Biology:Site-specific DNA-methyltransferase (adenine-specific)
From HandWiki
| Site-specific DNA-methyltransferase (adenine-specific) | |||||||||
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| Identifiers | |||||||||
| EC number | 2.1.1.72 | ||||||||
| CAS number | 69553-52-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Site-specific DNA-methyltransferase (adenine-specific) (EC 2.1.1.72, modification methylase, restriction-modification system) is an enzyme.[1][2][3] This enzyme catalyses the following chemical reaction
- S-adenosyl-L-methionine + DNA adenine [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
This is a large group of enzymes.[4]
The E. coli DNA adenine methyltransferase enzyme (Dam), is widely used for the chromatin profiling technique, DamID. In which the Dam is fused to a DNA-binding protein of interest and expressed as a transgene in a genetically tractable model organism to identify protein binding sites.[5]
See also
References
- ↑ "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene 92 (1–2): 1–248. August 1990. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
- ↑ "Restriction enzymes and their isoschizomers". Nucleic Acids Research 18 Suppl: 2331–65. April 1990. doi:10.1093/nar/18.suppl.2331. PMID 2159140.
- ↑ "Structure and mechanism of multifunctional restriction endonucleases". Annual Review of Biochemistry 50: 285–319. 1981. doi:10.1146/annurev.bi.50.070181.001441. PMID 6267988.
- ↑ "The Restriction Enzyme Database". http://rebase.neb.com/rebase/rebase.html.
- ↑ "Dam it's good! DamID profiling of protein-DNA interactions". Wiley Interdisciplinary Reviews: Developmental Biology 5 (1): 25–37. January 2016. doi:10.1002/wdev.205. PMID 26383089.
External links
- Site-specific+DNA-methyltransferase+(adenine-specific) at the US National Library of Medicine Medical Subject Headings (MeSH)
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