PMID 24525205. 
  28. ↑ Banerjee, S.; Hemmat, M.A.; Shubham, S.; Gosai, A.; Devarakonda, S.; Jiang, N.; Geekiyanage, C.; Dillard, J.A.; Maury, W.; Shrotriya, P.; et al. Structurally Different Yet Functionally Similar: Aptamers Specific for the Ebola Virus Soluble Glycoprotein and GP1,2 and Their Application in Electrochemical Sensing. Int. J. Mol. Sci. 2023, 24, 4627. https://doi.org/10.3390/ijms24054627
  29. ↑ 29.0 29.1 "Nanomaterial based aptasensors for clinical and environmental diagnostic applications". Nanoscale Advances 1 (6): 2123–2138. June 2019. doi:10.1039/C9NA00153K. PMID 36131986. Bibcode: 2019NanoA...1.2123K. 
  30. ↑ "Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens". Molecules 22 (2): 215. January 2017. doi:10.3390/molecules22020215. PMID 28146093. 
  31. ↑ "Biophysical characterization of DNA aptamer interactions with vascular endothelial growth factor". Biopolymers 91 (2): 145–156. February 2009. doi:10.1002/bip.21097. PMID 19025993. 
  32. ↑ "Crystal structure of an RNA aptamer bound to thrombin". RNA 14 (12): 2504–2512. December 2008. doi:10.1261/rna.1239308. PMID 18971322. 
  33. ↑ Kohn, Eric M.; Konovalov, Kirill; Gomez, Christian A.; Hoover, Gillian N.; Yik, Andrew Kai-hei; Huang, Xuhui; Martell, Jeffrey D. (2023-08-02). "Terminal Alkyne-Modified DNA Aptamers with Enhanced Protein Binding Affinities" (in en). ACS Chemical Biology. doi:10.1021/acschembio.3c00183. ISSN 1554-8929. PMID 37531184. https://pubs.acs.org/doi/10.1021/acschembio.3c00183. 
  34. ↑ "Aptamers targeted to an RNA hairpin show improved specificity compared to that of complementary oligonucleotides". Biochemistry 45 (39): 12076–12082. October 2006. doi:10.1021/bi0606344. PMID 17002307. 
  35. ↑ "Direct In Vitro Selection of Hemin-Binding DNA Aptamer with Peroxidase Activity". Bulletin of the Chemical Society of Japan 82: 99–104. 2009. doi:10.1246/bcsj.82.99. 
  36. ↑ "A simple and direct electrochemical detection of interferon-gamma using its RNA and DNA aptamers". Biosensors & Bioelectronics 23 (12): 1819–1824. July 2008. doi:10.1016/j.bios.2008.02.021. PMID 18406597. 
  37. ↑ "Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease". Nature Reviews. Drug Discovery 5 (2): 123–132. February 2006. doi:10.1038/nrd1955. PMID 16518379. 
  38. ↑ Moghadam, Fatemeh Mortazavi; Rahaie, Mahdi (May 2019). "A signal-on nanobiosensor for VEGF165 detection based on supraparticle copper nanoclusters formed on bivalent aptamer" (in en). Biosensors and Bioelectronics 132: 186–195. doi:10.1016/j.bios.2019.02.046. PMID 30875630. https://linkinghub.elsevier.com/retrieve/pii/S0956566319301630. 
  39. ↑ "Selection of DNA aptamer against prostate specific antigen using a genetic algorithm and application to sensing". Biosensors & Bioelectronics 26 (4): 1386–1391. December 2010. doi:10.1016/j.bios.2010.07.057. PMID 20692149. 
  40. ↑ "Selection of RNA aptamers specific to active prostate-specific antigen". Biotechnology Letters 32 (3): 379–385. March 2010. doi:10.1007/s10529-009-0168-1. PMID 19943183. 
  41. ↑ "Retention of function in the DNA homolog of the RNA dopamine aptamer". Biochemical and Biophysical Research Communications 388 (4): 732–735. October 2009. doi:10.1016/j.bbrc.2009.08.084. PMID 19699181. 
  42. ↑ "Inhibiting heat shock factor 1 in human cancer cells with a potent RNA aptamer". PLOS ONE 9 (5): e96330. 2014. doi:10.1371/journal.pone.0096330. PMID 24800749. Bibcode: 2014PLoSO...996330S. 
  43. ↑ "Nanoparticle-aptamer bioconjugates for cancer targeting". Expert Opinion on Drug Delivery 3 (3): 311–324. May 2006. doi:10.1517/17425247.3.3.311. PMID 16640493. 
  44. ↑ "Prion-protein-specific aptamer reduces PrPSc formation". ChemBioChem 3 (8): 717–725. August 2002. doi:10.1002/1439-7633(20020802)3:8<717::AID-CBIC717>3.0.CO;2-C. PMID 12203970. 
  45. ↑ "Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detection". Biosensors & Bioelectronics 98: 486–493. December 2017. doi:10.1016/j.bios.2017.07.004. PMID 28728009. 
  46. ↑ 46.0 46.1 46.2 "Advancements in Nucleic Acid Based Therapeutics against Respiratory Viral Infections". Journal of Clinical Medicine 8 (1): 6. December 2018. doi:10.3390/jcm8010006. PMID 30577479. 
  47. ↑ "A SARS-CoV-2 Spike Binding DNA Aptamer that Inhibits Pseudovirus Infection by an RBD-Independent Mechanism*". Angewandte Chemie 60 (18): 10279–10285. April 2021. doi:10.1002/anie.202100316. PMID 33683787. 
  48. ↑ "Antibodies, Nanobodies, or Aptamers-Which Is Best for Deciphering the Proteomes of Non-Model Species?". International Journal of Molecular Sciences 21 (7): 2485. April 2020. doi:10.3390/ijms21072485. PMID 32260091. 
  49. ↑ 49.0 49.1 "Anything You Can Do, I Can Do Better: Can Aptamers Replace Antibodies in Clinical Diagnostic Applications?". Molecules 24 (23): 4377. November 2019. doi:10.3390/molecules24234377. PMID 31801185. 
  50. ↑ "An aptamer nanopore-enabled microsensor for detection of theophylline". Biosensors & Bioelectronics 105: 36–41. May 2018. doi:10.1016/j.bios.2018.01.016. PMID 29351868. 
  51. ↑ "Detection of plant hormone abscisic acid (ABA) using an optical aptamer-based sensor with a microfluidics capillary interface". 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). 2017. pp. 370–373. doi:10.1109/MEMSYS.2017.7863418. ISBN 978-1-5090-5078-9. https://ieeexplore.ieee.org/document/7863418. 
  52. ↑ "Selection and analysis of a DNA aptamer binding α-amanitin from Amanita phalloides". Acta Biochimica Polonica 64 (3): 401–406. 2017. doi:10.18388/abp.2017_1615. PMID 28787470. 
  53. ↑ "Commercialization of an aptamer-based diagnostic test". July 2012. https://neoaptamers.com/wp-content/uploads/2021/01/Penner2012IVDTech31commercializationaptamer-baseddiagkits-1.pdf. 
  54. ↑ "Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes". Chemical Communications (36): 3735–3737. September 2007. doi:10.1039/B707642H. PMID 17851611. 
  55. ↑ "Functional Nucleic Acid Probe for Parallel Monitoring K(+) and Protoporphyrin IX in Living Organisms". Analytical Chemistry 88 (5): 2937–2943. March 2016. doi:10.1021/acs.analchem.5b04936. PMID 26866998. 
  56. ↑ "Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets". Nature Chemistry 3 (9): 697–703. July 2011. doi:10.1038/nchem.1092. PMID 21860458. Bibcode: 2011NatCh...3..697X. 
  57. ↑ Agnivo Gosai, Brendan Shin Hau Yeah, Marit Nilsen-Hamilton, Pranav Shrotriya, "Label free thrombin detection in presence of high concentration of albumin using an aptamer-functionalized nanoporous membrane", Biosensors and Bioelectronics, Volume 126, 2019, pp. 88–95, ISSN 0956-5663, doi:10.1016/j.bios.2018.10.010.
  58. ↑ "Aptamers: Novel Therapeutics and Potential Role in Neuro-Oncology". Cancers 12 (10): 2889. October 2020. doi:10.3390/cancers12102889. PMID 33050158. 
  59. ↑ "Aptamers in Therapeutics and Drug Delivery". Advanced Drug Delivery Reviews 134: 1–2. September 2018. doi:10.1016/j.addr.2018.11.001. PMID 30442313. 
  60. ↑ "Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization". Nature Protocols 3 (4): 579–587. 2008. doi:10.1038/nprot.2008.15. PMID 18388939. 
  61. ↑ "Integrating DNA Nanotechnology with Aptamers for Biological and Biomedical Applications" (in en). Matter 4 (2): 461–489. 2021-02-03. doi:10.1016/j.matt.2020.11.002. ISSN 2590-2385. 
  62. ↑ "Sensing with Nanopores and Aptamers: A Way Forward". Sensors 20 (16): 4495. August 2020. doi:10.3390/s20164495. PMID 32796729. Bibcode: 2020Senso..20.4495R. 
  63. ↑ "Application of aptamers in detection and chromatographic purification of antibiotics in different matrices". TrAC Trends in Analytical Chemistry 95: 1–22. 2017. doi:10.1016/j.trac.2017.07.023. https://www.sciencedirect.com/science/article/pii/S0165993617301516. Retrieved 4 July 2022. 
  64. ↑ "An improved method for the in vitro evolution of aptamers and applications in protein detection and purification". Nucleic Acids Research 31 (18): 110e–110. September 2003. doi:10.1093/nar/gng110. PMID 12954786. 
  65. ↑ "Aptamers as Versatile Molecular Tools for Antibody Production Monitoring and Quality Control". Journal of the American Chemical Society 142 (28): 12079–12086. July 2020. doi:10.1021/jacs.9b13370. PMID 32516525. 
  66. ↑ "DNA-assisted site-selective protein modification". Biopolymers 113 (3): e23483. March 2022. doi:10.1002/bip.23483. PMID 34878181. 
  67. ↑ "Sensitivity and specificity of photoaptamer probes". Molecular & Cellular Proteomics 2 (1): 11–18. January 2003. doi:10.1074/mcp.m200059-mcp200. PMID 12601078. 
  68. ↑ "Aptamer-based affinity labeling of proteins". Angewandte Chemie 51 (36): 9176–9180. September 2012. doi:10.1002/anie.201204174. PMID 22865679. 
  69. ↑ "Site-selective and inducible acylation of thrombin using aptamer-catalyst conjugates". Chemical Communications 57 (96): 12960–12963. December 2021. doi:10.1039/d1cc05446e. PMID 34792071. 
  70. ↑ Wilson, Brandon D.; Soh, H. Tom (2020-08-01). "Re-Evaluating the Conventional Wisdom about Binding Assays" (in en). Trends in Biochemical Sciences 45 (8): 639–649. doi:10.1016/j.tibs.2020.04.005. ISSN 0968-0004. PMID 32402748. 
  71. ↑ Janson, Jan-Christer (2012-01-03) (in en). Protein Purification: Principles, High Resolution Methods, and Applications. John Wiley & Sons. ISBN 978-1-118-00219-3. https://books.google.com/books?id=2RCbtWQJ2SsC&dq=protein+purification+second+edition+principles+high+resolution+methods&pg=PR7. 
  72. ↑ "Replacing antibodies with aptamers in lateral flow immunoassay". Biosensors & Bioelectronics 71: 230–242. September 2015. doi:10.1016/j.bios.2015.04.041. PMID 25912679. 
  73. ↑ "Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay". Biosensors & Bioelectronics 64: 392–403. February 2015. doi:10.1016/j.bios.2014.09.026. PMID 25278480. 
  74. ↑ "Aptamer "Western" blotting for E. coli outer membrane proteins and key virulence factors in pathogenic E. coli serotypes". Aptamers and Synthetic Antibodies. 2016. http://researcherslinks.com/current-issues/Measuring-Predictive-Performance-Data-Mining-Artificial-Neural-Akkaraman-Sheep%20/20/1/234/html. 
  75. ↑ "The Application of Aptamers for Immunohistochemistry". Nucleic Acid Therapeutics 26 (3): 120–126. June 2016. doi:10.1089/nat.2015.0569. PMID 26862683. 
  76. ↑ "Aptamers: versatile probes for flow cytometry". Applied Microbiology and Biotechnology 97 (16): 7097–7109. August 2013. doi:10.1007/s00253-013-5070-z. PMID 23838792. 
  77. ↑ "Aptamers as targeted therapeutics: current potential and challenges". Nature Reviews. Drug Discovery 16 (3): 181–202. March 2017. doi:10.1038/nrd.2016.199. PMID 27807347. 
  78. ↑ "Predicting the Uncertain Future of Aptamer-Based Diagnostics and Therapeutics". Molecules 20 (4): 6866–6887. April 2015. doi:10.3390/molecules20046866. PMID 25913927. 
  79. ↑ "Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development". Biotechnology Advances 37 (1): 28–50. 2019. doi:10.1016/j.biotechadv.2018.11.001. PMID 30408510. 
  80. ↑ "A multi-faceted approach to achieving the global acceptance of animal-free research methods". Alternatives to Laboratory Animals 44 (5): 495–498. October 2016. doi:10.1177/026119291604400511. PMID 27805832. 
  81. ↑ "Phage Display Derived Monoclonal Antibodies: From Bench to Bedside". Frontiers in Immunology 11: 1986. 2020. doi:10.3389/fimmu.2020.01986. PMID 32983137. 
  82. ↑ "Hydrogel functionalization with DNA aptamers for sustained PDGF-BB release". Chemical Communications 46 (11): 1857–1859. March 2010. doi:10.1039/B924909E. PMID 20198232. 
  83. ↑ "Programmable release of multiple protein drugs from aptamer-functionalized hydrogels via nucleic acid hybridization". Journal of the American Chemical Society 134 (30): 12410–12413. August 2012. doi:10.1021/ja305238a. PMID 22816442. 
  84. ↑ "Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces". Advanced Materials 31 (7): e1806380. February 2019. doi:10.1002/adma.201806380. PMID 30614086. Bibcode: 2019AdM....3106380S. 
  85. ↑ "Aptamer-facilitated biomarker discovery (AptaBiD)". Journal of the American Chemical Society 130 (28): 9137–9143. July 2008. doi:10.1021/ja801951p. PMID 18558676. 
  86. ↑ "Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2". Nature 380 (6574): 548–550. April 1996. doi:10.1038/380548a0. PMID 8606778. Bibcode: 1996Natur.380..548C. 
  87. ↑ "Tadpoles by the tail". Nature Methods 2 (1): 11–12. January 2005. doi:10.1038/nmeth0105-11. PMID 15782163. 
  88. ↑ "Coupling of local folding to site-specific binding of proteins to DNA". Science 263 (5148): 777–784. February 1994. doi:10.1126/science.8303294. PMID 8303294. Bibcode: 1994Sci...263..777S. 
  89. ↑ "MimoDB 2.0: a mimotope database and beyond". Nucleic Acids Research 40 (Database issue): D271–D277. January 2012. doi:10.1093/nar/gkr922. PMID 22053087. 
  90. ↑ "MimoDB: a mimotope database and beyond". http://immunet.cn/mimodb. 
  91. ↑ ""Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections". Proceedings of the National Academy of Sciences of the United States of America 96 (15): 8567–8572. July 1999. doi:10.1073/pnas.96.15.8567. PMID 10411916. Bibcode: 1999PNAS...96.8567G. 
  92. ↑ "Calcineurin A versus NS5A-TP2/HD domain containing 2: a case study of site-directed low-frequency random mutagenesis for dissecting target specificity of peptide aptamers". Molecular & Cellular Proteomics 12 (7): 1939–1952. July 2013. doi:10.1074/mcp.M112.024612. PMID 23579184. 
  93. ↑ "Targeted modification and transportation of cellular proteins". Proceedings of the National Academy of Sciences of the United States of America 97 (25): 13720–13725. December 2000. doi:10.1073/pnas.97.25.13720. PMID 11106396. Bibcode: 2000PNAS...9713720C. 
  94. ↑ "FDA Approves Eyetech/Pfizer's Macugen". https://www.reviewofophthalmology.com/article/fda-approves-eyetechpfizers-macugen. 
  95. ↑ "SomaLogic and Illumina Combine Strengths to Propel Innovation in Proteomics". https://www.biospace.com/article/somalogic-and-illumina-combine-strengths-to-propel-innovation-in-proteomics-/. 
  96. ↑ "Apta-Index™ (Aptamer Database) - Library of 500+ Aptamers" (in en-US). https://www.aptagen.com/apta-index/. 

Further reading



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