Chemistry:Chelex 100
Chelex 100 is a chelating material from Bio-Rad[1] used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions. It is a styrene-divinylbenzene co-polymer containing iminodiacetic acid groups.
A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups.
Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including Mg2+, which is an essential cofactor for DNases. Chelex protects the sample from DNases that might remain active after the boiling and could subsequently degrade the DNA, rendering it unsuitable for PCR. After boiling, the Chelex-DNA preparation is stable and can be stored at 4°C for 3–4 months.[2] Polar resin beads bind polar cellular components after breaking open cells, while DNA and RNA remain in water solution above the Chelex resin.
However, the heating steps do denature the double helix, and the resulting single-stranded DNA is less stable in storage.
References
- ↑ "Chelex® 100 and Chelex 20 Chelating Ion Exchange Resin Instruction Manual". http://www.bio-rad.com/webmaster/pdfs/9184_Chelex.PDF.
- ↑ Walsh, P.S., Metzger D.A., and Higuchi, R. (1991). "Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material". BioTechniques 10 (4): 506–513. PMID 1867860.
- Daniel Harris. Quantitative Chemical Analysis, seventh edition, 2007. ISBN:0-7167-7041-5. Page 594.
- R. N. Ceo; M. R. Kazerouni; K. Rengan (1993). "Sorption of silver ions by Chelex 100 chelating resin". Journal of Radioanalytical and Nuclear Chemistry 172 (1): 43–48. doi:10.1007/BF02040660.
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