Biology:Aeropyrum pernix
| Aeropyrum pernix | |
|---|---|
Scientific classification 
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| Domain: | Archaea |
| Kingdom: | Proteoarchaeota |
| Superphylum: | TACK group |
| Phylum: | Thermoproteota |
| Class: | Thermoprotei |
| Order: | Desulfurococcales |
| Family: | Desulfurococcaceae |
| Genus: | Aeropyrum |
| Species: | A. pernix
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| Binomial name | |
| Aeropyrum pernix Sako et al. 1996
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Aeropyrum pernix is a species of extremophile archaea in the archaeal phylum Thermoproteota. It is an obligatorily thermophilic species. The first specimens were isolated from sediments in the sea off the coast of Japan .
Discovery
Aeropyrum pernix was the first strictly aerobic hyperthermophilic Archaea to be discovered. It was originally isolated from heated marine sediments and venting water collected in 1996 from a solfataric vent at Kodakara-jima Island in Kyūshū, Japan .[1]
Genome structure
Its complete genome was sequenced in 1999 and is 1,669 kilobases in size, with 2,694 possible genes detected.[2] All of the genes in the TCA cycle were found except for that of α-ketoglutarate dehydrogenase. In its place, the genes coding for the two subunits of 2-oxoacid:ferredoxin oxidoreductase were identified.
Properties
The cells of Aeropyrum pernix are spherical in shape and approximately 1 μm in diameter. The envelope surrounding the cells of Aeropyrum is about 25 nm wide. The organisms grows at temperature between 70 and 100 °C (optimum, 90 to 95 °C), at pH 5 to 9 (optimum, pH 7), and at a salinity of 1.8 to 7% (optimum, 3.5% salinity). The growth of the organisms is not detected at 68 or 102 °C. Below 1.5% salinity, cells lyse by low osmotic shock. The cells of the organisms are sensitive to chloramphenicol and insensitive to ampicillin, vancomycin, and cycloserine. It grows well on proteinaceous substances, with a doubling time under these conditions of about 200 minutes.[1] This species lacks the genes for purine nucleotide biosynthesis and thus relies on environmental sources to meet its purine requirements.[3]
References
- ↑ 1.0 1.1 Sako Y; Nomura N; Uchida A et al. (1996). "Aeropyrum pernix gen. nov., sp. nov., a novel aerobic hyperthermophilic archaeon growing at temperatures up to 100 degrees C". Int. J. Syst. Bacteriol. 46 (4): 1070–7. doi:10.1099/00207713-46-4-1070. PMID 8863437.
- ↑ Kawarabayasi Y; Hino Y; Horikawa H et al. (1999). "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1". DNA Res. 6 (2): 83–101, 145–52. doi:10.1093/dnares/6.2.83. PMID 10382966.
- ↑ Brown, Anne M; Hoopes, Samantha L; White, Robert H; Sarisky, Catherine A (2011-12-14). "Purine biosynthesis in archaea: variations on a theme". Biology Direct 6: 63. doi:10.1186/1745-6150-6-63. ISSN 1745-6150. PMID 22168471.
Further reading
- Bhuiya, Mohammad Wadud; Suryadi, Jimmy; Zhou, Zholi; Brown, Bernard Andrew II (5 August 2013). "Structure of the Aeropyrum pernix L7Ae multifunctional protein and insight into its extreme thermostability". Acta Crystallographica Section F 69 (Pt 9): 979–988. doi:10.1107/S1744309113021799. PMID 23989144.
- Daifuku, Takashi; Yoshida, Takashi; Takayuki, Kitamura; Kawaichi, Satoshi; Inoue, Takahiro; Nomura, Keigo; Yoshida, Yui; Kuno, Sotaro et al. (19 July 2013). "Variation of the Virus-Related Elements within Syntenic Genomes of the Hyperthermophilic Archaeon Aeropyrum". Appl. Environ. Microbiol. 79 (19): 5891–8. doi:10.1128/AEM.01089-13. PMID 23872576. Bibcode: 2013ApEnM..79.5891D.
- Lee, Pyung Cheon; Mijts, Benjamin N.; Petri, Ralf; Watts, Kevin T.; Schmidt-Dannert, Claudia (16 June 2004). "Alteration of product specificity of Aeropyrum pernix farnesylgeranyl diphosphate synthase (Fgs) by directed evolution". Protein Engineering Design & Selection 17 (11): 771–777. doi:10.1093/protein/gzh089. PMID 15548566.
- Ota, Ajda; Gmajner, Dejan; Sentijurc, Marjeta; Ulrih, Natasa Poklar (13 April 2012). "Effect of Growth Medium pH of Aeropyrum peering on Structural Properties and Fluidity of Archaeosomes". Archaea 2012: 9. doi:10.1155/2012/285152. PMID 22778670.
- Sakuraba, Haruhiko; Satoura, Takenori; Kawakam, Ryushi; Kim, Kwang; Kara, Yusuke; Yoneda, Kazunari; Ohshima, Toshihisa (April 16, 2012). "Crystal Structure of Novel Dye- linked L-Proline Dehydrogenase from Hyperthermophilic Archaeon Aeropyrum Pernix". The Journal of Biological Chemistry 287 (24): 20070–20080. doi:10.1074/jbc.M111.319038. PMID 22511758.
External links
- Type strain of Aeropyrum pernix at BacDive - the Bacterial Diversity Metadatabase
- KEGG Genome : Aeropyrum pernix
Wikidata ☰ Q4688419 entry
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