Biology:Potato dextrose agar: Difference between revisions
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{{Short description|Microbial growth medium}} | {{Short description|Microbial growth medium}} | ||
[[File:Pdamasterplate.jpg|thumb|Master plate of bacterial soil isolates growing on potato dextrose agar. ]] | [[File:Pdamasterplate.jpg|thumb|Master plate of bacterial soil isolates growing on potato dextrose agar. ]] | ||
[[File:Aspergillus sp. plate.JPG|alt=|thumb|200x200px|''[[ | {{Use mdy dates|date = February 2019}} | ||
[[File:Aspergillus sp. plate.JPG|alt=|thumb|200x200px|''[[Aspergillus]] sp.'' growing in potato dextrose agar]] | |||
'''Potato dextrose agar''' (BAM Media M127<ref name="bamm127">[https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm063519.htm BAM Media M127: Potato Dextrose Agar] from the U.S. [[Organization:Food and Drug Administration|Food and Drug Administration]]</ref>) and '''potato dextrose broth''' are common microbiological growth media made from [[Biology:Potato|potato]] infusion and [[Chemistry:Glucose|dextrose]]. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria.<ref>{{cite web| author=Harold Eddleman, Ph. D| title= Making Bacteria Media from Potato| date=February 1998| url= http://www.disknet.com/indiana_biolab/b029.htm| publisher= disknet.com | work=Indiana Biolab | access-date= 2011-03-04 | archive-url=https://web.archive.org/web/20110716090823/http://www.disknet.com/indiana_biolab/b029.htm | archive-date=2011-07-16 |url-status=dead}}</ref> | '''Potato dextrose agar''' (BAM Media M127<ref name="bamm127">[https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm063519.htm BAM Media M127: Potato Dextrose Agar] from the U.S. [[Organization:Food and Drug Administration|Food and Drug Administration]]</ref>) and '''potato dextrose broth''' are common microbiological growth media made from [[Biology:Potato|potato]] infusion and [[Chemistry:Glucose|dextrose]]. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria.<ref>{{cite web| author=Harold Eddleman, Ph. D| title= Making Bacteria Media from Potato| date=February 1998| url= http://www.disknet.com/indiana_biolab/b029.htm| publisher= disknet.com | work=Indiana Biolab | access-date= 2011-03-04 | archive-url=https://web.archive.org/web/20110716090823/http://www.disknet.com/indiana_biolab/b029.htm | archive-date=2011-07-16 |url-status=dead}}</ref> | ||
PDA has the capability to culture various bacteria and fungi found in the soil. This agar can be | PDA has the capability to culture various bacteria and fungi found in the soil. This agar can be acidified or loaded with [[Chemistry:Antibiotic|antibiotic]]s such as [[Chemistry:Chloramphenicol|chloramphenicol]] to inhibit unwanted bacterial growth. PDA is used in the food industry to test for fungi that can spoil food products. It is also used in the pharmaceutical industry to screen compound libraries for potential [[Chemistry:Antifungal|antifungal]] agents.<ref>{{Cite book |last=Pitt & Hocking |title=Fungi and food spoilage |year=2009 |location=Springer Science & Business Media|bibcode=2009ffs..book.....P }}</ref> | ||
Potato dextrose agar is a versatile growing medium for bacteria and fungi (yeasts and molds). This agar is used for a broad range of fungi but there are other agars that are more selective for specific types of fungi. These agars include but are not limited to malt extract agar and sabouraud agar. Malt extract agar is more acidic than PDA and is commonly used to cultivate penicillium species.<ref>{{Cite journal |date=2009 |title=Atlas of clinical fungi |journal=Centraalbureau voor Schimmelcultures |issue=2}}</ref> Sabouraud agar is also slightly | Potato dextrose agar is a versatile growing medium for bacteria and fungi (yeasts and molds). This agar is used for a broad range of fungi but there are other agars that are more selective for specific types of fungi. These agars include but are not limited to malt extract agar and sabouraud agar. Malt extract agar is more acidic than PDA and is commonly used to cultivate [[Biology:Penicillium|penicillium]] species.<ref>{{Cite journal |date=2009 |title=Atlas of clinical fungi |journal=Centraalbureau voor Schimmelcultures |issue=2}}</ref> Sabouraud agar is also slightly acidic with pH 5.6-6.0 which is similar to PDA. It is most often used for the isolation of pathogenic fungi such as [[Biology:Dermatophyte|dermatophytes]]. | ||
== Typical composition == | == Typical composition == | ||
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|| 1000 mL || water | || 1000 mL || water | ||
|- | |- | ||
|| | || (strained broth from 200 g of infused potato into the water above) || potatoes<br /><small>(sliced washed unpeeled)</small> | ||
|- | |- | ||
|| 20 g || dextrose | || 20 g || dextrose | ||
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* Atlas, R.M.: ''Handbook of Microbiological Media'', second edition. Lawrence C. Parks (1997) | * Atlas, R.M.: ''Handbook of Microbiological Media'', second edition. Lawrence C. Parks (1997) | ||
{{Growth media}} | |||
[[Category:Potatoes]] | [[Category:Potatoes]] | ||
{{Sourceattribution|Potato dextrose agar}} | {{Sourceattribution|Potato dextrose agar}} | ||
Latest revision as of 22:38, 8 May 2025
Potato dextrose agar (BAM Media M127[1]) and potato dextrose broth are common microbiological growth media made from potato infusion and dextrose. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria.[2]
PDA has the capability to culture various bacteria and fungi found in the soil. This agar can be acidified or loaded with antibiotics such as chloramphenicol to inhibit unwanted bacterial growth. PDA is used in the food industry to test for fungi that can spoil food products. It is also used in the pharmaceutical industry to screen compound libraries for potential antifungal agents.[3]
Potato dextrose agar is a versatile growing medium for bacteria and fungi (yeasts and molds). This agar is used for a broad range of fungi but there are other agars that are more selective for specific types of fungi. These agars include but are not limited to malt extract agar and sabouraud agar. Malt extract agar is more acidic than PDA and is commonly used to cultivate penicillium species.[4] Sabouraud agar is also slightly acidic with pH 5.6-6.0 which is similar to PDA. It is most often used for the isolation of pathogenic fungi such as dermatophytes.
Typical composition
value ingredients & conditions 1000 mL water (strained broth from 200 g of infused potato into the water above) potatoes
(sliced washed unpeeled)20 g dextrose 20 g agar powder 5.6±0.2 final pH 25°C temperature
Potato infusion can be made by boiling 200 grams (7.1 oz) of sliced (washed but unpeeled) potatoes in ~ 1 litre (0.22 imp gal; 0.26 US gal) distilled water for 30 minutes and then decanting or straining the broth through cheesecloth. Distilled water is added such that the total volume of the suspension is 1 litre (0.22 imp gal; 0.26 US gal). 20 grams (0.71 oz) dextrose and 20 grams (0.71 oz) agar powder is then added and the medium is sterilized by autoclaving at 15 pounds per square inch (100 kPa) for 15 minutes.[1]
A similar growth medium, potato dextrose broth (abbreviated "PDB"), is formulated identically to PDA, omitting the agar.[5] Common organisms that can be cultured on PDB are yeasts such as Candida albicans and Saccharomyces cerevisiae and molds such as Aspergillus niger.[5]
References
- ↑ 1.0 1.1 BAM Media M127: Potato Dextrose Agar from the U.S. Food and Drug Administration
- ↑ Harold Eddleman, Ph. D (February 1998). "Making Bacteria Media from Potato". Indiana Biolab. disknet.com. http://www.disknet.com/indiana_biolab/b029.htm.
- ↑ Pitt & Hocking (2009). Fungi and food spoilage. Springer Science & Business Media. Bibcode: 2009ffs..book.....P.
- ↑ "Atlas of clinical fungi". Centraalbureau voor Schimmelcultures (2). 2009.
- ↑ 5.0 5.1 "Potato Dextrose Broth". Merck KGaA. http://www.emdchemicals.com/analytics/Micro_Manual/TEDISdata/prods/1_00510_0500.html.
Further reading
- Atlas, R.M.: Handbook of Microbiological Media, second edition. Lawrence C. Parks (1997)
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