Biology:tRNA (guanine-N2-)-methyltransferase
| tRNA (guanine-N2-)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.32 | ||||||||
| CAS number | 37217-31-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + tRNA [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + tRNA containing N2-methylguanine
Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N2-Methylguanine.
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N2-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 2-methyltransferase, transfer ribonucleate guanine N2-methyltransferase, transfer RNA guanine 2-methyltransferase, guanine-N2-methylase, and S-adenosyl-L-methionine:tRNA (guanine-2-N-)-methyltransferase.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2DUL, 2EJT, and 2EJU.
References
- "The specificity of transfer ribonucleic acid methylases from rat liver". Biochemistry 7 (1): 45–50. 1968. doi:10.1021/bi00841a007. PMID 4921283.
- "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9 (2): 207–15. 1969. doi:10.1111/j.1432-1033.1969.tb00596.x. PMID 4896260.
- "N2-Guanine specific transfer RNA methyltransferase I from rat liver and leukemic rat spleen*". Nucleic Acids Res. 1 (11): 1455–1478. 1974. doi:10.1093/nar/1.11.1455. PMID 10793703.
- "N2-Guanine specific transfer RNA methyltransferase II from rat liver". Nucleic Acids Res. 1 (11): 1479–1496. 1974. doi:10.1093/nar/1.11.1479. PMID 10793704.
- "tRNA methylases from HeLa cells: purification and properties of an adenine-1-methylase and a guanine-N2-methylase". Arch. Biochem. Biophys. 162 (1): 38–47. 1974. doi:10.1016/0003-9861(74)90102-7. PMID 4208689.
- "S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver". Nucleic Acids Res. 2 (10): 1639–51. 1975. doi:10.1093/nar/2.10.1639. PMID 1208211.
- "Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos". Biochemistry 16 (16): 3576–81. 1977. doi:10.1021/bi00635a012. PMID 19056.
- "Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver". Biochim. Biophys. Acta 518 (1): 158–71. 1978. doi:10.1016/0005-2787(78)90125-9. PMID 629973.
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