Biology:Missense mRNA
Missense mRNA is a messenger RNA bearing one or more mutated codons that yield polypeptides with an amino acid sequence different from the wild-type or naturally occurring polypeptide.[1] Missense mRNA molecules are created when template DNA strands or the mRNA strands themselves undergo a missense mutation in which a protein coding sequence is mutated and an altered amino acid sequence is coded for.
Biogenesis
A missense mRNA arises from a missense mutation, in the event of which a DNA nucleotide base pair in the coding region of a gene is changed such that it results in the substitution of one amino acid for another.[2] The point mutation is nonsynonymous because it alters the RNA codon in the mRNA transcript such that translation results in amino acid change. An amino acid change may not result in appreciable changes in protein structure depending on whether the amino acid change is conservative or non-conservative. This owes to the similar physicochemical properties exhibited by some amino acids.[3]
Missense mRNAs may be detected as a result of two different types of point mutations - spontaneous mutations and induced mutations.[4] Spontaneous mutations occur during the DNA replication process where a non-complementary nucleotide is deposited by the DNA polymerase in the extension phase. The consecutive round of replication would result in a point mutation. If the resulting mRNA codon is one that changes the amino acid, a missense mRNA would be detected. A hypergeometric distribution study involving DNA polymerase β replication errors in the APC gene revealed 282 possible substitutions that could result in missense mutations. When the APC mRNA was analyzed in the mutational spectrum, it showed 3 sites where the frequency of substitutions were high. [5]
Induced mutations caused by mutagens can give rise to missense mutations.[4] Nucleoside analogues such as 2-aminopurine and 5-bromouracil can insert in place of A and T respectively. Ionizing radiation like x-rays and γ-rays can deaminate cytosine to uracil.[6]
Missense mRNAs may be applied synthetically in forward and reverse genetic screens used to interrogate the genome. Site-directed mutagenesis is a technique often employed to create knock-in and knock-out models that express missense mRNAs. For example, in knock-in studies, human orthologs are identified in model organisms to introduce missense mutations,[7] or a human gene with a substitution mutation is integrated into the genome of the model organism.[8] The subsequent loss-of-function or gain-of-function phenotypes are measured to model genetic diseases and discover novel drugs.[9] While homologous recombination has been widely used to generate single-base substitutions, novel technologies that co-inject gRNA and hCas9 mRNA of the CRISPR/Cas9 system, in conjunction with single-strand oligodeoxynucleotide (ssODN) donor sequences have shown efficiency in generating point mutations in the genome.[9][10][11]
Evolutionary implications
Non-synonymous RNA editing
Substitutions can occur on the level of both the DNA and RNA. RNA editing-dependent amino acid substitutions can produce missense mRNA's of which occur through hydrolytic deaminase reactions. Two of the most prevalent deaminase reactions occur through the Apolipoprotein B mRNA editing enzyme (APOBEC) and the adenosine deaminase acting on RNA enzyme (ADAR) which are responsible for the conversion of cytidine to uridine (C-to-U), and the deamination of adenosine to inosine (A-to-I), respectively. [12] Such selective substitutions of uridine for cytidine, and inosine for adenosine in RNA editing can produce differential isoforms of missense mRNA transcripts, and confer transcriptome diversity and enhanced protein function in response to selective pressures. [13]
See also
References
- ↑ Jameson, J. Larry. Principles of Molecular Medicine. Springer. pp. 731.
- ↑ "Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping". Molecular and Cellular Biology 14 (9): 6326–36. September 1994. doi:10.1128/mcb.14.9.6326. PMID 8065364.
- ↑ "Missense Mutation" (in en). https://www.genome.gov/genetics-glossary/Missense-Mutation.
- ↑ 4.0 4.1 Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James (2000). "Mutations: Types and Causes" (in en). Molecular Cell Biology. 4th Edition. https://www.ncbi.nlm.nih.gov/books/NBK21578/.
- ↑ "The DNA polymerase beta replication error spectrum in the adenomatous polyposis coli gene contains human colon tumor mutational hotspots". Cancer Research 62 (11): 3271–5. June 2002. PMID 12036944. https://cancerres.aacrjournals.org/content/62/11/3271.
- ↑ "Mutations | Microbiology". https://courses.lumenlearning.com/microbiology/chapter/mutations/.
- ↑ "Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders". Disease Models & Mechanisms 11 (10): dmm035469. October 2018. doi:10.1242/dmm.035469. PMID 30355756.
- ↑ "A targeted Coch missense mutation: a knock-in mouse model for DFNA9 late-onset hearing loss and vestibular dysfunction". Human Molecular Genetics 17 (21): 3426–34. November 2008. doi:10.1093/hmg/ddn236. PMID 18697796.
- ↑ 9.0 9.1 "Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs". Scientific Reports 9 (1): 4811. March 2019. doi:10.1038/s41598-019-41121-4. PMID 30886178. Bibcode: 2019NatSR...9.4811O.
- ↑ "Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system". Scientific Reports 4: 5396. June 2014. doi:10.1038/srep05396. PMID 24953798. Bibcode: 2014NatSR...4E5396I.
- ↑ "Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9". Nucleic Acids Research 46 (17): e102. September 2018. doi:10.1093/nar/gky512. PMID 29905858.
- ↑ "RNA Editing-Systemic Relevance and Clue to Disease Mechanisms?" (in English). Frontiers in Molecular Neuroscience 9: 124. 2016. doi:10.3389/fnmol.2016.00124. PMID 27932948.
- ↑ "The evolution and adaptation of A-to-I RNA editing". PLOS Genetics 13 (11): e1007064. November 2017. doi:10.1371/journal.pgen.1007064. PMID 29182635.
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