Biology:NEK2

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

Serine/threonine-protein kinase Nek2 is an enzyme that in humans is encoded by the NEK2 gene.

Interactions

NEK2 has been shown to interact with MAPK1 and NDC80. Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not [Uniprot].

Further reading

• "Nek2 kinase in chromosome instability and cancer.". Cancer Lett. 237 (2): 155–66. 2006. doi:10.1016/j.canlet.2005.06.017. PMID 16084011. 
• "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans.". Cell Growth Differ. 5 (6): 625–35. 1994. PMID 7522034. 
• "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. 1994. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. 1997. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• "A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators". EMBO J. 17 (2): 470–81. 1998. doi:10.1093/emboj/17.2.470. PMID 9430639. 
• "C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2". J. Cell Biol. 141 (7): 1563–74. 1998. doi:10.1083/jcb.141.7.1563. PMID 9647649. 
• "Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif". J. Biol. Chem. 274 (23): 16304–10. 1999. doi:10.1074/jbc.274.23.16304. PMID 10347187. 
• "A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41". Genome Res. 10 (1): 81–94. 2000. doi:10.1101/gr.10.1.81. PMID 10645953. 
• "NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1". Biochem. J. 349 (Pt 2): 509–18. 2001. doi:10.1042/0264-6021:3490509. PMID 10880350. 
• "Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis". Biochem. J. 361 (Pt 1): 77–85. 2002. doi:10.1042/0264-6021:3610077. PMID 11742531. 
• "Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle". Biochem. Biophys. Res. Commun. 290 (2): 730–6. 2002. doi:10.1006/bbrc.2001.6212. PMID 11785960. 
• "Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation". J. Biol. Chem. 277 (46): 44013–20. 2003. doi:10.1074/jbc.M208035200. PMID 12221103. 
• "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. 277 (51): 49408–16. 2003. doi:10.1074/jbc.M207069200. PMID 12386167. 
• "Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles". Mol. Biol. Cell 14 (7): 2876–89. 2004. doi:10.1091/mbc.E03-02-0108. PMID 12857871. 
• "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". J. Biol. Chem. 279 (19): 20049–57. 2004. doi:10.1074/jbc.M314205200. PMID 14978040. 
• "Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells". J. Biol. Chem. 279 (31): 32716–27. 2004. doi:10.1074/jbc.M404104200. PMID 15161910. 
• "Lineage-specific gene duplication and loss in human and great ape evolution". PLOS Biol. 2 (7): E207. 2006. doi:10.1371/journal.pbio.0020207. PMID 15252450. 
• "Nek2A specifies the centrosomal localization of Erk2". Biochem. Biophys. Res. Commun. 321 (2): 495–501. 2004. doi:10.1016/j.bbrc.2004.06.171. PMID 15358203.