Biology:mRNA (nucleoside-2'-O-)-methyltransferase
| mRNA (nucleoside-2'-O-)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.57 | ||||||||
| CAS number | 61970-02-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + m7G(5')pppR-RNA [math]\displaystyle{ \rightleftharpoons }[/math] S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap)
Thus, the two substrates of this enzyme are S-adenosyl methionine and m7G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap).
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (nucleoside-2'-O-)-methyltransferase. Other names in common use include messenger ribonucleate nucleoside 2'-methyltransferase, and messenger RNA (nucleoside-2'-)-methyltransferase.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2GA9 and 2GAF.
References
- "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Purification and physical properties". J. Biol. Chem. 253 (21): 7692–7. 1978. doi:10.1016/S0021-9258(17)34425-3. PMID 701281.
- "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Characteristics and substrate specificity". J. Biol. Chem. 253 (21): 7698–702. 1978. doi:10.1016/S0021-9258(17)34426-5. PMID 701282.
- "Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus". J. Virol. 21 (2): 475–83. 1977. doi:10.1128/JVI.21.2.475-483.1977. PMID 833934.
- "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. 1975. doi:10.1073/pnas.72.7.2525. PMID 1058472. Bibcode: 1975PNAS...72.2525E.
- "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry 17 (6): 977–82. 1978. doi:10.1021/bi00599a005. PMID 629955.
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