Biology:Methanosarcina sRNAs
sRNA162, sRNA154, sRNA41 are small non-coding RNA (sRNA) identified together with 248 other sRNA candidates by RNA sequencing in methanogenic archaeon Methanosarcina mazei Gö1.[1] These sRNAs were further characterised. It was shown that sRNA162 can interact with both, a cis- and a trans-encoded mRNAs using two distinct domains. The sRNA overlaps the 5′UTR of the MM2442 mRNA and acts as a cis-encoded antisense RNA, and it also regulates MM2441 expression as a trans-encoded sRNA. It exhibits a regulatory role in the metabolic switch between methanol and trimethylamine as carbon and energy source. [2] sRNA154, exclusively expressed under nitrogen deficiency, has a central regulatory role in nitrogen metabolism affecting nitrogenase and glutamine synthetase by masking the ribosome binding site or positively affecting transcript stability. [3] sRNA41, highly expressed during nitrogen sufficiency, is capable to bind several ribosome binding sites independently within a polycistronic mRNA. It was proposed to inhibits translation initiation of all ACDS (acetyl-CoA decarbonylase/synthase complex) genes in N-dependent manner. [4]
See also
Bacterial sRNA involved in nitrogen metabolism: NsiR4
Other archaeal sRNAs:
- Pyrobaculum asR3 small RNA
- Archaeal H/ACA sRNA
References
- ↑ "Deep sequencing analysis of the Methanosarcina mazei Gö1 transcriptome in response to nitrogen availability". Proceedings of the National Academy of Sciences of the United States of America 106 (51): 21878–21882. December 2009. doi:10.1073/pnas.0909051106. PMID 19996181. Bibcode: 2009PNAS..10621878J.
- ↑ "An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains". Nucleic Acids Research 40 (21): 10964–10979. November 2012. doi:10.1093/nar/gks847. PMID 22965121.
- ↑ "154 a newly identified regulator of nitrogen fixation in Methanosarcina mazei strain Gö1". RNA Biology 14 (11): 1544–1558. November 2017. doi:10.1080/15476286.2017.1306170. PMID 28296572.
- ↑ "41 affects ribosome binding sites within polycistronic mRNAs in Methanosarcina mazei Gö1". Molecular Microbiology 107 (5): 595–609. March 2018. doi:10.1111/mmi.13900. PMID 29271512.
Original source: https://en.wikipedia.org/wiki/Methanosarcina sRNAs.
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