Chemistry:ANNINE-6plus
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| Preferred IUPAC name
3-[10-(Dibutylamino)chryseno[2,1-f]isoquinolin-2-ium-2-yl]-N,N,N-trimethylpropan-1-aminium dibromide | |
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3D model (JSmol)
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| Properties | |
| C39H47Br2N3 | |
| Molar mass | 717.634 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
ANNINE-6plus is a water soluble voltage sensitive dye (also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany.[1] It is used to optically measure the changes in transmembrane voltage of excitable cells, including neurons, skeletal and cardiac myocytes.
Voltage sensitivity
ANNINE-6plus has a fractional fluorescent intensity change (ΔF/F per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm).
Applications
ANNINE-6plus has been applied in the microscopic imaging of action potentials of cardiomyocyte in perfused mice heart.[2] Using confocal microscopy in conjunction with ANNINE-6plus, single sweep action potentials with high peak signal-to-noise ratio (SNR) have been recorded from single transverse tubule (t-tubule) of a few micrometers in the ventricular cardiomyocyte.
References
- ↑ Peter Fromherz (2008). "ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity". European Biophysics Journal 37 (4): 509–514. doi:10.1007/s00249-007-0210-y. PMID 17687549.
- ↑ Guixue Bu (2009). "Uniform action potential repolarization within the sarcolemma of in situ ventricular cardiomyocytes". Biophysical Journal 96 (6): 2532–2546. doi:10.1016/j.bpj.2008.12.3896. PMID 19289075. Bibcode: 2009BpJ....96.2532B.
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