Biology:Sakaguchi test: Difference between revisions
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Short description: Chemical test used to detect presence of arginine in proteins
The Sakaguchi test is a chemical test used to detect presence of arginine in proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.[1] The Sakaguchi reagent used in the test consists of 1-Naphthol and a drop of sodium hypobromite. The guanidino (–C group in arginine reacts with the Sakaguchi reagent to form a red-coloured complex.[2][3][4][5]
References
- ↑ Sakaguchi, Shoyo (April 1925). "Über eine neue Farbenreaktion von protein und arginin" (in de). Journal of Biochemistry (Oxford University Press; Japanese Biochemical Society) 5: 25–31. doi:10.1093/oxfordjournals.jbchem.a128359. https://academic.oup.com/jb/article-abstract/5/1/25/2183654.
- ↑ Chatterjea (1 January 2004). Textbook of Biochemistry for Dental/Nursing/Pharmacy Students. Jaypee Brothers Publishers. p. 51. ISBN 978-81-8061-204-6. https://books.google.com/books?id=xN0YYypnZVkC&pg=PA51.
- ↑ R.A. Joshi (2006). Question Bank of Biochemistry. New Age International. p. 64. ISBN 978-81-224-1736-4. https://books.google.com/books?id=Acf9BkEqJWYC&pg=PA64.
- ↑ Dandekar (1 January 2004). Practicals And Viva In Medical Biochemistry. Elsevier India. p. 28. ISBN 978-81-8147-025-6. https://books.google.com/books?id=s9HEoJquruwC&pg=PA28.
- ↑ Joshi, Rashmi A.; Marks, Victor (30 June 2004) (in en). Guide to Bio-chemistry. B. Jain Publishers. p. 74. ISBN 9788180560538. https://books.google.com/books?id=avDlDrPR4joC&dq=The+guanidine+group+in+arginine+reacts+with+the+Sakaguchi+reagent+to+form+a+red-coloured+complex&pg=PA74.
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