Biology:Multisite-specific tRNA:(cytosine-C5)-methyltransferase
From HandWiki
| Multisite-specific tRNA:(cytosine-C5)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.202 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Multisite-specific tRNA:(cytosine-C5)-methyltransferase (EC 2.1.1.202, multisite-specific tRNA:m5C-methyltransferase, TRM4 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase.[1][2][3][4] This enzyme catalyses the following chemical reaction
- (1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
- (2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor
- (3) S-adenosyl-L-methionine + cytosine48 in tRNA [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
- (4) S-adenosyl-L-methionine + cytosine49 in tRNA [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
References
- ↑ "Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme". RNA 5 (8): 1105–18. August 1999. doi:10.1017/s1355838299982201. PMID 10445884.
- ↑ "Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study". Nucleic Acids Research 25 (14): 2694–701. July 1997. doi:10.1093/nar/25.14.2694. PMID 9207014.
- ↑ "Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo". Molecular and Cellular Biology 6 (7): 2663–73. July 1986. doi:10.1128/mcb.6.7.2663. PMID 3537724.
- ↑ "Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase". RNA 13 (7): 967–73. July 2007. doi:10.1261/rna.515707. PMID 17475914.
External links
- Multisite-specific+tRNA:(cytosine-C5)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)
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