Biology:RRNA small subunit pseudouridine methyltransferase Nep1
From HandWiki
| RRNA small subunit pseudouridine methyltransferase Nep1 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.260 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
RRNA small subunit pseudouridine methyltransferase Nep1 (EC 2.1.1.260, Nep1, nucleolar essential protein 1) is an enzyme with systematic name S-adenosyl-L-methionine:18S rRNA (pseudouridine1191-N1)-methyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
- S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
This enzyme recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure.
A point mutation in the ribosome biogenesis factor Nep1 impairs its nucleolar localisation and RNA binding and causes the Bowen-Conradi syndrome. [4] .[5]
References
- ↑ "The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site". Nucleic Acids Research 36 (5): 1542–54. March 2008. doi:10.1093/nar/gkm1172. PMID 18208838.
- ↑ "The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase". Nucleic Acids Research 38 (7): 2387–98. April 2010. doi:10.1093/nar/gkp1189. PMID 20047967.
- ↑ "The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA". Nucleic Acids Research 39 (4): 1526–37. March 2011. doi:10.1093/nar/gkq931. PMID 20972225.
- ↑ Meyer B, Wurm JP, Kotter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wöhnert J, Entian KD (2011). "The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Psi 1191 in yeast 18S rRNA". Nucleic Acids Res 39 (4): 1526–37. doi:10.1093/nar/gkq931. PMID 20972225.
- ↑ Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kotter P, Engels JW, Heckel A, Karas M, Entian KD, Wöhnert J (2010). "The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase". Nucleic Acids Res 38 (7): 2387–98. doi:10.1093/nar/gkp1189. PMID 20047967.
External links
- RRNA+small+subunit+pseudouridine+methyltransferase+Nep1 at the US National Library of Medicine Medical Subject Headings (MeSH)
 |  |
