Chemistry:Pyrylium-1
Pyrylium-1 (Py-1) is a fluorogenic salt of a pyrylium derivative and tetrafluoroborate. It is an amine-labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products.[1] It is within the "chameleon labels" class,[2] so named due to their clear color-changing properties upon conjugation. Py-1 was first reported in 2004.[3] It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a high sensitivity via laser-induced fluorescence.[1] Once bound to protein the excitation wavelength is 503 nm (green) and the emission wavelength is 603 nm (orange).[1] Similar to FQ, these fluorescence wavelengths makes Py-1 suitable for excitation with a 488 nm argon-ion laser.
Reaction
File:Py-1 reaction scheme.svg
Post conjugation, the Py-1 adduct (an addition of C21H21N) adds about 287.1674 Da to the target molecule.
See also
- FQ
- CBQCA
- Fluorescamine
References
- ↑ 1.0 1.1 1.2 Craig, Douglas B.; Wetzl, Bianca K.; Duerkop, Axel; Wolfbeis, Otto S. (2005). "Determination of picomolar concentrations of proteins using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection". Electrophoresis (Wiley) 26 (11): 2208–2213. doi:10.1002/elps.200410332. ISSN 0173-0835. PMID 15880625.
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedWolfbeis2021 - ↑ Wetzl, Bianca K.; Yarmoluk, Sergiy M.; Craig, Douglas B.; Wolfbeis, Otto S. (2004-10-11). "Chameleon Labels for Staining and Quantifying Proteins". Angewandte Chemie International Edition (Wiley) 43 (40): 5400–5402. doi:10.1002/anie.200460508. ISSN 1433-7851. PMID 15468079.
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