Biology:FASTK
 Generic protein structure example |
Fas-activated serine/threonine kinase is an enzyme that in humans is encoded by the FASTK gene.[1][2][3]
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates the apoptosis-promoting nuclear RNA-binding protein TIA1. The encoded protein is a strong inducer of lymphocyte apoptosis.
Two transcript variants encoding different isoforms have been found for this gene. The longer one is located in the cell nucleus and cytoplasm, and the shorter one is 34 amino acids at the N-terminus, which is transported to the mitochondrion.[4]
The mitochondrial FASTK isoform localizes in the mitochondrial granules, where it interacts with the GRSF1 protein and binds to mt-mRNA ND6, the template of the only protein encoded on the light strand of the mitochondrial genome. In the case of FASTK deletion, it was observed loss of ND6 mt-mRNA and a 50-60% decrease in the activity of complex I of the respiratory chain. However, overexpression of this protein resulted in the stabilization of ND6 mt-mRNA [4][5]
Other variants exist, but their full-length natures have not yet been determined.[3]
References
- ↑ "Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis". The Journal of Experimental Medicine 182 (3): 865–874. September 1995. doi:10.1084/jem.182.3.865. PMID 7544399.
- ↑ "FAST is a survival protein that senses mitochondrial stress and modulates TIA-1-regulated changes in protein expression". Molecular and Cellular Biology 24 (24): 10718–10732. December 2004. doi:10.1128/MCB.24.24.10718-10732.2004. PMID 15572676.
- ↑ 3.0 3.1 "Entrez Gene: FASTK Fas-activated serine/threonine kinase". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10922.
- ↑ 4.0 4.1 "The FASTK family of proteins: emerging regulators of mitochondrial RNA biology". Nucleic Acids Research 45 (19): 10941–10947. November 2017. doi:10.1093/nar/gkx772. PMID 29036396.
- ↑ "A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function". Cell Reports 10 (7): 1110–1121. February 2015. doi:10.1016/j.celrep.2015.01.063. PMID 25704814.
Further reading
- "[IMAGE: molecular integration of the analysis of the human genome and its expression]". Comptes Rendus de l'Académie des Sciences. Série III, Sciences de la Vie 318 (2): 263–272. February 1995. PMID 7757816.
- "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–174. January 1994. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research 6 (9): 791–806. September 1996. doi:10.1101/gr.6.9.791. PMID 8889548.
- "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–156. October 1997. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- "FAST is a BCL-X(L)-associated mitochondrial protein". Biochemical and Biophysical Research Communications 318 (1): 95–102. May 2004. doi:10.1016/j.bbrc.2004.03.188. PMID 15110758.
- "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–1178. October 2005. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
- "Transcriptome analysis of human gastric cancer". Mammalian Genome 16 (12): 942–954. December 2005. doi:10.1007/s00335-005-0075-2. PMID 16341674.
- "Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing". The Journal of Biological Chemistry 282 (3): 1539–1543. January 2007. doi:10.1074/jbc.C600198200. PMID 17135269.
- "Fas-activated serine/threonine phosphoprotein (FAST) is a regulator of alternative splicing". Proceedings of the National Academy of Sciences of the United States of America 104 (27): 11370–11375. July 2007. doi:10.1073/pnas.0704964104. PMID 17592127. Bibcode: 2007PNAS..10411370S.
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