Chemistry:(3-(2-Furoyl)-quinoline-2 carboxaldehyde)
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Short description: Fluorogenic amine labelling dye
-quinoline-2_carboxaldehyde)_chemical_structure.svg)
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| Names | |
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| IUPAC name
3-(furan-2-carbonyl)quinoline-2-carbaldehyde
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| Other names
FQ, (3-(2-furoyl)-quinoline-2 carboxaldehyde), ATTO-TAG FQ, FQCA
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| Identifiers | |
3D model (JSmol)
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PubChem CID
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| Properties | |
| C15H9NO3 | |
| Molar mass | 251.24 g/mol |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
3-(2-furoyl)-quinoline-2-carboxaldehyde (FQ) is a fluorogenic amine labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products.[1] It was first reported in 1990.[2] Cyanide, typically provided via KCN or NaCN salts, is a required co-substrate in the fluorogenic reaction.[3] It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a silver stain-like high sensitivity via laser-induced fluorescence.[4] Once bound to protein the excitation wavelength is 480 nm (blue) and the emission wavelength is ~600 nm (orange).[5]
Reaction
See also
- CBQCA
- Fluorescamine
- Py-1
References
- ↑ Stoyanov, Alexander V.; Ahmadzadeh, Hossein; Krylov, Sergey N. (2002-11-25). "Heterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde". Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 780 (2): 283–287. doi:10.1016/s1570-0232(02)00535-4. ISSN 1570-0232. PMID 12401353. https://pubmed.ncbi.nlm.nih.gov/12401353/.
- ↑ Beale, Stephen C.; Hsieh, You-Zung; Wiesler, Donald; Novotny, Milos (1990). "Application of 3-(2-furoyl)quinoline-2-carbaldehyde as a fluorogenic reagent for the analysis of primary amines by liquid chromatography with laser-induced fluorescence detection". Journal of Chromatography A (Elsevier BV) 499: 579–587. doi:10.1016/s0021-9673(00)97002-x. ISSN 0021-9673. PMID 2324216.
- ↑ Fisher Scientific, Thermo. "ATTO-TAG CBQCA and ATTO-TAG FQ - Product Information". Thermo Fisher Scientific. https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2Fmp02333.pdf.
- ↑ Arrell, Miriam S.; Kálmán, Franka (2016-10-19). "Estimation of protein concentration at high sensitivity using SDS-capillary gel electrophoresis-laser induced fluorescence detection with 3-(2-furoyl)quinoline-2-carboxaldehyde protein labeling". Electrophoresis (Wiley) 37 (22): 2913–2921. doi:10.1002/elps.201600246. ISSN 0173-0835. PMID 27570211.
- ↑ "3-(2-Furoyl)quinoline-2-carboxaldehyde". https://www.sigmaaldrich.com/US/en/product/sigma/17884.
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