Biology:Phosphoribosylglycinamide formyltransferase

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Phosphoribosylglycinamide formyltransferase
1rby.jpg
GAR formyltransferase monomer, Human
Identifiers
EC number2.1.2.2
CAS number2604945
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2, 2-amino-N-ribosylacetamide 5'-phosphate transformylase, GAR formyltransferase, GAR transformylase, glycinamide ribonucleotide transformylase, GAR TFase, 5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase) is an enzyme with systematic name 10-formyltetrahydrofolate:5'-phosphoribosylglycinamide N-formyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide [math]\displaystyle{ \rightleftharpoons }[/math] tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
Overall reaction of GAR transformylase

This THF dependent enzyme catalyzes a nucleophilic acyl substitution of the formyl group from 10-formyltetrahydrofolate (fTHF) to N1-(5-phospho-D-ribosyl)glycinamide (GAR) to form N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide (fGAR) as shown above.[4] This reaction plays an important role in the formation of purine through the de novo purine biosynthesis pathway. This pathway creates inosine monophosphate (IMP), a precursor to adenosine monophosphate (AMP) and guanosine monophosphate (GMP). AMP is a building block for important energy carriers such as ATP, NAD+ and FAD, and signaling molecules such as cAMP. GARTfase's role in de novo purine biosynthesis makes it a target for anti-cancer drugs[5] and its overexpression during postnatal development has been connected to Down syndrome.[6] There are two known types of genes encoding GAR transformylase in E.coli: purN and purT, while only purN is found in humans.[7] Many residues in the active site are conserved across bacterial, yeast, avian and human enzymes.[8]

Enzyme Structure

Structure of GAR transformylase in rainbow from N(blue)->C(red). Folate binding loop is highlighted in black. GAR substrate (red) and THF analogue (blue) are shown in binding pockets.[9]

In humans, GARTfase is part of trifunctional enzyme which also includes glycinamide ribonucleotide synthase (GARS) and aminoimidazole ribonucleotide synthetase (AIRS). This protein (110kDa) catalyzes steps 2, 3 and 5 of de novo purine biosynthesis. The proximity of these enzyme units and flexibility of the protein serves to increase pathway throughput. GARTfase is located on the C-terminal end of the protein.[10]

Human GARTfase has been crystallized by vapor-diffusion sitting drop method and imaged at the Stanford Synchrotron Radiation Laboratory (SSRL) by at least two groups.[5][11]

The structure can be described by two subdomains which are connected by a seven-stranded beta sheet. The N- terminal domain consists of a Rossman type mononucleotide fold, with a four strand part of the beta sheet surrounded on each side by two alpha helices. The beta sheet continues into the C terminal domain, where on one side it is covered by a long alpha helix and on the other it is partially exposed to solvent. It is the cleft between the two subdomains where the active site lies.[8]

The cleft consists of the GAR binding site and the folate binding pocket. The folate binding pocket is delineated by pteridine-binding cleft, the formyl transfer region and the benzoylglutamate region which bind thepteridine head and a benzoylglutamate tail connected by a formyl bound nitrogen of fTHF. This folate binding region has been the subject of much research because its inhibition by small molecules has led to the discovery of antineoplastic drugs. The folate binding loop has been shown to change conformation depending on the pH of solution and as such Human GAR transformylase shows highest activity around pH 7.5-8. Lower pH (~4.2) conditions change the conformation of the substrate (GAR) binding loops as well.[11]

Mechanism

Mechanism of purN GARTfase

GAR transformylase water assisted mechanism suggested by Qiao et al

Klein et al first suggested a water molecule assisted mechanism. A single water molecule possibly held in place by hydrogen bonding with the carboxylate group of the persistent Asp144 residue transfers protons from the GAR-N to the THF-N. The nucleophilic nitrogen on the terminal amino group of GAR attacks the carbonyl carbon of the formyl group on THF pushing negative charge onto the oxygen. Klein suggests that His108 stabilizes the transition state by hydrogen bonding with the negatively charged oxygen and that the reformation of the carbonyl double bond results in breaking the THF-N - formyl bond. Calculations by Qiao et al suggest that the water assisted stepwise proton transfer from Gar-N to THF-N is 80-100 kj/mol more favorable than the concerted transfer suggested by Klein. The mechanism shown is suggested by Qiao et al, whom admittedly did not consider surrounding residues in their calculations.[12][13] Much of the early active site mapping on GAR TFase was determined with the bacterial enzyme owing to the quantity available from its overexpression in E. coli.[14] Using a bromoacetyl dideazafolate affinity analog James Inglese and colleagues first identified Asp144 as an active site residue likely involved in the formyl transfer mechanism.[15]

Mechanism of purT GARTfase

Studies of the purT variant of GAR transformylase in E.coli found that the reaction proceeds through a formyl phosphate intermediate. While the in vitro reaction can proceed without THF, overall the in vivo reaction is the same.[16]

Involvement in de novo Purine Biosynthesis

GART catalyzes the third step in de novo purine biosynthesis, the formation of N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide (fGAR) by formyl addition to N1-(5-phospho-D-ribosyl)glycinamide (GAR).[3] In E. coli, the purN enzyme is a 23 kDa protein[17] but in humans it is part of a trifunctional protein of 110 kDa which includes AIRS and GARS functionalities.[10] This protein catalyzes three different steps of the de novo purine pathway.

Disease Relevance

Cancer Target

Due to their increased growth rate and metabolic requirements, cancer cells rely on de novo nucleotide biosynthesis to achieve levels of AMP and GMP necessary.[18] Being able to block any of the steps of the de novo purine pathway would present significant reduction in tumor growth. Studies have been done both on the substrate binding[19] and folate binding site[20] to find inhibitors.

Down Syndrome

GARTfase is suspected to be connected with Down syndrome. The gene encoding the trifunctional protein human GARS-AIRS-GART is located on chromosome 21q22.1, in the Down syndrome critical region. The protein is overexpressed in the cerebellum during the postnatal development of individuals with Down syndrome. Typically, this protein is undetectable in cerebellum shortly after birth, but found in high levels in prenatal development.[6][21]

See also

  • Trifunctional purine biosynthetic protein adenosine-3

References

  1. ↑ "Biosynthesis of the purines. XXVI. The identification of the formyl donors of the transformylation reactions". The Journal of Biological Chemistry 234 (7): 1812–6. July 1959. PMID 13672969. 
  2. ↑ "L(-)-10-Formyltetrahydrofolate is the cofactor for glycinamide ribonucleotide transformylase from chicken liver". Biochemistry 20 (14): 4034–6. July 1981. doi:10.1021/bi00517a013. PMID 7284307. 
  3. ↑ 3.0 3.1 "Biosynthesis of the purines. XIX. 2-Amino-N-ribosylacetamide 5'-phosphate (glycinamide ribotide) transformylase". The Journal of Biological Chemistry 229 (2): 613–26. December 1957. PMID 13502326. 
  4. ↑ McMurry, J. and Tadhg, B. The Organic Chemistry of Biological Pathways
  5. ↑ 5.0 5.1 "Biological and structural evaluation of 10R- and 10S-methylthio-DDACTHF reveals a new role for sulfur in inhibition of glycinamide ribonucleotide transformylase". Biochemistry 52 (30): 5133–44. July 2013. doi:10.1021/bi4005182. PMID 23869564. 
  6. ↑ 6.0 6.1 "Potential interaction between the GARS-AIRS-GART Gene and CP2/LBP-1c/LSF transcription factor in Down syndrome-related Alzheimer disease". Cellular and Molecular Neurobiology 27 (8): 1117–26. December 2007. doi:10.1007/s10571-007-9217-2. PMID 17902044. 
  7. ↑ "Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli". Journal of Bacteriology 175 (11): 3591–7. June 1993. doi:10.1128/jb.175.11.3591-3597.1993. PMID 8501063. 
  8. ↑ 8.0 8.1 "Crystal structure of glycinamide ribonucleotide transformylase from Escherichia coli at 3.0 A resolution. A target enzyme for chemotherapy". Journal of Molecular Biology 227 (1): 283–92. September 1992. doi:10.1016/0022-2836(92)90698-j. PMID 1522592. 
  9. ↑ Zhang, Y., Desharnais, J., Boger, D.L., Wilson, I.A. (2005) "Human GAR Tfase complex structure with 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid and substrate beta-GAR." Unplubished. PDB: 1RBY.
  10. ↑ 10.0 10.1 "Structural studies of tri-functional human GART". Nucleic Acids Research 38 (20): 7308–19. November 2010. doi:10.1093/nar/gkq595. PMID 20631005. 
  11. ↑ 11.0 11.1 "Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR". Biochemistry 41 (48): 14206–15. December 2002. doi:10.1021/bi020522m. PMID 12450384. 
  12. ↑ "Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolution". Journal of Molecular Biology 249 (1): 153–75. May 1995. doi:10.1006/jmbi.1995.0286. PMID 7776369. 
  13. ↑ "A quantum chemical study on the mechanism of glycinamide ribonucleotide transformylase inhibitor: 10-Formyl-5,8,10-trideazafolic acid". Biophysical Chemistry 118 (2–3): 78–83. December 2005. doi:10.1016/j.bpc.2005.07.001. PMID 16198047. 
  14. ↑ "Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase". Biochemistry 29 (6): 1436–43. February 1990. doi:10.1021/bi00458a014. PMID 2185839. 
  15. ↑ "Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli". Biochemistry 29 (28): 6678–87. July 1990. doi:10.1021/bi00480a018. PMID 2204419. 
  16. ↑ "Formyl phosphate: a proposed intermediate in the reaction catalyzed by Escherichia coli PurT GAR transformylase". Biochemistry 36 (22): 6709–16. June 1997. doi:10.1021/bi962961p. PMID 9184151. 
  17. ↑ "Improvement in the efficiency of formyl transfer of a GAR transformylase hybrid enzyme". Protein Engineering 13 (5): 323–7. May 2000. doi:10.1093/protein/13.5.323. PMID 10835105. 
  18. ↑ "The molecular determinants of de novo nucleotide biosynthesis in cancer cells". Current Opinion in Genetics & Development 19 (1): 32–7. February 2009. doi:10.1016/j.gde.2009.01.002. PMID 19201187. 
  19. ↑ "Substrate specificity of human glycinamide ribonucleotide transformylase". Archives of Biochemistry and Biophysics 370 (2): 231–5. October 1999. doi:10.1006/abbi.1999.1428. PMID 10577357. 
  20. ↑ "Update on antifolate drugs targets". Current Drug Targets 2 (2): 135–66. June 2001. doi:10.2174/1389450013348669. PMID 11469716. 
  21. ↑ "The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome". Human Molecular Genetics 6 (12): 2043–50. November 1997. doi:10.1093/hmg/6.12.2043. PMID 9328467. 

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