Biology:Colicin

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Short description: Type of bacteriocin produced by and toxic to some strains of Escherichia coli
Colicin
PDB 2ivz EBI.jpg
Structure of TolB in complex with a peptide of the colicin e9 t-domain
Identifiers
SymbolColicin
PfamPF03515
Pfam clanCL0446
InterProIPR003058
SCOP21jch / SCOPe / SUPFAM

A colicin is a type of bacteriocin produced by and toxic to some strains of Escherichia coli.[1] Colicins are released into the environment to reduce competition from other bacterial strains. Colicins bind to outer membrane receptors, using them to translocate to the cytoplasm or cytoplasmic membrane, where they exert their cytotoxic effect, including depolarisation of the cytoplasmic membrane, DNase activity, RNase activity, or inhibition of murein synthesis.

Structure

Channel-forming colicins (colicins A, B, E1, Ia, Ib, and N) are transmembrane proteins that depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.[2] These colicins contain at least three domains: an N-terminal translocation domain responsible for movement across the outer membrane and periplasmic space (T domain); a central domain responsible for receptor recognition (R domain); and a C-terminal cytotoxic domain responsible for channel formation in the cytoplasmic membrane (C domain).[3][4][5] R domain regulates the target and binds to the receptor on the sensitive cell. T domain is involved in translocation, co-opting the machinery of the target cell. The C domain is the 'killing' domain and may produce a pore in the target cell membrane, or act as a nuclease to chop up the DNA or RNA of the target cell.[5]

Translocation

Most colicins are able to translocate the outer membrane by a two-receptor system, where one receptor is used for the initial binding and the second for translocation. The initial binding is to cell surface receptors such as the outer membrane proteins OmpF, FepA, BtuB, Cir and FhuA; colicins have been classified according to which receptors they bind to. The presence of specific periplasmic proteins, such as TolA, TolB, TolC, or TonB, are required for translocation across the membrane.[6] Cloacin[7] DF13 is a bacteriocin that inactivates ribosomes by hydrolysing 16S RNA in 30S ribosomes at a specific site.[8]

Resistance

Because they target specific receptors and use specific translocation machinery, cells can make themselves resistant to the colicin by repressing or deleting the genes for these proteins. Such resistant cells may suffer the lack of a key nutrient (such as iron or a B vitamin), but benefit by not being killed. Colicins exhibit a '1-hit killing kinetic' [citation needed] which does not necessarily mean a single molecule is sufficient to kill, but certainly that it only takes a small number. In his 1969 Nobel Laureate speech, Salvador E. Luria speculated that colicins could only be this toxic by causing a domino effect that destabilized the cell membrane.[9] He was not entirely correct, but pore-forming colicins do depolarize the membrane and thus eliminate the energy source for the cell. The colicins are highly effective toxins.[citation needed]

Genetic organisation

Virtually all colicins are carried on plasmids. The two general classes of colicinogenic plasmids are large, low-copy-number plasmids, and small, high-copy-number plasmids. The larger plasmids carry other genes, as well as the colicin operon. The colicin operons are generally organized with several major genes. These include a colicin structural gene, an immunity gene, and a bacteriocin release protein (BRP), or lysis, gene. The immunity gene is often produced constitutively, while the BRP is generally produced only as a read-through of the stop codon on the colicin structural gene. The colicin itself is repressed by the SOS response and may be regulated in other ways as well.[10]

Retaining the colicin plasmid is very important for cells that live with their relatives, because if a cell loses the immunity gene, it quickly becomes subject to destruction by circulating colicin. At the same time, colicin is only released from a producing cell by the use of the lysis protein, which results in that cell's death. This suicidal production mechanism would appear to be very costly, except for the fact that it is regulated by the SOS response, which responds to significant DNA damage. In short, colicin production may only occur in terminally ill cells. The Professor Kleanthous Research Group at the University of Oxford study colicins extensively as a model system for characterising and investigating protein-protein interactions and recognition.[11]

BACTIBASE[12][13] database is an open-access database for bacteriocins including colicins (view complete list).

References

  1. ↑ "The phenotypic and fitness effects of colicin resistance in Escherichia coli K-12". Evolution 53 (4): 1019–27. 1999. doi:10.2307/2640807. PMID 28565527. 
  2. ↑ "Crystal structure of the cytotoxic bacterial protein colicin B at 2.5 A resolution". Mol. Microbiol. 51 (3): 711–20. 2004. doi:10.1111/j.1365-2958.2003.03884.x. PMID 14731273. 
  3. ↑ "On the role of lipid in colicin pore formation". Biochim. Biophys. Acta 1666 (1): 239–49. 2004. doi:10.1016/j.bbamem.2004.07.001. PMID 15519318. 
  4. ↑ Cascales et al. (2007). Colicin Biology. Microbio. and Mol. Bio. Rev. 71(1), 158-229. Abstract pdf
  5. ↑ 5.0 5.1 Wiener, Michael; Freymann, Douglas; Ghosh, Partho; Stroud, Robert M. (January 1997). "Crystal structure of colicin Ia" (in en). Nature 385 (6615): 461–464. doi:10.1038/385461a0. ISSN 1476-4687. PMID 9009197. Bibcode: 1997Natur.385..461W. https://www.nature.com/articles/385461a0. 
  6. ↑ "Mechanisms of colicin binding and transport through outer membrane porins". Biochimie 84 (5–6): 399–412. 2002. doi:10.1016/S0300-9084(02)01455-4. PMID 12423783. 
  7. ↑ "Molecular structure of the immunity gene and immunity protein of the bacteriocinogenic plasmid Clo DF13". Nucleic Acids Res. 8 (19): 4349–63. October 1980. doi:10.1093/nar/8.19.4349. PMID 6253914. 
  8. ↑ "Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid Clo DF13". Nucleic Acids Res. 11 (8): 2465–2477. 1983. doi:10.1093/nar/11.8.2465. PMID 6344017. 
  9. ↑ Luria, S. E. (1969) Nobel Lecture
  10. ↑ Mader, Andreas; von Bronk, Benedikt; Ewald, Benedikt; Kesel, Sara; Schnetz, Karin; Frey, Erwin; Opitz, Madeleine (2015-03-09). "Amount of Colicin Release in Escherichia coli Is Regulated by Lysis Gene Expression of the Colicin E2 Operon". PLOS ONE 10 (3): e0119124. doi:10.1371/journal.pone.0119124. ISSN 1932-6203. PMID 25751274. Bibcode: 2015PLoSO..1019124M. 
  11. ↑ "Kleanthous Research Group". http://www.bioch.ox.ac.uk/aspsite/index.asp?pageid=910. Retrieved 23 May 2017. 
  12. ↑ "BACTIBASE: a new web-accessible database for bacteriocin characterization". BMC Microbiology 7: 89. 2007. doi:10.1186/1471-2180-7-89. PMID 17941971. 
  13. ↑ "BACTIBASE second release: a database and tool platform for bacteriocin characterization.". BMC Microbiology 10: 22. 2010. doi:10.1186/1471-2180-10-22. PMID 20105292. 

External links

This article incorporates text from the public domain Pfam and InterPro: IPR000290



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