Biology:2-oxopropyl-CoM reductase (carboxylating)
| 2-oxopropyl-CoM reductase (carboxylating) | |||||||||
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| Identifiers | |||||||||
| EC number | 1.8.1.5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a 2-oxopropyl-CoM reductase (carboxylating) (EC 1.8.1.5) is an enzyme that catalyzes the chemical reaction
- 2-mercaptoethanesulfonate + acetoacetate + NADP+ [math]\displaystyle{ \rightleftharpoons }[/math] 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH
The 3 substrates of this enzyme are 2-mercaptoethanesulfonate, acetoacetate, and NADP+, whereas its 3 products are 2-(2-oxopropylthio)ethanesulfonate, CO2, and NADPH.
This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-mercaptoethanesulfonate, acetoacetate:NADP+ oxidoreductase (decarboxylating). Other names in common use include NADPH:2-(2-ketopropylthio)ethanesulfonate, oxidoreductase/carboxylase, and NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2C3C and 2C3D.
References
- "A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation". Proc. Natl. Acad. Sci. U.S.A. 96 (15): 8432–7. 1999. doi:10.1073/pnas.96.15.8432. PMID 10411892. Bibcode: 1999PNAS...96.8432A.
- "Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system". Biochemistry 39 (6): 1294–304. 2000. doi:10.1021/bi992282p. PMID 10684609.
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