Overlay assay
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An overlay assay is a biological technique used to find proteins that bind to a protein of interest. It begins with proteins that are dispersed but fixed (e.g. a tissue slice or run on a membrane by gel electrophoresis) and then incubated in the labelled protein of interest. The label will indicate the location of proteins that bind to the protein of interest.
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Original source: https://en.wikipedia.org/wiki/Overlay assay.
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